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Int. J. Mol. Sci. 2014, 15(6), 11161-11171; doi:10.3390/ijms150611161
Article

Genetic Variations of TAP1 Gene Exon 3 Affects Gene Expression and Escherichia coli F18 Resistance in Piglets

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Received: 12 April 2014; in revised form: 22 May 2014 / Accepted: 27 May 2014 / Published: 20 June 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Firstly, our research group identified Sutai pigs’ phenotypes that exhibited extreme resistance and susceptibility to the Escherichia coli F18 respectively, and then eight ETEC (Enterotoxigenic Escherichia coli) F18-resistant piglets and eight ETEC F18-sensitive piglets were selected. Then, the TAP1 (Transporter associated with antigen processing) mRNA relative expression levels were analyzed in 11 tissues of the resistant and susceptible phenotypes. Simultaneously, we detected the genetic variations in exon 3 of the TAP1 gene and evaluated the TAP1 mRNA expression levels among the different genotype pigs to study the effects of the genetic variation on gene expression, and the E. coli F18 resistance. The results revealed higher expression levels in the resistant genotypes than that in the susceptible genotypes in 11 tissues, with significant differences in the spleen, lymph node, lung, thymus, duodenum and jejunum. Furthermore, a G729A mutation was identified in the TAP1 gene exon 3, and this mutation deviates from Hardy-Weinberg equilibrium (p < 0.01). The TAP1 mRNA levels in GG genotype were significantly higher than that in the other two genotypes, with significant differences in the liver, lung, kidney, thymus, lymph node, duodenum and jejunum tissues. We speculated that high expression of the TAP1 gene might confer resistance against the E. coli F18, the G729A mutation had a significant effect on the mRNA expression, and individuals with the GG genotype possessed a stronger ability to resist the E. coli F18 infection.
Keywords: piglets; TAP1 gene; E. coli F18; genetic variation; mRNA expression piglets; TAP1 gene; E. coli F18; genetic variation; mRNA expression
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zhao, Q.; Liu, Y.; Dong, W.; Zhu, S.; Huo, Y.; Wu, S.; Bao, W. Genetic Variations of TAP1 Gene Exon 3 Affects Gene Expression and Escherichia coli F18 Resistance in Piglets. Int. J. Mol. Sci. 2014, 15, 11161-11171.

AMA Style

Zhao Q, Liu Y, Dong W, Zhu S, Huo Y, Wu S, Bao W. Genetic Variations of TAP1 Gene Exon 3 Affects Gene Expression and Escherichia coli F18 Resistance in Piglets. International Journal of Molecular Sciences. 2014; 15(6):11161-11171.

Chicago/Turabian Style

Zhao, Qiaohui; Liu, Ying; Dong, Wenhua; Zhu, Shiping; Huo, Yongjiu; Wu, Shenglong; Bao, Wenbin. 2014. "Genetic Variations of TAP1 Gene Exon 3 Affects Gene Expression and Escherichia coli F18 Resistance in Piglets." Int. J. Mol. Sci. 15, no. 6: 11161-11171.


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