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Int. J. Mol. Sci. 2014, 15(6), 10855-10867; doi:10.3390/ijms150610855
Article

Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits

1,†
, 2,†
, 1,* , 3
, 1
 and 1
Received: 3 April 2014; in revised form: 22 May 2014 / Accepted: 3 June 2014 / Published: 16 June 2014
(This article belongs to the Section Molecular Pathology)
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Abstract: To improve the efficacy and safety of dural repair in neurosurgical procedures, a new dural material derived from bacterial cellulose (BC) was evaluated in a rabbit model with dural defects. We prepared artificial dura mater using bacterial cellulose which was incubated and fermented from Acetobacter xylinum. The dural defects of the rabbit model were repaired with BC membranes. All surgeries were performed under sodium pentobarbital anesthesia, and all efforts were made to minimize suffering. All animals were humanely euthanized by intravenous injection of phenobarbitone, at each time point, after the operation. Then, the histocompatibility and inflammatory effects of BC were examined by histological examination, real-time fluorescent quantitative polymerase chain reaction (PCR) and Western Blot. BC membranes evenly covered the surface of brain without adhesion. There were seldom inflammatory cells surrounding the membrane during the early postoperative period. The expression of inflammatory cytokines IL-1β, IL-6 and TNF-α as well as iNOS and COX-2 were lower in the BC group compared to the control group at 7, 14 and 21 days after implantation. BC can repair dural defects in rabbit and has a decreased inflammatory response compared to traditional materials. However, the long-term effects need to be validated in larger animals.
Keywords: bacterial cellulose; artificial dura mater; histocompatibility; inflammatory cytokines bacterial cellulose; artificial dura mater; histocompatibility; inflammatory cytokines
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Xu, C.; Ma, X.; Chen, S.; Tao, M.; Yuan, L.; Jing, Y. Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits. Int. J. Mol. Sci. 2014, 15, 10855-10867.

AMA Style

Xu C, Ma X, Chen S, Tao M, Yuan L, Jing Y. Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits. International Journal of Molecular Sciences. 2014; 15(6):10855-10867.

Chicago/Turabian Style

Xu, Chen; Ma, Xia; Chen, Shiwen; Tao, Meifeng; Yuan, Lutao; Jing, Yao. 2014. "Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits." Int. J. Mol. Sci. 15, no. 6: 10855-10867.



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