Next Article in Journal
Proteomic Analysis of Embryogenesis and the Acquisition of Seed Dormancy in Norway Maple (Acer platanoides L.)
Previous Article in Journal
Comparisons of Non-Gaussian Statistical Models in DNA Methylation Analysis
Int. J. Mol. Sci. 2014, 15(6), 10855-10867; doi:10.3390/ijms150610855

Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits

1,* , 3
1 Department of Neurosurgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Yishan Road 600, Shanghai 200233, China 2 School of Perfume and Aroma Technology, Shanghai Institute of Technology, Haiquan Road 100, Shanghai 201418, China 3 School of Life Sciences and Biotechnology, Shanghai Jiaotong University, Dongchuan Road 800, Shanghai 200240, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 3 April 2014 / Revised: 22 May 2014 / Accepted: 3 June 2014 / Published: 16 June 2014
(This article belongs to the Section Molecular Pathology)
View Full-Text   |   Download PDF [1942 KB, uploaded 19 June 2014]   |   Browse Figures


To improve the efficacy and safety of dural repair in neurosurgical procedures, a new dural material derived from bacterial cellulose (BC) was evaluated in a rabbit model with dural defects. We prepared artificial dura mater using bacterial cellulose which was incubated and fermented from Acetobacter xylinum. The dural defects of the rabbit model were repaired with BC membranes. All surgeries were performed under sodium pentobarbital anesthesia, and all efforts were made to minimize suffering. All animals were humanely euthanized by intravenous injection of phenobarbitone, at each time point, after the operation. Then, the histocompatibility and inflammatory effects of BC were examined by histological examination, real-time fluorescent quantitative polymerase chain reaction (PCR) and Western Blot. BC membranes evenly covered the surface of brain without adhesion. There were seldom inflammatory cells surrounding the membrane during the early postoperative period. The expression of inflammatory cytokines IL-1β, IL-6 and TNF-α as well as iNOS and COX-2 were lower in the BC group compared to the control group at 7, 14 and 21 days after implantation. BC can repair dural defects in rabbit and has a decreased inflammatory response compared to traditional materials. However, the long-term effects need to be validated in larger animals.
Keywords: bacterial cellulose; artificial dura mater; histocompatibility; inflammatory cytokines bacterial cellulose; artificial dura mater; histocompatibility; inflammatory cytokines
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Xu, C.; Ma, X.; Chen, S.; Tao, M.; Yuan, L.; Jing, Y. Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits. Int. J. Mol. Sci. 2014, 15, 10855-10867.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert