Next Article in Journal
Cytotoxic Effects of Dillapiole on Embryonic Development of Mouse Blastocysts in Vitro and in Vivo
Next Article in Special Issue
Physical Exercise Promotes Recovery of Neurological Function after Ischemic Stroke in Rats
Previous Article in Journal
Weighted Risk Score-Based Multifactor Dimensionality Reduction to Detect Gene-Gene Interactions in Nasopharyngeal Carcinoma
Previous Article in Special Issue
Carriers in Cell-Based Therapies for Neurological Disorders
Int. J. Mol. Sci. 2014, 15(6), 10738-10750; doi:10.3390/ijms150610738
Article

Damage of Neuroblastoma Cell SH-SY5Y Mediated by MPP+ Inhibits Proliferation of T-Cell Leukemia Jurkat by Co-Culture System

,
,
,
,
, *  and *
Received: 3 January 2014; in revised form: 19 May 2014 / Accepted: 3 June 2014 / Published: 13 June 2014
(This article belongs to the Special Issue Neuroprotective Strategies 2014)
View Full-Text   |   Download PDF [814 KB, updated 19 June 2014; original version uploaded 19 June 2014]   |   Browse Figures
Abstract: The adaptive immune system has implications in pathology of Parkinson’s disease (PD). Research data demonstrated that the peripheral CD4+ T-cell population decreased in pathogenesis of PD. The effect of damaged dopaminergic neurons on peripheral T cells of PD is still unknown. In this study, we constructed a neuronal and glial cells co-culture model by using human neuroblastoma cells SH-SY5Y and gliomas cells U87. After the co-culture cells were treated with neurotoxin 1-methyl-4-phenylpyridinium (MPP+) for 24 h, the conditioned media was harvested and used to cultivate T-cell leukemia Jurkat cells for another 24 h. We then analyzed the cell proliferation, cell cycle and necrosis effect of Jurkat cells. The results showed that co-culture medium of SH-SY5Y and U87 cells with MPP+ treatment inhibited the proliferation of Jurkat cells compared to control medium without MPP+, even though the same concentration of MPP+ had very little toxicity to the Jurkat cell. Furthermore, co-culture medium with low concentration of MPP+ (100 µM) arrested Jurkat cells cycle in G2/M phase through increasing cell cycle division 2 (CDC2) and CyclinB1 expression level, whereas co-culture medium with high concentration of MPP+ (500 µM) induced Jurkat cell necrosis through cellular swelling and membrane breakage. Our data implies that damaged dopamine neurons with glial cells can lead to the reduced number or inhibited proliferation activity of peripheral T cells.
Keywords: Parkinson’s disease; co-culture; human T-cell leukemia; cell cycle; necrosis Parkinson’s disease; co-culture; human T-cell leukemia; cell cycle; necrosis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Wang, F.; Awan, U.F.; Wang, Y.; Wang, L.; Qing, H.; Ma, H.; Deng, Y. Damage of Neuroblastoma Cell SH-SY5Y Mediated by MPP+ Inhibits Proliferation of T-Cell Leukemia Jurkat by Co-Culture System. Int. J. Mol. Sci. 2014, 15, 10738-10750.

AMA Style

Wang F, Awan UF, Wang Y, Wang L, Qing H, Ma H, Deng Y. Damage of Neuroblastoma Cell SH-SY5Y Mediated by MPP+ Inhibits Proliferation of T-Cell Leukemia Jurkat by Co-Culture System. International Journal of Molecular Sciences. 2014; 15(6):10738-10750.

Chicago/Turabian Style

Wang, Fuli; Awan, Umer F.; Wang, Yuanyuan; Wang, Luna; Qing, Hong; Ma, Hong; Deng, Yulin. 2014. "Damage of Neuroblastoma Cell SH-SY5Y Mediated by MPP+ Inhibits Proliferation of T-Cell Leukemia Jurkat by Co-Culture System." Int. J. Mol. Sci. 15, no. 6: 10738-10750.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert