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Int. J. Mol. Sci. 2014, 15(6), 10398-10409; doi:10.3390/ijms150610398
Article

The Metallothionein Gene, TaMT3, from Tamarix androssowii Confers Cd2+ Tolerance in Tobacco

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Received: 4 April 2014; in revised form: 16 May 2014 / Accepted: 16 May 2014 / Published: 10 June 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Cadmium (Cd) is a nonessential microelement and low concentration Cd2+ has strong toxicity to plant growth. Plant metallothioneins, a class of low molecular, cystein(Cys)-rich and heavy-metal binding proteins, play an important role in both metal chaperoning and scavenging of reactive oxygen species (ROS) with their large number of cysteine residues and therefore, protect plants from oxidative damage. In this study, a metallothionein gene, TaMT3, isolated from Tamarix androssowii was transformed into tobacco (Nicotiana tobacum) through Agrobacterium-mediated leaf disc method, and correctly expressed under the control of 35S promoter. Under Cd2+ stress, the transgenic tobacco showed significant increases of superoxide dismutase (SOD) activity and chlorophyll concentration, but decreases of peroxidase (POD) activity and malondialdehyde (MDA) accumulation when compared to the non-transgenic tobacco. Vigorous growth of transgenic tobacco was observed at the early development stages, resulting in plant height and fresh weight were significantly larger than those of the non-transgenic tobacco under Cd2+ stress. These results demonstrated that the expression of the exogenous TaMT3 gene increased the ability of ROS cleaning-up, indicating a stronger tolerance to Cd2+ stress.
Keywords: cadmium; genetic transformation; metallothionein; Tamarix androssowii; tobacco cadmium; genetic transformation; metallothionein; Tamarix androssowii; tobacco
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zhou, B.; Yao, W.; Wang, S.; Wang, X.; Jiang, T. The Metallothionein Gene, TaMT3, from Tamarix androssowii Confers Cd2+ Tolerance in Tobacco. Int. J. Mol. Sci. 2014, 15, 10398-10409.

AMA Style

Zhou B, Yao W, Wang S, Wang X, Jiang T. The Metallothionein Gene, TaMT3, from Tamarix androssowii Confers Cd2+ Tolerance in Tobacco. International Journal of Molecular Sciences. 2014; 15(6):10398-10409.

Chicago/Turabian Style

Zhou, Boru; Yao, Wenjing; Wang, Shengji; Wang, Xinwang; Jiang, Tingbo. 2014. "The Metallothionein Gene, TaMT3, from Tamarix androssowii Confers Cd2+ Tolerance in Tobacco." Int. J. Mol. Sci. 15, no. 6: 10398-10409.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert