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Int. J. Mol. Sci. 2014, 15(6), 10005-10017; doi:10.3390/ijms150610005
Article

Elucidation of the Specific Formation of Homo- and Heterodimeric Forms of ThbZIP1 and Its Role in Stress

1,†
, 2,†
, 3
, 1
 and 1,2,*
Received: 8 April 2014; in revised form: 25 April 2014 / Accepted: 21 May 2014 / Published: 4 June 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Protein–protein interactions are important for the molecular understanding of the biological processes of proteins. The dimerization of bZIPs (basic leucine zipper proteins) is involved in modifying binding site specificities, altering dimer stability, and permitting a new set of specific protein-to-protein interactions to occur at the promoter. In the present study, we studied the whether ThbZIP1 form homo- and heterodimers using the yeast two-hybrid method. Five bZIP genes were cloned from Tamarix hispida to investigate their interaction with ThbZIP1. Our results showed that ThbZIP1 can form homodimers with itself, and three out of five bZIPs could interact with the ThbZIP1 protein to form heterodimers. Real-time RT-PCR results suggested that these ThbZIPs can all respond to abiotic stresses and abscisic acid (ABA), and shared very similar expression patterns in response to NaCl, ABA or PEG6000. Subcellular localization studies showed that all ThbZIPs are targeted to the nucleus. Our results showed that ThbZIP1 are dimeric proteins, which can form homo- or heterodimers.
Keywords: bZIP (basic leucine zipper proteins) transcription factors; heterodimerization; protein–protein interaction; two-hybrid analysis; Tamarix hispida bZIP (basic leucine zipper proteins) transcription factors; heterodimerization; protein–protein interaction; two-hybrid analysis; Tamarix hispida
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Nie, X.; Ji, X.; Liu, Y.; Zheng, L.; Wang, Y. Elucidation of the Specific Formation of Homo- and Heterodimeric Forms of ThbZIP1 and Its Role in Stress. Int. J. Mol. Sci. 2014, 15, 10005-10017.

AMA Style

Nie X, Ji X, Liu Y, Zheng L, Wang Y. Elucidation of the Specific Formation of Homo- and Heterodimeric Forms of ThbZIP1 and Its Role in Stress. International Journal of Molecular Sciences. 2014; 15(6):10005-10017.

Chicago/Turabian Style

Nie, Xianguang; Ji, Xiaoyu; Liu, Yujia; Zheng, Lei; Wang, Yucheng. 2014. "Elucidation of the Specific Formation of Homo- and Heterodimeric Forms of ThbZIP1 and Its Role in Stress." Int. J. Mol. Sci. 15, no. 6: 10005-10017.


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