A pH and Redox Dual Responsive 4-Arm Poly(ethylene glycol)-block-poly(disulfide histamine) Copolymer for Non-Viral Gene Transfection in Vitro and in Vivo
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Characterization of 4-Arm PEG-Conjugated Bioreducible Poly(disulfide histamine)
2.2. Biophysical Properties of the Polyplexes of 4-Arm PEG-SSPHIS
2.3. 4-Arm PEG-SSPHIS Induces Efficient Gene Transfection in HepG2 Cancer Cells in Vitro
2.4. Transgene Expression Induced by Polyplexes of 4-Arm PEG-SSPHIS in HepG2 Tumor
3. Experimental Section
3.1. Materials
3.2. Synthesis of 4-Arm PEG-block-poly(disulfide histamine) (4-Arm PEG-SSPHIS) Copolymer
3.3. Chemical and Biophysical Characterization
3.4. Agarose Gel Electrophoresis Assay
3.5. Cell Culture and Gene Transfection in Vitro
3.6. Cytotoxicity Assay of 4-Arm PEG-SSPHIS
3.7. In Vivo Gene Transfection in HepG2 Tumor Bearing in Nude Mice
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
- Author ContributionsKangkang An and Peng Zhao perform synthesis, characterization and experiments. Chao Lin contributes idea of this paper. The authors wrote manuscript, data analysis and result discussions.
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An, K.; Zhao, P.; Lin, C.; Liu, H. A pH and Redox Dual Responsive 4-Arm Poly(ethylene glycol)-block-poly(disulfide histamine) Copolymer for Non-Viral Gene Transfection in Vitro and in Vivo. Int. J. Mol. Sci. 2014, 15, 9067-9081. https://doi.org/10.3390/ijms15059067
An K, Zhao P, Lin C, Liu H. A pH and Redox Dual Responsive 4-Arm Poly(ethylene glycol)-block-poly(disulfide histamine) Copolymer for Non-Viral Gene Transfection in Vitro and in Vivo. International Journal of Molecular Sciences. 2014; 15(5):9067-9081. https://doi.org/10.3390/ijms15059067
Chicago/Turabian StyleAn, Kangkang, Peng Zhao, Chao Lin, and Hongwei Liu. 2014. "A pH and Redox Dual Responsive 4-Arm Poly(ethylene glycol)-block-poly(disulfide histamine) Copolymer for Non-Viral Gene Transfection in Vitro and in Vivo" International Journal of Molecular Sciences 15, no. 5: 9067-9081. https://doi.org/10.3390/ijms15059067