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Int. J. Mol. Sci. 2014, 15(5), 7667-7683; doi:10.3390/ijms15057667
Article

Huperzine A Ameliorates Cognitive Deficits in Streptozotocin-Induced Diabetic Rats

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Received: 24 February 2014; in revised form: 15 April 2014 / Accepted: 17 April 2014 / Published: 5 May 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: The present study was designed to probe the effects of Huperzine A (HupA) on diabetes-associated cognitive decline (DACD) using a streptozotocin (STZ)-injected rat model. Diabetic rats were treated with HupA (0.05 and 0.1 mg/kg) for seven weeks. Memory functions were evaluated by the water maze test. Nissl staining was selected for detecting neuronal loss. Protein and mRNA levels of brain-derived neurotrophic factor (BDNF) were analyzed by ELISA and real-time PCR, respectively. The activities of choline acetylase (ChAT), Acetylcholinesterase (AChE), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), NF-κB p65 unit, TNF-α, IL-1β, IL-6 and caspase-3 were measured using corresponding kits. After seven weeks, diabetic rats exhibited remarkable reductions in: body weight, percentage of time spent in target quadrant, number of times crossing the platform, ChAT and BDNF levels, SOD, GSH-Px and CAT accompanied with increases in neuronal damage, plasma glucose levels, escape latency, mean path length, AChE, MDA level as well as CAT, NF-κB p65 unit, TNF-α, IL-1β, IL-6 and caspase-3 in cerebral cortex and hippocampus. Supplementation with HupA significantly and dose-dependently reversed the corresponding values in diabetes. It is concluded that HupA ameliorates DACD via modulating BDNF, oxidative stress, inflammation and apoptosis.
Keywords: huperzine A; diabetes-associated cognitive decline; brain-derived neurotrophic factor; oxidative stress; inflammation; apoptosis huperzine A; diabetes-associated cognitive decline; brain-derived neurotrophic factor; oxidative stress; inflammation; apoptosis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Mao, X.-Y.; Cao, D.-F.; Li, X.; Yin, J.-Y.; Wang, Z.-B.; Zhang, Y.; Mao, C.-X.; Zhou, H.-H.; Liu, Z.-Q. Huperzine A Ameliorates Cognitive Deficits in Streptozotocin-Induced Diabetic Rats. Int. J. Mol. Sci. 2014, 15, 7667-7683.

AMA Style

Mao X-Y, Cao D-F, Li X, Yin J-Y, Wang Z-B, Zhang Y, Mao C-X, Zhou H-H, Liu Z-Q. Huperzine A Ameliorates Cognitive Deficits in Streptozotocin-Induced Diabetic Rats. International Journal of Molecular Sciences. 2014; 15(5):7667-7683.

Chicago/Turabian Style

Mao, Xiao-Yuan; Cao, Dan-Feng; Li, Xi; Yin, Ji-Ye; Wang, Zhi-Bin; Zhang, Ying; Mao, Chen-Xue; Zhou, Hong-Hao; Liu, Zhao-Qian. 2014. "Huperzine A Ameliorates Cognitive Deficits in Streptozotocin-Induced Diabetic Rats." Int. J. Mol. Sci. 15, no. 5: 7667-7683.



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