Int. J. Mol. Sci. 2014, 15(5), 7344-7351; doi:10.3390/ijms15057344
Article

Symbiotic Activity of Pea (Pisum sativum) after Application of Nod Factors under Field Conditions

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Received: 26 February 2014; in revised form: 11 April 2014 / Accepted: 15 April 2014 / Published: 29 April 2014
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Growth and symbiotic activity of legumes are mediated by Nod factors (LCO, lipo-chitooligosaccharides). To assess the effects of application of Nod factors on symbiotic activity and yield of pea, a two-year field experiment was conducted on a Haplic Luvisol developed from loess. Nod factors were isolated from Rhizobium leguminosarum bv. viciae strain GR09. Pea seeds were treated with the Nod factors (10−11 M) or water (control) before planting. Symbiotic activity was evaluated by measurements of nitrogenase activity (acetylene reduction assay), nodule number and mass, and top growth by shoot mass, leaf area, and seed and protein yield. Nod factors generally improved pea yield and nitrogenase activity in the relatively dry growing season 2012, but not in the wet growing season in 2013 due to different weather conditions.
Keywords: nodules; nitrogenase activity; seed yield; protein yield; pea shoots; weather conditions
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MDPI and ACS Style

Siczek, A.; Lipiec, J.; Wielbo, J.; Kidaj, D.; Szarlip, P. Symbiotic Activity of Pea (Pisum sativum) after Application of Nod Factors under Field Conditions. Int. J. Mol. Sci. 2014, 15, 7344-7351.

AMA Style

Siczek A, Lipiec J, Wielbo J, Kidaj D, Szarlip P. Symbiotic Activity of Pea (Pisum sativum) after Application of Nod Factors under Field Conditions. International Journal of Molecular Sciences. 2014; 15(5):7344-7351.

Chicago/Turabian Style

Siczek, Anna; Lipiec, Jerzy; Wielbo, Jerzy; Kidaj, Dominika; Szarlip, Paweł. 2014. "Symbiotic Activity of Pea (Pisum sativum) after Application of Nod Factors under Field Conditions." Int. J. Mol. Sci. 15, no. 5: 7344-7351.

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