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Int. J. Mol. Sci. 2014, 15(4), 6544-6555; doi:10.3390/ijms15046544
Article

Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with Osteosarcomas

1,†
,
2,†
,
1
,
1
 and
1,*
1 Department of Orthopedic Surgery, the Second Hospital Affiliated to Xi'an Jiao Tong University School of Medicine, Xi'an 710004, Shaanxi, China 2 Department of Aesthetic Plastic Surgery, the Second Hospital Affiliated to Xi'an Jiao Tong University School of Medicine, Xi'an 710004, Shaanxi, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 13 March 2014 / Revised: 28 March 2014 / Accepted: 9 April 2014 / Published: 17 April 2014
(This article belongs to the Special Issue Advances in Molecular Oncology 2014)
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Abstract

Aim: To investigate whether the aberrant expression of microRNA (miR)-196a and miR-196b can be used as potential prognostic markers of human osteosarcoma. Methods: Quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis was performed to detect the expression levels of miR-196a and miR-196b in osteosarcoma tissues and patients’ sera. Results: Expression levels of miR-196a and miR-196b in osteosarcoma tissues were both significantly higher than those in noncancerous bone tissues (both p < 0.001), in line with which, the serum levels of the two miRNAs were also markedly upregulated in patients with osteosarcomas compared with healthy controls (both p < 0.001). Then, the elevation of serum miR-196a and miR-196b levels both more frequently occurred in osteosarcoma patients with high tumor grade (p = 0.008 and 0.01, respectively), positive metastasis (p = 0.001 and 0.006, respectively) and recurrence (p = 0.001 and 0.006, respectively). Moreover, high serum miR-196a, high serum miR-196b and conjoined expression of miR-196a/miR-196b were all independent prognostic factors for OS (overall survival) and DFS (disease-free survival) of osteosarcoma patients. Conclusion: Our present data indicate the involvement of miR-196a and miR-196b upregulation in the pathogenesis of osteosarcoma. More importantly, the altered levels of circulating miR-196a and miR-196b might have great potential to serve as novel and non-invasive prognostic factors for this malignancy.
Keywords: osteosarcoma; microRNA-196a; microRNA-196b; serum; clinicopathological features; overall survival; disease-free survival osteosarcoma; microRNA-196a; microRNA-196b; serum; clinicopathological features; overall survival; disease-free survival
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Zhang, C.; Yao, C.; Li, H.; Wang, G.; He, X. Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with Osteosarcomas. Int. J. Mol. Sci. 2014, 15, 6544-6555.

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