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Int. J. Mol. Sci. 2014, 15(4), 6544-6555; doi:10.3390/ijms15046544
Article

Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with Osteosarcomas

1,†
,
2,†
,
1
,
1
 and
1,*
1 Department of Orthopedic Surgery, the Second Hospital Affiliated to Xi'an Jiao Tong University School of Medicine, Xi'an 710004, Shaanxi, China 2 Department of Aesthetic Plastic Surgery, the Second Hospital Affiliated to Xi'an Jiao Tong University School of Medicine, Xi'an 710004, Shaanxi, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 13 March 2014 / Revised: 28 March 2014 / Accepted: 9 April 2014 / Published: 17 April 2014
(This article belongs to the Special Issue Advances in Molecular Oncology 2014)
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Abstract

Aim: To investigate whether the aberrant expression of microRNA (miR)-196a and miR-196b can be used as potential prognostic markers of human osteosarcoma. Methods: Quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis was performed to detect the expression levels of miR-196a and miR-196b in osteosarcoma tissues and patients’ sera. Results: Expression levels of miR-196a and miR-196b in osteosarcoma tissues were both significantly higher than those in noncancerous bone tissues (both p < 0.001), in line with which, the serum levels of the two miRNAs were also markedly upregulated in patients with osteosarcomas compared with healthy controls (both p < 0.001). Then, the elevation of serum miR-196a and miR-196b levels both more frequently occurred in osteosarcoma patients with high tumor grade (p = 0.008 and 0.01, respectively), positive metastasis (p = 0.001 and 0.006, respectively) and recurrence (p = 0.001 and 0.006, respectively). Moreover, high serum miR-196a, high serum miR-196b and conjoined expression of miR-196a/miR-196b were all independent prognostic factors for OS (overall survival) and DFS (disease-free survival) of osteosarcoma patients. Conclusion: Our present data indicate the involvement of miR-196a and miR-196b upregulation in the pathogenesis of osteosarcoma. More importantly, the altered levels of circulating miR-196a and miR-196b might have great potential to serve as novel and non-invasive prognostic factors for this malignancy.
Keywords: osteosarcoma; microRNA-196a; microRNA-196b; serum; clinicopathological features; overall survival; disease-free survival osteosarcoma; microRNA-196a; microRNA-196b; serum; clinicopathological features; overall survival; disease-free survival
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Zhang, C.; Yao, C.; Li, H.; Wang, G.; He, X. Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with Osteosarcomas. Int. J. Mol. Sci. 2014, 15, 6544-6555.

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