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Int. J. Mol. Sci. 2014, 15(4), 5570-5581; doi:10.3390/ijms15045570

Silencing of Ether à Go-Go 1 by shRNA Inhibits Osteosarcoma Growth and Cell Cycle Progression

1,*  and 1,*
1 Department of Orthopaedics, the Affiliated Southeast Hospital of Xiamen University, Zhangzhou 363000, China 2 Department of Thoracic Surgery, the Affiliated Tangdu Hospital of Fourth Military Medical University, Xi'an 710038, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 4 February 2014 / Revised: 18 March 2014 / Accepted: 20 March 2014 / Published: 1 April 2014
(This article belongs to the Special Issue Molecular Bases of Cancer Research)
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Recently, a member of the voltage-dependent potassium channel (Kv) family, the Ether à go-go 1 (Eag1) channel was found to be necessary for cell proliferation, cycle progression and tumorigenesis. However, the therapeutic potential of the Eag1 channel in osteosarcoma remains elusive. In the present study, a recombinant adenovirus harboring shRNA against Eag1 was constructed to silence Eag1 expression in human osteosarcoma MG-63 cells. We observed that Eag1-shRNA inhibited the proliferation and colony formation of MG-63 cells due to the induction of G1 phase arrest. Moreover, in vivo experiments showed that Eag1-shRNA inhibited osteosarcoma growth in a xenograft nude mice model. In addition, selective inhibition of Eag1 significantly decreased the expression levels of cyclin D1 and E. Taken together, our data suggest that the Eag1 channel plays a crucial role in regulating the proliferation and cell cycle of osteosarcoma cells, and represents a new and effective therapeutic target for osteosarcoma.
Keywords: ether à go-go 1; osteosarcoma; shRNA; cell proliferation; cell cycle; cyclin ether à go-go 1; osteosarcoma; shRNA; cell proliferation; cell cycle; cyclin
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Wu, J.; Zhong, D.; Fu, X.; Liu, Q.; Kang, L.; Ding, Z. Silencing of Ether à Go-Go 1 by shRNA Inhibits Osteosarcoma Growth and Cell Cycle Progression. Int. J. Mol. Sci. 2014, 15, 5570-5581.

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