Abstract: Three calixarene (Cal-4) derivatives which separately contain ethylester (1), carboxylic acid (2), and crownether (3) at the lower rim with a common reactive thiol at the upper rim were synthesized and constructed to self-assembled monolayers (SAMs) on Au films. After spectroscopic characterization of the monolayers, surface coverage and orientation of antibody immobilized on the Cal-4 derivative SAMs were studied by surface plasmon resonance (SPR) technique. Experimental results revealed that the antibody could be immobilized on the Cal-4 derivatives spontaneously. The orientation of absorbed antibody on the Cal-4 derivative SAMs is related to the SAM’s dipole moment. The possible orientations of the antibody immobilized on the Cal-4 derivative 1 SAM are lying-on or side-on, while on the Cal-4 derivative 2 and Cal-4 derivative 3 head-on and end-on respectively. These experimental results demonstrate the surface dipole moment of Cal-4 derivative appears to be an important factor to antibody orientation. Cal-4 derivatives are useful in developing site direct protein chips.
Keywords: calixarene; surface plasmon resonance (SPR); Immunoglobulin G (IgG); orientation; dipole moment
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Chen, H.; Liu, F.; Qi, F.; Koh, K.; Wang, K. Fabrication of Calixarene Derivative Monolayers to Control Orientation of Antibody Immobilization. Int. J. Mol. Sci. 2014, 15, 5496-5507.
Chen H, Liu F, Qi F, Koh K, Wang K. Fabrication of Calixarene Derivative Monolayers to Control Orientation of Antibody Immobilization. International Journal of Molecular Sciences. 2014; 15(4):5496-5507.
Chen, Hongxia; Liu, Feng; Qi, Fangjie; Koh, Kwangnak; Wang, Keming. 2014. "Fabrication of Calixarene Derivative Monolayers to Control Orientation of Antibody Immobilization." Int. J. Mol. Sci. 15, no. 4: 5496-5507.