Int. J. Mol. Sci. 2014, 15(3), 5175-5192; doi:10.3390/ijms15035175
Article

Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense

1,2email, 2,3email, 4email, 5email, 6email, 6email, 4,5email, 3,7email and 4,5,* email
Received: 28 January 2014; in revised form: 5 March 2014 / Accepted: 5 March 2014 / Published: 24 March 2014
(This article belongs to the collection Advances in Proteomic Research)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Basal stem rot is a common disease that affects oil palm, causing loss of yield and finally killing the trees. The disease, caused by fungus Ganoderma boninense, devastates thousands of hectares of oil palm plantings in Southeast Asia every year. In the present study, root proteins of healthy oil palm seedlings, and those infected with G. boninense, were analyzed by 2-dimensional gel electrophoresis (2-DE). When the 2-DE profiles were analyzed for proteins, which exhibit consistent significant change of abundance upon infection with G. boninense, 21 passed our screening criteria. Subsequent analyses by mass spectrometry and database search identified caffeoyl-CoA O-methyltransferase, caffeic acid O-methyltransferase, enolase, fructokinase, cysteine synthase, malate dehydrogenase, and ATP synthase as among proteins of which abundances were markedly altered.
Keywords: basal stem rot; Ganoderma boninense; biomarker; oil palm; two-dimensional gel electrophoresis; ESI-TRAP
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MDPI and ACS Style

Al-Obaidi, J.R.; Mohd-Yusuf, Y.; Razali, N.; Jayapalan, J.J.; Tey, C.-C.; Md-Noh, N.; Junit, S.M.; Othman, R.Y.; Hashim, O.H. Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense. Int. J. Mol. Sci. 2014, 15, 5175-5192.

AMA Style

Al-Obaidi JR, Mohd-Yusuf Y, Razali N, Jayapalan JJ, Tey C-C, Md-Noh N, Junit SM, Othman RY, Hashim OH. Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense. International Journal of Molecular Sciences. 2014; 15(3):5175-5192.

Chicago/Turabian Style

Al-Obaidi, Jameel R.; Mohd-Yusuf, Yusmin; Razali, Nurhanani; Jayapalan, Jaime J.; Tey, Chin-Chong; Md-Noh, Normahnani; Junit, Sarni M.; Othman, Rofina Y.; Hashim, Onn H. 2014. "Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense." Int. J. Mol. Sci. 15, no. 3: 5175-5192.


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