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Int. J. Mol. Sci. 2014, 15(3), 5175-5192; doi:10.3390/ijms15035175
Article

Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense

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1 Agro-Biotechnology Malaysia Institutes, c/o MARDI Headquarters, Serdang 43400, Selangor, Malaysia 2 Centre for Foundation Studies in Science, University of Malaya, Kuala Lumpur 50603, Malaysia 3 Centre for Research in Biotechnology for Agriculture, University of Malaya, Kuala Lumpur 50603, Malaysia 4 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia 5 University of Malaya Centre for Proteomics Research (UMCPR), University of Malaya, Kuala Lumpur 50603, Malaysia 6 Plant Protection Unit, Sime Darby Sdn Bhd, Km 10, Jalan Banting Kelanang, P.O. Box 207, Banting 42100, Selangor, Malaysia 7 Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
* Author to whom correspondence should be addressed.
Received: 28 January 2014 / Revised: 5 March 2014 / Accepted: 5 March 2014 / Published: 24 March 2014
(This article belongs to the collection Advances in Proteomic Research)
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Abstract

Basal stem rot is a common disease that affects oil palm, causing loss of yield and finally killing the trees. The disease, caused by fungus Ganoderma boninense, devastates thousands of hectares of oil palm plantings in Southeast Asia every year. In the present study, root proteins of healthy oil palm seedlings, and those infected with G. boninense, were analyzed by 2-dimensional gel electrophoresis (2-DE). When the 2-DE profiles were analyzed for proteins, which exhibit consistent significant change of abundance upon infection with G. boninense, 21 passed our screening criteria. Subsequent analyses by mass spectrometry and database search identified caffeoyl-CoA O-methyltransferase, caffeic acid O-methyltransferase, enolase, fructokinase, cysteine synthase, malate dehydrogenase, and ATP synthase as among proteins of which abundances were markedly altered.
Keywords: basal stem rot; Ganoderma boninense; biomarker; oil palm; two-dimensional gel electrophoresis; ESI-TRAP basal stem rot; Ganoderma boninense; biomarker; oil palm; two-dimensional gel electrophoresis; ESI-TRAP
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Al-Obaidi, J.R.; Mohd-Yusuf, Y.; Razali, N.; Jayapalan, J.J.; Tey, C.-C.; Md-Noh, N.; Junit, S.M.; Othman, R.Y.; Hashim, O.H. Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense. Int. J. Mol. Sci. 2014, 15, 5175-5192.

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