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Int. J. Mol. Sci. 2014, 15(3), 3389-3402; doi:10.3390/ijms15033389

Improved Establishment of Embryonic Stem (ES) Cell Lines from the Chinese Kunming Mice by Hybridization with 129 Mice

1
College of Veterinary Medicine, Sichuan Agricultural University, Xinkang Road 46#, Yucheng District, Ya'an 625014, Sichuan, China
2
Life Science College, North-West University, Xi'an 710069, Shaanxi, China
3
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China
*
Author to whom correspondence should be addressed.
Received: 23 October 2013 / Revised: 10 February 2014 / Accepted: 14 February 2014 / Published: 25 February 2014
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Abstract

Chinese Kunming mice (Mus musculus Km), widely used as laboratory animals throughout China, remain very refractory for embryonic stem (ES) cell isolation. The present study was aimed to evaluate the effects of hybridization with 129/Sv mice, and culture media containing fetal bovine serum (FBS) or Knockout serum replacement (KSR) on ES cell isolation from Kunming mice. The results demonstrated that ES cells had been effectively isolated from the hybrid embryos of Kunming and 129/Sv mice using all three media containing 15% FBS, 15% KSR and their mixture of 14% KSR and 1% FBS, individually. These isolated ES cells had maintained in vitro undifferentiated for a long time, exhibiting all features specific for mouse ES cells. In addition, the rates of ES cell isolation in the medium containing 14% KSR and 1% FBS, was 46.67% and significantly higher than those in another two media containing only FBS or KSR (p < 0.05). Contrarily, no ES cell line had been established from Kunming mouse inbred embryos using the same protocols. These results suggested that ES cells with long-term self-renewal ability could be efficiently generated from hybrid embryos of Kunming and 129/Sv mice, and a small volume of FBS was necessary to isolate ES cells in the KSR medium when embryos and early ES cells cultured. View Full-Text
Keywords: Chinese Kunming mouse (Mus musculus Km); 129/Sv mouse; hybrid embryos; embryonic stem (ES) cells Chinese Kunming mouse (Mus musculus Km); 129/Sv mouse; hybrid embryos; embryonic stem (ES) cells
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Yu, S.; Yan, X.; Liu, H.; Cai, X.; Cao, S.; Shen, L.; Zuo, Z.; Deng, J.; Ma, X.; Wang, Y.; Ren, Z. Improved Establishment of Embryonic Stem (ES) Cell Lines from the Chinese Kunming Mice by Hybridization with 129 Mice. Int. J. Mol. Sci. 2014, 15, 3389-3402.

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