Int. J. Mol. Sci. 2014, 15(2), 3319-3335; doi:10.3390/ijms15023319
Article

The Glutathione Peroxidase Gene Family in Thellungiella salsuginea: Genome-Wide Identification, Classification, and Gene and Protein Expression Analysis under Stress Conditions

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Received: 22 January 2014; in revised form: 17 February 2014 / Accepted: 17 February 2014 / Published: 21 February 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Glutathione peroxidases (GPX) catalyze the reduction of H2O2 or organic hydroperoxides to water or corresponding alcohols using reduced glutathione, which plays an essential role in ROS (reactive oxygen species) homeostasis and stress signaling. Thellungiella salsuginea (Eutrema salsugineum), a relative of Arabidopsis thaliana, displays an extremely high level of tolerance to salt, drought, cold and oxidative stresses. The enzymatic antioxidant systems may contribute to the stress tolerance of T. salsuginea. In the present study, we aimed at understanding the roles of the antioxidant enzymes in T. salsuginea by focusing on the GPX family. We identified the eight GPX genes in T. salsuginea, and the structure of the N-terminal domains indicated their putative chloroplastic, mitochondrial and cytoplasmic location. The exon-intron organization of these genes exhibited a conserved pattern among plant GPX genes. Multiple environmental stresses and hormone response related cis-acting elements were predicted in the promoters of TsGPX genes. The gene and protein expression profiles of TsGPXs in response to high level of salinity and osmotic stresses, in leaves and roots of T. salsuginea were investigated using real-time RT-PCR and western blotting analysis. Our result showed that different members of the GPX gene family were coordinately regulated under specific environmental stress conditions, and supported the important roles of TsGPXs in salt and drought stress response in T. salsuginea.
Keywords: Thellungiella salsuginea; glutathione peroxidase; salt stress; drought stress; gene family
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MDPI and ACS Style

Gao, F.; Chen, J.; Ma, T.; Li, H.; Wang, N.; Li, Z.; Zhang, Z.; Zhou, Y. The Glutathione Peroxidase Gene Family in Thellungiella salsuginea: Genome-Wide Identification, Classification, and Gene and Protein Expression Analysis under Stress Conditions. Int. J. Mol. Sci. 2014, 15, 3319-3335.

AMA Style

Gao F, Chen J, Ma T, Li H, Wang N, Li Z, Zhang Z, Zhou Y. The Glutathione Peroxidase Gene Family in Thellungiella salsuginea: Genome-Wide Identification, Classification, and Gene and Protein Expression Analysis under Stress Conditions. International Journal of Molecular Sciences. 2014; 15(2):3319-3335.

Chicago/Turabian Style

Gao, Fei; Chen, Jing; Ma, Tingting; Li, Huayun; Wang, Ning; Li, Zhanglei; Zhang, Zichen; Zhou, Yijun. 2014. "The Glutathione Peroxidase Gene Family in Thellungiella salsuginea: Genome-Wide Identification, Classification, and Gene and Protein Expression Analysis under Stress Conditions." Int. J. Mol. Sci. 15, no. 2: 3319-3335.

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