Next Article in Journal
Rationalization of Activity Cliffs of a Sulfonamide Inhibitor of DNA Methyltransferases with Induced-Fit Docking
Next Article in Special Issue
Non-Specific Inhibition of Ischemia- and Acidosis-Induced Intracellular Calcium Elevations and Membrane Currents by α-Phenyl-N-tert-butylnitrone, Butylated Hydroxytoluene and Trolox
Previous Article in Journal
Prediction of Protein–Protein Interaction with Pairwise Kernel Support Vector Machine
Previous Article in Special Issue
Use of Genetically Modified Mesenchymal Stem Cells to Treat Neurodegenerative Diseases
Article Menu

Export Article

Int. J. Mol. Sci. 2014, 15(2), 3234-3252; doi:10.3390/ijms15023234

The Neuroprotective Role of Acupuncture and Activation of the BDNF Signaling Pathway
Dong Lin 1, Ike De La Pena 2, Lili Lin 1, Shu-Feng Zhou 3,*, Cesar V. Borlongan 2 and Chuanhai Cao 3,*
College of acupuncture, Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou 350122, Fujian, China
Department of Neurosurgery and Brain Repair, College of Medicine, University of South Florida, Tampa, FL 33612, USA
College of pharmacy, Department of pharmaceutical Sciences, University of South Florida, Tampa, FL 33612, USA
Authors to whom correspondence should be addressed; Tel.: +1-813-974-6276 (S.-F.Z.); +1-813-368-0309 (C.C.); Fax: +1-813-905-9885 (C.C.).
Received: 28 November 2013; in revised form: 8 February 2014 / Accepted: 10 February 2014 / Published: 21 February 2014


: Recent studies have been conducted to examine the neuroprotective effects of acupuncture in many neurological disorders. Although the neuroprotective effects of acupuncture has been linked to changes in signaling pathways, accumulating evidence suggest the participation of endogenous biological mediators, such as the neurotrophin (NT) family of proteins, specifically, the brain derived neurotrophic factor (BDNF). Accordingly, acupuncture can inhibit neurodegeneration via expression and activation of BDNF. Moreover, recent studies have reported that acupuncture can increase ATP levels at local stimulated points. We have also demonstrated that acupuncture could activate monocytes and increase the expression of BDNF via the stimulation of ATP. The purpose of this article is to review the recent findings and ongoing studies on the neuroprotective roles of acupuncture and therapeutic implications of acupuncture-induced activation of BDNF and its signaling pathway.
acupuncture; brain derived neurotrophic factor; neuroprotective; cyclophilin A

1. Introduction

Acupuncture, which has been used clinically for more than 2500 years in East Asia, has been reported as an effective treatment approach in various kinds of neurological diseases including movement disorders, Parkinson’s disease (PD), and Alzheimer’s disease (AD) [1]. In the theory of Traditional Chinese medicine (TCM), the function of the acupuncture is related to insertion of needles at specific points of the body, referred to as “acupoints”. Insertion of needles at specific acupoints produces functional specificity [2,3]. Recent clinical studies have shown therapeutic benefits of manual acupuncture or electroacupuncture (EA) in the central nervous system (CNS) [4]. Regardless of the method, the needles inserted into acupoints produce special physical stimulation that can facilitate homeostasis.

Moreover, several recent studies have demonstrated neuroprotective activities of acupuncture therapy. A majority of these studies have proposed that acupuncture exerts its therapeutic effects via regulation of various signaling pathways. For instance, it has been found that acupuncture-mediated neuroprotection can decrease p38MAPK levels and reduce the expression of pro-inflammatory cytokines/mediators and pro nerve growth factor (NGF) [57], which are involved in apoptotic cell death of neurons and oligodendrocytes [811]. In contrast, there are but a few studies which implicated the role of neurotrophins (e.g., BDNF, GDNF) in acupuncture-induced neuroprotection. Brain-derived neurotrophic factor (BDNF), a neurotrophin that regulates the development, regeneration, survival and maintenance of neurons, plays pivotal roles in many aspects of brain function [12]. The most recent data on the correlation between neurotrophins and acupuncture have shown that EA may relieve certain neuropathological disorders by modulating BDNF and its signaling pathway. The beneficial effects of acupuncture have been associated with the release of neuropeptides from nerve endings and modulation of the expression of neurotrophins [13,14]. The purpose of this article is to review the recent findings and ongoing studies on the neuroprotective roles of acupuncture and therapeutic implications of acupuncture-induced activation of BDNF and its signaling pathway.

2. Several Kinds of Physical Stimulation Can Activate the BDNF Signaling Pathway

To date, it is widely accepted that different forms of sensory stimulation induce changes in BDNF expression in relevant CNS structures (e.g., light-induced changes in BDNF levels in the visual cortex) [15]. Previous works also demonstrated that physical exercise and environmental enrichment, or dietary restriction, can induce BDNF expression in the brain [16]. Some data revealed that cellular stress resistance induced by physical and mental exercise could activate several kinases and transcription factors that induce the expression of genes which encode proteins that promote cell survival and synaptic plasticity (e.g., BDNF).

Repetitive transcranial magnetic stimulation (rTMS) has been known to change the expression of BDNF. Some researchers demonstrated that rTMS can upregulate neurotrophic/growth factors in 6-hydroxydopamine (OHDA)-lesioned PD condition, and that BDNF is one of the most important neuroprotective proteins expressed after rTMS [17]. Furthermore, high-frequency rTMS can increase in vivo BDNF levels in patients with neuropsychiatric disorders. Therefore, the neurotrophic factors induced by rTMS, such as BDNF, may modulate the nigrostriatal DA system, and lead to functional recovery [18,19].

Some researchers have also shown that other kinds of stimulation, such as physical exercise, can enhance neuroprotection. Rats subjected to cerebral ischemia reperfusion (IR) injury showed motor function recovery after physical exercise which exerted neuroprotective activities [20,21]. Other studies have shown that rotarod exercise combined with S-nitrosoglutathione (GSNO) can stimulate the expression of neurorepair mediators, such as BDNF and its receptor, tropomyosin receptor kinase B (TrkB) in a stroke rat model induced by middle cerebral artery occlusion. These studies imply that BDNF is required for functional recovery following stroke, because other findings have shown that the beneficial effects of rehabilitation on recovery can be negated by treatment with antisense BDNF oligonucleotide [22,23]. Furthermore, some evidence indicated that treatment with phosphoinositide-13 (PI3) kinase inhibitor can reverse the beneficial effects of exercise in neurorepair. This means that the neuroprotective effects of BDNF may be linked with the activation of the PI3 kinase/Akt pathway to accelerate the recovery of neurological exercise and activate phosphatidylinositol [24].

Meanwhile, many types of electrical stimulation (ES) devices have been shown to promote the survival of degenerated neural cells via increasing the levels of BDNF proteins [25]. A growing amount of experimental evidence suggests that ES could not only directly upregulate the transcriptional induction of BDNF in glial cells, but also induce the production of endogenous BDNF from Müller cells. Accordingly, BDNF may inversely regulate the anti-inflammatory response by attenuating microglial activation [26,27]. In addition, other evidence demonstrated that anti-BDNF neutralizing antibody significantly inhibited the activity of Müller cell to rescue light-damaged photoreceptors. Thus, BDNF might act as an important molecule that could facilitate the survival of light-exposed photoreceptor cells via ES. As for its mechanism, some researchers believe that BDNF-mediated cell survival may be attributed to two types of transmembrane glycoproteins, the high-affinity tyrosine kinase receptors (Trk) and the low-affinity neurotrophin receptor p75 (p75NTR) [28].

3. The Neuroprotective Roles of BDNF/TrkB Signaling

BDNF protein is widely distributed in the neuronal cell bodies, axons and dendrites of the CNS, and is also widely involved in neural plasticity important for learning and memory [29,30]. It is well known that some neuronal activities such as electrical-evoked stimulation also regulate the transport of BDNF mRNA and proteins into dendrites. These mechanisms are considered to be responsible for the ability of locally translated BDNF to modulate synaptic transmission and synaptogenesis [31]. Moreover, BDNF can act via autocrine and paracrine mechanisms, depending on the site of cell surface receptors through which it signals [32]. The binding of BDNF to TrkB can initiate various intracellular signaling pathways, including mitogen activated protein kinase/extracellular signal-regulated protein kinase (MAPK/ERK), phospholipase Cg (PLCγ), and phosphoinositide 3-kinase (PI3K) pathways [33,34]. Several reports showed that BDNF prevented cell death caused by DNA damage by activating the ERK pathway in cortical neurons and cerebellar neurons. Other research reports indicated that the protective effect of BDNF against apoptosis not only requires the activation of the PI-3K/Akt, but also activate ERK pathways through inhibition of GSK-3β and activation of cAMP response element-binding protein (CREB). It has been observed that BDNF also can protect cortical neurons against camptothecin- or serum deprivation-induced apoptosis through activation of ERK and PI3 kinase pathways [35]. As for the mechanism of BDNF-induced protection against neuronal apoptosis, some research have demonstrated that BDNF prevents neuronal death caused by N-methyl-D-aspartate receptor (NMDAR) blockade in corticostriatal organotypic cultures, and its effects are dependent on stimulation of the ERK and PI-3K/Akt signaling cascades [36].

4. The Neuroprotective Effects of Acupuncture in Brain Function

The beneficial effects of acupuncture have been largely attributed to sensory stimulation [37]. Needle insertion into the skin and deeper tissues results in particular patterns of afferent activity in peripheral nerves. The inserted needles are stimulated by manual rotation or through the application of ES, generally referred to as EA [38]. Identification of β-endorphin as the factor mediating the pain-relieving effects of acupuncture, the central mechanism of acupuncture, represents a milestone in the history of acupuncture research [39]. However, many other molecules and systems may also be stimulated by acupuncture resulting in a number of biological effects, such as modulation of stress, pain, autonomic activity and immune systems.

Research progress in the last 20 years has shown that it is possible to affect synthesis of BDNF contributing to the restoration of normal systemic balance through acupuncture. Currently, acupuncture is a relevant therapy in complementary and alternative medicine, and it is believed to release and influence the action of several neurotransmitters (e.g., glutamate, acetylcholine, GABA, and serotonin), and neuropeptides in both CNS and peripheral nervous systems (PNS), respectively [3,40,41].

Scientists have tried to understand how puncture at certain acupoints can affect the distant and complicated system such as the CNS [42]. Previous studies suggested that acupuncture acted as a neuromodulating input into the CNS [43]. Recent research showed that acupunctural signal could travel to the CNS via afferent nerve pathways and cause various neurological and physiological changes [44]. For example, Choi et al. suggested that acupuncture stimulation is responsible for the protection of neurons by accelerating cerebral blood flow and also increasing plasma osmolality and extracellular glutamate in diabetic rats under ischemic conditions [7]. Other research showed that acupuncture can inhibit the nigrostriatal neurodegeneration caused by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) intoxication by increasing expression levels of tyrosine hydroxylase (TH) and dopamine transporter (DAT). Moreover, acupuncture at the GB34 and LR3 acupoints, compared with non-acupoints in the hip, in an MPTP model led to significantly higher levels of TH cells in the striatal and substantia nigra pars compacta regions in mouse brain tissue [5]. Acupuncture treatments in animal experiments have generated valuable mechanistic insights into the pathology of PD and have provided evidence that acupuncture therapy is neuroprotective and can increase various neuroprotective agents such as BDNF, GDNF, and cyclophilin A (CypA) [45].

5. Acupuncture Therapy and BDNF Signaling Pathway in Various Disease States

As discussed above, acupuncture can serve as important therapeutic intervention due to its multiple neuromodulatory functions in the CNS. Recently, accumulating data (Table 1) have shown that acupuncture exerts several beneficial effects in the CNS via activation of BDNF and its down-stream signaling pathway [46,47].

5.1. Depression

Manual and EA stimulation at zusanli (ST36) produced a variety of neuromodulatory functions in the CNS, especially in the hippocampus of rats exposed to immobilization stress. EA stimulation can notably augment the expression of BDNF mRNA, and also BDNF mRNA levels in the hippocampus of AD patients [48]. As for the mechanism of acupuncture in depression, it was reported that EA and manual acupuncture can upregulate expression of hippocampus BDNF protein and mRNA as well as its receptor TrkB. Furthermore, this effect may be explained by activation of adenyl cyclase-cAMP-PKA-CREB signaling pathway [49]. Acupuncture at “Baihui” (GV 20) and “Yintang” (EX-HN 3) can effectively reverse chronic stress-induced down-regulation of BDNF mRNA and protein expression in the frontal cortex and hippocampus of rat models of depression, contributing to its anti-depressive-like actions by protecting neuronal regeneration [50].

5.2. Cerebral Ischemia Injury

EA treatment has the ability to relieve cerebral ischemia-reperfusion injuries and provide neuroprotection by interfering with the expression of multiple apoptosis-related genes, such as c-fos, heat shock protein 70 (HSP70), and even the neurotrophin (NT) family of proteins [5153]. For example, acupuncture can exert beneficial effects in the CNS by enhancing BDNF mRNA expression levels in the hippocampus of mice following cerebral ischemia-reperfusion injury, which protects neurons from injuries, and inhibits apoptosis of hippocampal cells. At the same time, EA treatment at the acupoint of Baihui GV(20) significantly increased expression of BDNF and TrkB and improved motor recovery [54]. Some researchers believe that acupuncture can exert neuroprotective function in ischemic stroke via activation of the PI3K/Akt pathway [55]. Furthermore, EA pretreatment can enhance the tolerance to focal cerebral ischemia, via upregulation of BDNF and the chemokine, stromal cell-derived factor 1α (SDF-1α). Kim et al. demonstrated that pretreatment with EA at both Baihui (GV20) and Dazhui (GV14) acupoints for 20 min increased brain BDNF levels and upregulated the production of SDF-1α in the plasma [56]. Furthermore, some researchers indicated that EA improved neurological deficit and increase the neuroprotective activation of the PI3K/Akt pathway in the ischemic brain [57,58]. Consequently, EA-induced activation of the PI3K/Akt pathway resulted in inhibition of cerebral cell apoptosis. Moreover, EA can also increase the serum BDNF and GDNF levels, activate the PI3K pathway, and enhance the expression of anti-apoptotic signals, like Bcl-2/Bax ratio, in ischemic cerebrum. Our recent findings suggest that EA at acupoints of Quchi and Zusanli exerts neuroprotective function in ischemic stroke via activation of the PI3K/Akt pathway.

5.3. Memory-Deficits

Recent studies suggest that both manual acupuncture and EA can significantly improve learning memory in immobilization or chronic injection of corticosterone (CORT) rat stress models [59,60]. Furthermore, stimulation at the HT7 acupoint alone can increase BDNF mRNA and CREB in the hippocampus compared with stimulation at the Waiguan (TE5) [61]. This indicates that acupuncture can ameliorate learning memory deficits via direct activation of the neurotrophin signaling pathways, significantly reversing CORT or stress induced reduction of BDNF and CREB expression in the hippocampus. Some researchers argue that stimulation at certain acupoints can alleviate memory impairment via the regulation of BDNF resulting to neuronal cell survival and protection against neurodegeneration. Meanwhile, acupuncture can also upregulate phosphorylated CREB, which in turn, promotes BDNF expression to enhance memory [62]. Altogether, these studies suggest that acupuncture can initiate pCREB-BDNF transcription, and modulate adaptive neuronal responses required for learning and memory functions [63].

5.4. Inflammation

The anti-inflammatory effect of BDNF in acupuncture is mainly related to activation of the opioid and nonopioid neurotransmitters. Recent research has demonstrated that acupuncture can decrease the expression of neurotrophins (such as NGF, BDNF, and NT-3) which contribute to hypersensitivity, although it also enhances and prolongs inflammatory response. However, it has also been reported that acupuncture can down-regulate certain proinflammatory neuropeptides and neurotrophins (including BDNF and NGF) in allergic inflammation disease, and can improve clinical signs and symptoms, such as reduction in sneezing, nasal itching, rhinorrhea, and nasal congestion [64,65]. Furthermore, from this result, acupuncture might exert anti-inflammatory actions in allergic rhinitis, and not in the brain, which means the effect of acupuncture can exert different roles in different tissues, even in different pathological processes.

5.5. Others

Acupuncture or ear acupressure has also been shown to protect patients from blindness, and the potential mechanism may be related with the neuroprotection via regulation of NGF and BDNF and their receptors. This results in activation of the survival pathway in contrast to the stimulation of pathways related with apoptosis [66].

6. The Potential Mechanisms of Acupuncture with Regard to Modulation of BDNF

It has been reported that acupuncture can promote the expression of BDNF, and recent studies demonstrate that BDNF can protect neurons both in vitro and in vivo against various insults [35,79]. However, it has just been recently reported that acupuncture can increase the expression of BDNF and CypA in MPTP-induced mouse model of PD [74]. It was suggested that protection of DA neuronal degeneration by acupuncture was due to enhancement of CypA levels. Based on recent published studies, CypA is ubiquitously expressed in the brain [80], and is predominantly localized in neurons [81]. Moreover, although the exact functions of CypA are not yet understood, some studies suggest its contribution in neuronal differentiation and adult cortical plasticity [82,83]. Thus, if we link the increased expression levels of BDNF to CypA following EA, we can hypothesize that CypA activates BDNF and its signaling pathway via acupuncture stimulation (Figure 1). Furthermore, there are recent data which indicate that BDNF treatment induced the expression of CypA in SH-SY5Y cells [74]. Therefore, BDNF may be an important upstream factor in regulating CypA expression during acupuncture treatment. At the same time, acupuncture also plays a critical role in neuroprotection. It may work by triggering an ordered signaling pathway in which BDNF may lie upstream to other endogenous defense systems in order to protect neurons. Based on previous studies, acupuncture can increase BDNF and p-ERK1/2 protein levels in the hippocampus and the prefrontal cortex (PFC) of mice following cerebral ischemia-reperfusion injury [10,56]. In vitro research also confirmed that CypA stimulates ERK1/2 signal pathway in cultured cortical neurons, and protects neurons against in vitro oxidative and ischemic injury via activation ERK1/2 signaling pathway [84]. Collectively, one plausible explanation for the mechanism of acupuncture may be activation of the CypA-ERK pathway to upregulate BDNF expression and improve various neuronal dysfunctions [74].

Furthermore, based on some clinical studies about BDNF functions and the effects of acupuncture, we find that acupuncture can also accelerate neural regeneration in patients with some neurological disorder through neurotrophin-mediated effects. In light of evidence obtained from several animal studies, acupuncture-induced increase in endothelial BDNF exerted neuroprotection after nervous lesion. PD patients who received acupuncture for 3 months showed increased levels of BDNF in peripheral blood. However, in the spinal cord injured patients, BDNF levels were found to be decreased 48 h after acupuncture [77,78,85]. Thus, the potential function of blood BDNF in spinal cord injury remains uncertain. Early data have demonstrated that BDNF is stored in human platelets and released by physical stimulation [85]. However, the platelet number in hunman serum remained constant after acupuncture while BDNF levels decreased, although levels of BDNF-related neurotrophin NGF and other cytokines remained unchanged [78]. Although blood BDNF level has been reported to be associated with the time of acupuncture, inflammation and stress, the underlying mechanism for such relationship is still unclear.

On the other hand, we believe that acupuncture can also increase the expression of BDNF through the activation of monocyte. Based on the research about adenosine A1 receptors and local anti-nociceptive effects of acupuncture [86], acupuncture can increase ATP concentration, even the concentration of adenosine in local tissue. Furthermore, extracellular ATP acts as a stimulus to mediate the recruitment of monocytes in several inflammatory conditions by increasing the production of MCP-1 [87]. We hypothesize that acupuncture can modulate the activation of leucocytes (e.g., monocytes) which are known to respond to environmental simulation. Based on our recent studies, we have found that acupuncture can increase the number of monocytes in peripheral blood mononuclear cells (PBMC) in adult mice (Figure 2). Furthermore, we have cultured J774 cells, a monocyte cell line, in DMEM medium, and treated the cell with ATP at different concentrations (2.53, 2.53 × 10, 2.53 × 102, 2.53 × 103 nM) for 24 h. The results showed that BDNF concentration dose-dependently increased ATP concentration and that BDNF reached peak levels at 4 h. Together, these results demonstrate that ATP can stimulate the expression of BDNF in monocytes. Overall, from the preliminary experiment, we found that monocyte may serve as a bridge connecting acupuncture and the expression of BDNF.

7. The Potential Targets for the Effect of BDNF with Acupuncture in the Future

In view of the above-mentioned data, it is reasonable to suggest that acupuncture can prevent neuronal death and enhance neuronal survival and synaptic plasticity by activating the BDNF signaling pathway. Although the exact mechanism for such relationship is not yet defined, several studies suggest that acupoint selection is an important factor in acupuncture-induced elevation of BDNF. For example, GB34 stimulation, compared to stimulation at ST36, produced more remarkable neuroprotective effects in a PD mouse model. Stimulation at GB34 also enhanced the expression of CypA following activation of BDNF [74]. Moreover, in studies dealing with the effects acupuncture on spinal cord injury, stimulation at the CV 4 produced higher levels of BDNF and TrkB than stimulation at ST28. Focusing on the relationship between special acupoint in special models, there are some indications that acupuncture stimulation at the HT7 acupoint can significantly upregulate the expression of BDNF, than in any other acupoint on a different meridian in either spatial memory-impairment or depression-like behavioral changes model [61,67]. Together, these results indicate highly acupoint specific effects of acupuncture regardless of animal models.

8. Conclusions

According to the philosophical theory in Traditional Chinese medicine (TCM), acupuncture stimulation can activate the specific life energy, which is called “qi”, to regulate various organ or body functional states. BDNF and the neurotrophin family of proteins, which play a critical role in acupuncture-induced neuroprotection, will provide important insights into the nature of acupuncture. Furthermore, the therapeutic strategy of integrating acupuncture with BNDF treatment should serve as a promising treatment method in the future.


This work is supported by the National Natural Science Foundation of China (Grant No. 81273672), and the Natural Science Foundation of Fujian province (Grant No. 2011J0120) is also gratefully acknowledged.

Conflicts of Interest

The authors declare no conflict of interest.


  1. Park, H.J.; Lim, S.; Joo, W.S.; Yin, C.S.; Lee, H.S.; Lee, H.J.; Seo, J.C.; Leem, K.; Son, Y.S.; Kim, Y.J.; et al. Acupuncture prevents 6-hydroxydopamine-induced neuronal death in the nigrostriatal dopaminergic system in the rat parkinson’s disease model. Exp. Neurol 2003, 180, 93–98. [Google Scholar]
  2. Diehl, D.L.; Kaplan, G.; Coulter, I.; Glik, D.; Hurwitz, E.L. Use of acupuncture by american physicians. J. Altern. Complement. Med 1997, 3, 119–126. [Google Scholar]
  3. Chuang, C.M.; Hsieh, C.L.; Li, T.C.; Lin, J.G. Acupuncture stimulation at baihui acupoint reduced cerebral infarct and increased dopamine levels in chronic cerebral hypoperfusion and ischemia-reperfusion injured sprague-dawley rats. Am. J. Chin. Med 2007, 35, 779–791. [Google Scholar]
  4. Donoyama, N.; Ohkoshi, N. Effects of traditional japanese massage therapy on various symptoms in patients with parkinson’s disease: A case-series study. J. Altern. Complement. Med 2012, 18, 294–299. [Google Scholar]
  5. Choi, Y.G.; Yeo, S.; Hong, Y.M.; Lim, S. Neuroprotective changes of striatal degeneration-related gene expression by acupuncture in an mptp mouse model of parkinsonism: Microarray analysis. Cell. Mol. Neurobiol 2011, 31, 377–391. [Google Scholar]
  6. Choi, D.C.; Lee, J.Y.; Moon, Y.J.; Kim, S.W.; Oh, T.H.; Yune, T.Y. Acupuncture-mediated inhibition of inflammation facilitates significant functional recovery after spinal cord injury. Neurobiol. Dis 2010, 39, 272–282. [Google Scholar]
  7. Choi, S.; Lee, G.J.; Chae, S.J.; Kang, S.W.; Yin, C.S.; Lee, S.H.; Choi, S.K.; Park, H.K. Potential neuroprotective effects of acupuncture stimulation on diabetes mellitus in a global ischemic rat model. Physiol. Meas 2010, 31, 633–647. [Google Scholar]
  8. Doo, A.R.; Kim, S.T.; Kim, S.N.; Moon, W.; Yin, C.S.; Chae, Y.; Park, H.K.; Lee, H.; Park, H.J. Neuroprotective effects of bee venom pharmaceutical acupuncture in acute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of parkinson’s disease. Neurol. Res 2010, 32, 88–91. [Google Scholar]
  9. Hong, M.S.; Park, H.K.; Yang, J.S.; Park, H.J.; Kim, S.T.; Kim, S.N.; Park, J.Y.; Song, J.Y.; Jo, D.J.; Park, S.W.; et al. Gene expression profile of acupuncture treatment in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced parkinson’s disease model. Neurol. Res 2010, 32, 74–78. [Google Scholar]
  10. Kim, W.S.; Kim, I.S.; Kim, S.J.; Wei, P.; Hyung Choi, D.; Han, T.R. Effect of electroacupuncture on motor recovery in a rat stroke model during the early recovery stage. Brain Res 2009, 1248, 176–183. [Google Scholar]
  11. Hwang, I.K.; Chung, J.Y.; Yoo, D.Y.; Yi, S.S.; Youn, H.Y.; Seong, J.K.; Yoon, Y.S. Effects of electroacupuncture at zusanli and baihui on brain-derived neurotrophic factor and cyclic amp response element-binding protein in the hippocampal dentate gyrus. J. Vet. Med. Sci 2010, 72, 1431–1436. [Google Scholar]
  12. Abbracchio, M.P.; Burnstock, G.; Verkhratsky, A.; Zimmermann, H. Purinergic signalling in the nervous system: An overview. Trends Neurosci 2009, 32, 19–29. [Google Scholar]
  13. Yang, C.P.; Chang, M.H.; Liu, P.E.; Li, T.C.; Hsieh, C.L.; Hwang, K.L.; Chang, H.H. Acupuncture versus topiramate in chronic migraine prophylaxis: A randomized clinical trial. Cephalalgia 2011, 31, 1510–1521. [Google Scholar]
  14. Fischer, M.; Wille, G.; Klien, S.; Shanib, H.; Holle, D.; Gaul, C.; Broessner, G. Brain-derived neurotrophic factor in primary headaches. J. Headache Pain 2012, 13, 469–475. [Google Scholar]
  15. Castrén, E.; Zafra, F.; Thoenen, H.; Lindholm, D. Light regulates expression of brain-derived neurotrophic factor mrna in rat visual cortex. Proc. Natl. Acad. Sci. USA 1992, 89, 9444–9448. [Google Scholar]
  16. Mattson, M.P.; Duan, W.; Wan, R.; Guo, Z. Prophylactic activation of neuroprotective stress response pathways by dietary and behavioral manipulations. NeuroRx 2004, 1, 111–116. [Google Scholar]
  17. Lee, J.Y.; Kim, S.H.; Ko, A.R.; Lee, J.S.; Yu, J.H.; Seo, J.H.; Cho, B.P.; Cho, S.R. Therapeutic effects of repetitive transcranial magnetic stimulation in an animal model of parkinson’s disease. Brain Res 2013, 1537, 290–302. [Google Scholar]
  18. Yue, L.; Xiao-Lin, H.; Tao, S. The effects of chronic repetitive transcranial magnetic stimulation on glutamate and gamma-aminobutyric acid in rat brain. Brain Res 2009, 1260, 94–99. [Google Scholar]
  19. Yukimasa, T.; Yoshimura, R.; Tamagawa, A.; Uozumi, T.; Shinkai, K.; Ueda, N.; Tsuji, S.; Nakamura, J. High-frequency repetitive transcranial magnetic stimulation improves refractory depression by influencing catecholamine and brain-derived neurotrophic factors. Pharmacopsychiatry 2006, 39, 52–59. [Google Scholar]
  20. Matsuda, F.; Sakakima, H.; Yoshida, Y. The effects of early exercise on brain damage and recovery after focal cerebral infarction in rats. Acta Physiol 2011, 201, 275–287. [Google Scholar]
  21. Ke, Z.; Yip, S.P.; Li, L.; Zheng, X.X.; Tam, W.K.; Tong, K.Y. The effects of voluntary, involuntary, and forced exercises on motor recovery in a stroke rat model. Conf. Proc. IEEE Eng. Med. Biol. Soc 2011, 2011, 8223–8226. [Google Scholar]
  22. Ploughman, M.; Windle, V.; MacLellan, C.L.; White, N.; Doré, J.J.; Corbett, D. Brain-derived neurotrophic factor contributes to recovery of skilled reaching after focal ischemia in rats. Stroke 2009, 40, 1490–1495. [Google Scholar]
  23. Sakakima, H.; Khan, M.; Dhammu, T.S.; Shunmugavel, A.; Yoshida, Y.; Singh, I.; Singh, A.K. Stimulation of functional recovery via the mechanisms of neurorepair by s-nitrosoglutathione and motor exercise in a rat model of transient cerebral ischemia and reperfusion. Restor. Neurol. Neurosci 2012, 30, 383–396. [Google Scholar]
  24. Chen, M.J.; Russo-Neustadt, A.A. Exercise activates the phosphatidylinositol 3-kinase pathway. Brain Res. Mol. Brain Res 2005, 135, 181–193. [Google Scholar]
  25. Ni, Y.Q.; Gan, D.K.; Xu, H.D.; Xu, G.Z.; Da, C.D. Neuroprotective effect of transcorneal electrical stimulation on light-induced photoreceptor degeneration. Exp. Neurol 2009, 219, 439–452. [Google Scholar]
  26. Gomes, J.R.; Costa, J.T.; Melo, C.V.; Felizzi, F.; Monteiro, P.; Pinto, M.J.; Inácio, A.R.; Wieloch, T.; Almeida, R.D.; Grãos, M.; et al. Excitotoxicity downregulates trkb.Fl signaling and upregulates the neuroprotective truncated trkb receptors in cultured hippocampal and striatal neurons. J. Neurosci 2012, 32, 4610–4622. [Google Scholar]
  27. Sato, T.; Fujikado, T.; Morimoto, T.; Matsushita, K.; Harada, T.; Tano, Y. Effect of electrical stimulation on igf-1 transcription by l-type calcium channels in cultured retinal müller cells. Jpn. J. Ophthalmol 2008, 52, 217–223. [Google Scholar]
  28. Zhou, W.T.; Ni, Y.Q.; Jin, Z.B.; Zhang, M.; Wu, J.H.; Zhu, Y.; Xu, G.Z.; Gan, D.K. Electrical stimulation ameliorates light-induced photoreceptor degeneration in vitro via suppressing the proinflammatory effect of microglia and enhancing the neurotrophic potential of müller cells. Exp. Neurol 2012, 238, 192–208. [Google Scholar]
  29. Mowla, S.J.; Farhadi, H.F.; Pareek, S.; Atwal, J.K.; Morris, S.J.; Seidah, N.G.; Murphy, R.A. Biosynthesis and post-translational processing of the precursor to brain-derived neurotrophic factor. J. Biol. Chem 2001, 276, 12660–12666. [Google Scholar]
  30. Lin, E.; Hong, C.J.; Hwang, J.P.; Liou, Y.J.; Yang, C.H.; Cheng, D.; Tsai, S.J. Gene-gene interactions of the brain-derived neurotrophic-factor and neurotrophic tyrosine kinase receptor 2 genes in geriatric depression. Rejuvenation Res 2009, 12, 387–393. [Google Scholar]
  31. Tongiorgi, E. Activity-dependent expression of brain-derived neurotrophic factor in dendrites: Facts and open questions. Neurosci. Res 2008, 61, 335–346. [Google Scholar]
  32. Murer, M.G.; Yan, Q.; Raisman-Vozari, R. Brain-derived neurotrophic factor in the control human brain, and in alzheimer’s disease and parkinson’s disease. Prog. Neurobiol 2001, 63, 71–124. [Google Scholar]
  33. Schäbitz, W.R.; Sommer, C.; Zoder, W.; Kiessling, M.; Schwaninger, M.; Schwab, S. Intravenous brain-derived neurotrophic factor reduces infarct size and counterregulates bax and bcl-2 expression after temporary focal cerebral ischemia. Stroke 2000, 31, 2212–2217. [Google Scholar]
  34. Schlessinger, J. Cell signaling by receptor tyrosine kinases. Cell 2000, 103, 211–225. [Google Scholar]
  35. Hetman, M.; Kanning, K.; Cavanaugh, J.E.; Xia, Z. Neuroprotection by brain-derived neurotrophic factor is mediated by extracellular signal-regulated kinase and phosphatidylinositol 3-kinase. J. Biol. Chem 1999, 274, 22569–22580. [Google Scholar]
  36. Xia, Y.; Wang, C.Z.; Liu, J.; Anastasio, N.C.; Johnson, K.M. Brain-derived neurotrophic factor prevents phencyclidine-induced apoptosis in developing brain by parallel activation of both the erk and pi-3k/akt pathways. Neuropharmacology 2010, 58, 330–336. [Google Scholar]
  37. Andersson, S.; Lundeberg, T. Acupuncture--from empiricism to science: Functional background to acupuncture effects in pain and disease. Med. Hypotheses 1995, 45, 271–281. [Google Scholar]
  38. Zhao, Z.Q. Neural mechanism underlying acupuncture analgesia. Prog. Neurobiol 2008, 85, 355–375. [Google Scholar]
  39. Kaptchuk, T.J. Acupuncture: Theory, efficacy, and practice. Ann. Intern. Med 2002, 136, 374–383. [Google Scholar]
  40. Guan, X.M.; Wang, C.Y.; Liu, X.C.; Zhang, Y.W.; Zheng, X.Y.; Liang, X.C.; Li, L.L. The influence of ach on the metabolism of 5-ht in the brain during acupuncture analgesia. Zhen Ci Yan Jiu 1988, 13, 314–318. [Google Scholar]
  41. Manni, L.; Albanesi, M.; Guaragna, M.; Barbaro Paparo, S.; Aloe, L. Neurotrophins and acupuncture. Auton. Neurosci 2010, 157, 9–17. [Google Scholar]
  42. Yu, Y.P.; Ju, W.P.; Li, Z.G.; Wang, D.Z.; Wang, Y.C.; Xie, A.M. Acupuncture inhibits oxidative stress and rotational behavior in 6-hydroxydopamine lesioned rat. Brain Res 2010, 1336, 58–65. [Google Scholar]
  43. Han, J.S.; Terenius, L. Neurochemical basis of acupuncture analgesia. Annu. Rev. Pharmacol. Toxicol 1982, 22, 193–220. [Google Scholar]
  44. Guo, J.; Liu, J.; Fu, W.; Ma, W.; Xu, Z.; Yuan, M.; Zhou, X.; Hu, J. Effect of electroacupuncture stimulation of hindlimb on seizure incidence and supragranular mossy fiber sprouting in a rat model of epilepsy. J. Physiol. Sci 2008, 58, 309–315. [Google Scholar]
  45. Joh, T.H.; Park, H.J.; Kim, S.N.; Lee, H. Recent development of acupuncture on parkinson’s disease. Neurol. Res 2010, 32, 5–9. [Google Scholar]
  46. Cakmak, Y.O. Epilepsy, electroacupuncture and the nucleus of the solitary tract. Acupunct. Med 2006, 24, 164–168. [Google Scholar]
  47. Chen, Y.; Zhou, J.; Li, J.; Yang, S.B.; Mo, L.Q.; Hu, J.H.; Yuan, W.L. Electroacupuncture pretreatment prevents cognitive impairment induced by limb ischemia-reperfusion via inhibition of microglial activation and attenuation of oxidative stress in rats. Brain Res 2012, 1432, 36–45. [Google Scholar]
  48. Yun, S.J.; Park, H.J.; Yeom, M.J.; Hahm, D.H.; Lee, H.J.; Lee, E.H. Effect of electroacupuncture on the stress-induced changes in brain-derived neurotrophic factor expression in rat hippocampus. Neurosci. Lett 2002, 318, 85–88. [Google Scholar]
  49. Hu, L.; Liang, J.; Jin, S.Y.; Han, Y.J.; Lu, J.; Tu, Y. Progress of researches on mechanisms of acupuncture underlying improvement of depression in the past five years. Zhen Ci Yan Jiu 2013, 38, 253–258. [Google Scholar]
  50. Liang, J.; Lu, J.; Cui, S.F.; Wang, J.R.; Tu, Y. Effect of acupuncture on expression of brain-derived neurotrophic factor gene and protein in frontal cortex and hippocampus of depression rats. Zhen Ci Yan Jiu 2012, 37, 20–24. [Google Scholar]
  51. Zhao, J.X.; Tian, Y.X.; Xiao, H.L.; Hu, M.X.; Chen, W.R. Effects of electroacupuncture on hippocampal and cortical apoptosis in a mouse model of cerebral ischemia-reperfusion injury. J. Tradit. Chin. Med 2011, 31, 349–355. [Google Scholar]
  52. Zhao, J.; Xu, H.; Tian, Y.; Hu, M.; Xiao, H. Effect of electroacupuncture on brain-derived neurotrophic factor mrna expression in mouse hippocampus following cerebral ischemia-reperfusion injury. J. Tradit. Chin. Med 2013, 33, 253–257. [Google Scholar]
  53. Tao, J.; Chen, B.; Gao, Y.; Yang, S.; Huang, J.; Jiang, X.; Wu, Y.; Peng, J.; Hong, Z.; Chen, L. Electroacupuncture enhances hippocampal nscs proliferation in cerebral ischemia-reperfusion injured rats via activation of notch signaling pathway. Int. J. Neurosci 2014, 124, 204–212. [Google Scholar]
  54. Kim, M.W.; Chung, Y.C.; Jung, H.C.; Park, M.S.; Han, Y.M.; Chung, Y.A.; Maeng, L.S.; Park, S.I.; Lim, J.; Im, W.S.; et al. Electroacupuncture enhances motor recovery performance with brain-derived neurotrophic factor expression in rats with cerebral infarction. Acupunct. Med 2012, 30, 222–226. [Google Scholar]
  55. Chen, A.; Lin, Z.; Lan, L.; Xie, G.; Huang, J.; Lin, J.; Peng, J.; Tao, J.; Chen, L. Electroacupuncture at the quchi and zusanli acupoints exerts neuroprotective role in cerebral ischemia-reperfusion injured rats via activation of the pi3k/akt pathway. Int. J. Mol. Med 2012, 30, 791–796. [Google Scholar]
  56. Kim, J.H.; Choi, K.H.; Jang, Y.J.; Kim, H.N.; Bae, S.S.; Choi, B.T.; Shin, H.K. Electroacupuncture preconditioning reduces cerebral ischemic injury via bdnf and sdf-1α in mice. BMC Complement. Altern. Med 2013, 13, 22. [Google Scholar] [CrossRef]
  57. Xia, Y.; Cao, X.; Wu, G.-C.; Cheng, J. Acupuncture Therapy for Neurological Diseases: A Neurobiological View; Springer: Beijing, China, 2010. [Google Scholar]
  58. Zeng, B.-Y.; Zhao, K.; Liang, F. Neurobiology of Acupuncture, 1st ed.; Academic Press: Waltham, MA, USA, 2013. [Google Scholar]
  59. Kim, H.; Park, H.J.; Han, S.M.; Hahm, D.H.; Lee, H.J.; Kim, K.S.; Shim, I. The effects of acupuncture stimulation at pc6 (neiguan) on chronic mild stress-induced biochemical and behavioral responses. Neurosci. Lett 2009, 460, 56–60. [Google Scholar]
  60. Lee, B.; Shim, I.; Lee, H.J.; Yang, Y.; Hahm, D.H. Effects of acupuncture on chronic corticosterone-induced depression-like behavior and expression of neuropeptide y in the rats. Neurosci. Lett 2009, 453, 151–156. [Google Scholar]
  61. Lee, B.; Sur, B.J.; Kwon, S.; Jung, E.; Shim, I.; Lee, H.; Hahm, D.H. Acupuncture stimulation alleviates corticosterone-induced impairments of spatial memory and cholinergic neurons in rats. Evid. Based Complement. Altern. Med 2012, 2012, 670536. [Google Scholar] [CrossRef]
  62. Wang, T.; Liu, C.Z.; Yu, J.C.; Jiang, W.; Han, J.X. Acupuncture protected cerebral multi-infarction rats from memory impairment by regulating the expression of apoptosis related genes bcl-2 and bax in hippocampus. Physiol. Behav 2009, 96, 155–161. [Google Scholar]
  63. Tyler, W.J.; Alonso, M.; Bramham, C.R.; Pozzo-Miller, L.D. From acquisition to consolidation: On the role of brain-derived neurotrophic factor signaling in hippocampal-dependent learning. Learn. Mem 2002, 9, 224–237. [Google Scholar]
  64. Liang, C.; Jiang, T. Acupoint autohemotherapy for allergic rhinitis and its effect on serum il-12 and ifn-gamma. Zhongguo Zhen Jiu 2012, 32, 1077–1080. [Google Scholar]
  65. Xiao, L.; Li, B.; Du, Y.H.; Xiong, J.; Gao, X. Systematic evaluation of the randomized controlled trials about acupuncture and moxibustion treatment of allergic rhinitis. Zhongguo Zhen Jiu 2009, 29, 512–516. [Google Scholar]
  66. Rom, E. Sensory stimulation for lowering intraocular pressure, improving blood flow to the optic nerve and neuroprotection in primary open-angle glaucoma. Acupunct. Med 2013, 31, 416–421. [Google Scholar]
  67. Park, H.; Yoo, D.; Kwon, S.; Yoo, T.W.; Park, H.J.; Hahm, D.H.; Lee, H.; Kim, S.T. Acupuncture stimulation at ht7 alleviates depression-induced behavioral changes via regulation of the serotonin system in the prefrontal cortex of maternally-separated rat pups. J. Physiol. Sci 2012, 62, 351–357. [Google Scholar]
  68. Wang, S.J.; Tan, L.H.; Liu, J.L. Effect of electroacupuncture at different acupoints on expression of cervico-spinal gdnf and bdnf and their receptor genes in neck-incision pain rats. Zhen Ci Yan Jiu 2012, 37, 351–356. [Google Scholar]
  69. Zhang, X.F.; Zou, Y.; Zhao, Y.; Wang, T.H.; Zhang, W. Effects of electroacupuncture of “Governor vessel” Acupoints on changes of bdnf in the cortical motor area of mice with spinal cord transection. Sichuan Da Xue Xue Bao Yi Xue Ban 2012, 43, 250–253. [Google Scholar]
  70. Sun, H.; Zhang, H.; Lin, B.S. Effect of acupuncture on the expression of bcl-xl and bdnf of retina in rabbits with chronic intraocular hypertension. Zhongguo Zhen Jiu 2010, 30, 661–664. [Google Scholar]
  71. Hua, C.; Liu, Q.S. Effects of acupuncture on the expression of brain-derived neurotrophic factor in the ovariectomized rat fracture model. Zhongguo Zhen Jiu 2009, 29, 303–308. [Google Scholar]
  72. Manni, L.; Aloe, L.; Fiore, M. Changes in cognition induced by social isolation in the mouse are restored by electro-acupuncture. Physiol. Behav 2009, 98, 537–542. [Google Scholar]
  73. Wang, J.H.; Chen, B.G.; Yin, J.; Wang, G.; Zou, W.G.; Luo, X.J. Effect of electroacupuncture of different acupoints on the excitability of detrusor muscle and the expression of bdnf and trkb in the spinal cord of rats with urinary retention due to spinal cord injury. Zhen Ci Yan Jiu 2009, 34, 387–392. [Google Scholar]
  74. Jeon, S.; Kim, Y.J.; Kim, S.T.; Moon, W.; Chae, Y.; Kang, M.; Chung, M.Y.; Lee, H.; Hong, M.S.; Chung, J.H.; et al. Proteomic analysis of the neuroprotective mechanisms of acupuncture treatment in a parkinson’s disease mouse model. Proteomics 2008, 8, 4822–4832. [Google Scholar]
  75. Chen, J.; Qi, J.G.; Zhang, W.; Zhou, X.; Meng, Q.S.; Zhang, W.M.; Wang, X.Y.; Wang, T.H. Electro-acupuncture induced ngf, bdnf and nt-3 expression in spared l6 dorsal root ganglion in cats subjected to removal of adjacent ganglia. Neurosci. Res 2007, 59, 399–405. [Google Scholar]
  76. Liang, X.B.; Liu, X.Y.; Li, F.Q.; Luo, Y.; Lu, J.; Zhang, W.M.; Wang, X.M.; Han, J.S. Long-term high-frequency electro-acupuncture stimulation prevents neuronal degeneration and up-regulates bdnf mrna in the substantia nigra and ventral tegmental area following medial forebrain bundle axotomy. Brain Res. Mol. Brain Res 2002, 108, 51–59. [Google Scholar]
  77. Xia, Y.; Wang, H.D.; Ding, Y.; Kang, B.; Liu, W.G. Parkinson’s disease combined with depression treated with electroacupuncture and medication and its effect on serum bdnf. Zhongguo Zhen Jiu 2012, 32, 1071–1074. [Google Scholar]
  78. Moldenhauer, S.; Burgauner, M.; Hellweg, R.; Lun, A.; Hohenböken, M.; Dietz, E.; Kiesewetter, H.; Salama, A.; Moldenhauer, A. Mobilization of cd133(+)cd34(−) cells in healthy individuals following whole-body acupuncture for spinal cord injuries. J. Neurosci.Res 2010, 88, 1645–1650. [Google Scholar]
  79. Mattson, M.P.; Cheng, B.; Smith-Swintosky, V.L. Mechanisms of neurotrophic factor protection against calcium- and free radical-mediated excitotoxic injury: Implications for treating neurodegenerative disorders. Exp. Neurol 1993, 124, 89–95. [Google Scholar]
  80. Ryffel, B.; Woerly, G.; Greiner, B.; Haendler, B.; Mihatsch, M.J.; Foxwell, B.M. Distribution of the cyclosporine binding protein cyclophilin in human tissues. Immunology 1991, 72, 399–404. [Google Scholar]
  81. Göldner, F.M.; Patrick, J.W. Neuronal localization of the cyclophilin a protein in the adult rat brain. J. Comp. Neurol 1996, 372, 283–293. [Google Scholar]
  82. Chiu, R.; Rey, O.; Zheng, J.Q.; Twiss, J.L.; Song, J.; Pang, S.; Yokoyama, K.K. Effects of altered expression and localization of cyclophilin a on differentiation of p19 embryonic carcinoma cells. Cell. Mol. Neurobiol 2003, 23, 929–943. [Google Scholar]
  83. Song, J.; Lu, Y.C.; Yokoyama, K.; Rossi, J.; Chiu, R. Cyclophilin a is required for retinoic acid-induced neuronal differentiation in p19 cells. J. Biol. Chem 2004, 279, 24414–24419. [Google Scholar]
  84. Boulos, S.; Meloni, B.P.; Arthur, P.G.; Majda, B.; Bojarski, C.; Knuckey, N.W. Evidence that intracellular cyclophilin a and cyclophilin a/cd147 receptor-mediated erk1/2 signalling can protect neurons against in vitro oxidative and ischemic injury. Neurobiol. Dis 2007, 25, 54–64. [Google Scholar]
  85. Fujimura, H.; Altar, C.A.; Chen, R.; Nakamura, T.; Nakahashi, T.; Kambayashi, J.; Sun, B.; Tandon, N.N. Brain-derived neurotrophic factor is stored in human platelets and released by agonist stimulation. Thromb. Haemost 2002, 87, 728–734. [Google Scholar]
  86. Goldman, N.; Chen, M.; Fujita, T.; Xu, Q.; Peng, W.; Liu, W.; Jensen, T.K.; Pei, Y.; Wang, F.; Han, X.; et al. Adenosine a1 receptors mediate local anti-nociceptive effects of acupuncture. Nat. Neurosci 2010, 13, 883–888. [Google Scholar]
  87. Fang, K.M.; Wang, Y.L.; Huang, M.C.; Sun, S.H.; Cheng, H.; Tzeng, S.F. Expression of macrophage inflammatory protein-1α and monocyte chemoattractant protein-1 in glioma-infiltrating microglia: Involvement of atp and p2×7 receptor. J. Neurosci. Res 2011, 89, 199–211. [Google Scholar]
Figure 1. Acupuncture can increase the expression of BDNF (Brain-derived neurotrophic factor) via PI3K/Akt (Phosphatidylinositol-4,5-bisphosphate 3-kinase) and MEK/ERK1/2 (extracellular-signal-regulated kinases) signaling pathway by activating the tropomyosin receptor kinase B (TrkB), a high affinity catalytic receptor for several neurotrophins that induce the activation of survival signaling pathway. This ultimately leads to the phosphorylation and activation of the transcription factor CREB (cAMP response element-binding protein) that mediates transcription of BDNF gene expression leading to neuroprotection. In this article, we hypothesize that cyclophilin A (CypA) plays an important role in the activation of BDNF. On the contrary, BDNF may also be an important upstream factor in regulating CypA expression during acupuncture treatment. Thus, the exact mechanism between the effect of acupuncture and the expression of CypA is still unknown.
Figure 1. Acupuncture can increase the expression of BDNF (Brain-derived neurotrophic factor) via PI3K/Akt (Phosphatidylinositol-4,5-bisphosphate 3-kinase) and MEK/ERK1/2 (extracellular-signal-regulated kinases) signaling pathway by activating the tropomyosin receptor kinase B (TrkB), a high affinity catalytic receptor for several neurotrophins that induce the activation of survival signaling pathway. This ultimately leads to the phosphorylation and activation of the transcription factor CREB (cAMP response element-binding protein) that mediates transcription of BDNF gene expression leading to neuroprotection. In this article, we hypothesize that cyclophilin A (CypA) plays an important role in the activation of BDNF. On the contrary, BDNF may also be an important upstream factor in regulating CypA expression during acupuncture treatment. Thus, the exact mechanism between the effect of acupuncture and the expression of CypA is still unknown.
Ijms 15 03234f1 1024
Figure 2. The relationship between monocytes and BDNF expression in acupuncture. (A) cell distribution before acupuncture; (B) cell distribution post acupuncture; (C) is the graph of data from A and B; (D) BDNF expression after stimulation with ATP at different concentration and (E) is the time course expression of BDNF level in J775 cell line stimulated with ATP; The proportion of monocyte was detected by flow cytometry before/after acupuncture. Cluster analysis of monocytes using Forward/Side scatter characteristics. The proportion of monocyte increased from 4.8%–11.8% in PBMC. The granulocyte decreased from 74.4%–53.7%. (D,E) The J774 Cell line was treated by ATP from the concentration of 2.53 nM–2.53 μM. After incubated for 24 h, the supernatant was detected by BDNF ELISA kit at 1, 2, 4, and 24 h. The result showed that ATP can induce the expression of BDNF with a dose-dependent, and reach a peak level at 4 h, and then decreased at 24 h.
Figure 2. The relationship between monocytes and BDNF expression in acupuncture. (A) cell distribution before acupuncture; (B) cell distribution post acupuncture; (C) is the graph of data from A and B; (D) BDNF expression after stimulation with ATP at different concentration and (E) is the time course expression of BDNF level in J775 cell line stimulated with ATP; The proportion of monocyte was detected by flow cytometry before/after acupuncture. Cluster analysis of monocytes using Forward/Side scatter characteristics. The proportion of monocyte increased from 4.8%–11.8% in PBMC. The granulocyte decreased from 74.4%–53.7%. (D,E) The J774 Cell line was treated by ATP from the concentration of 2.53 nM–2.53 μM. After incubated for 24 h, the supernatant was detected by BDNF ELISA kit at 1, 2, 4, and 24 h. The result showed that ATP can induce the expression of BDNF with a dose-dependent, and reach a peak level at 4 h, and then decreased at 24 h.
Ijms 15 03234f2 1024
Table 1. Publications of acupuncture therapy published on PUBMED in the last 10 years.
Table 1. Publications of acupuncture therapy published on PUBMED in the last 10 years.
Animal research

ReferenceExperimentalTissueStimulationTime of treatmentAcupointSignal pathwayResult
(Kim, et al., 2013) [56]cerebral ischemia in micecerebral cortexElectroacupuncture 2 HZ/1 mA/20 minEA preconditioning for 3 daysGV20/GV14Expression of BDNF increased (after 12 h)
(Zhao, et al., 2013) [52]cerebral ischemia-reperfusion injury(mouse)hippocampusElectroacupunctureonce daily for 7 daysBL17/GV20/BL23BDNF mRNA expressions Up-regulated
(Chen, et al., 2012) [55]cerebral ischemia/reperfusion (I/R) injury(rat)BloodElectroacupuncture/disperse wave of 1 and 20 Hz30 min treated/2 or 24 h after ischemia/reperfusionLI11/ST36PI3K/Akt signaling pathwayincrease BDNF and GDNF secretion levels in serum
(Kim, et al., 2012) [54]cerebral ischaemia(rat)ischaemic hemisphereElectroacupunctureonce daily for 2 weeksGV20BDNF/trkBincreased expression of BDNF/trkB protein
(Lee, et al., 2012) [61]spatial cognitive impairment induced by repeated corticosterone (CORT)(rat)hippocampusManual acupunctureonce daily for 21 daysTE5/HT7Up-regulate BDNF mRNA expressions levels
(Liang, et al., 2012) [50]chronic stress-induced depression (rat)prefrontal cortex and hippocampusElectroacupunctureonce every other day for 28 daysGV 20/EX-HN 3/PC 6Up-regulate BDNF mRNA and protein expression levels
(Park, et al., 2012) [67]depression-like behavioral changes (rat)prefrontal cortex (PFC)Manual acupuncture7 consecutive daysHT7/ST36increased expression of BDNF protein
(Wang, et al., 2012) [68]neck-incision pain ratsin the cervico-spinal cord (C1–C4)Electroacupuncture (1–2 mA, 2 Hz/100 Hz)30 minLI 18/PC 6-LI 4/ST 36-GB 34BDNF/trkB/trkADown-regulated for the BDNF mRNA, TrkA mRNA and TrkB mRNA
(Zhang, et al., 2012) [69]spinal cord transaction between T9 and T10 (mouse)cortex areaElectroacupunctureonce daily for 14 days“Governor Vessel” acupointsincreased expression of BDNF protein
(Hwang, et al., 2010) [11]Normal Wistar rats (13-week-old)in the dentate gyrus of hippocampusElectroacupunctureonce daily for 3 weeksST36/GV20BDNF/CREBincreased expression of BDNF protein
(Sun, et al., 2010) [70]glaucoma model in rabbitsretinaManual acupuncturetwice a day for 4 weeksEX-HN 7/GB 20/LR 2BDNF/Bcl-xlincreased expression of BDNF protein
(Hua, et al., 2009) [71]ovariectomized rat fracture modelfractural callus and blood samplesManual acupunctureonce daily for 4 weeksGB 30/ST 36/GB 34/BL 40BDNF/trkBincreased expression of BDNF/trkB protein
(Kim, et al., 2009) [10]middle cerebral artery occlusion (MCAO) ratsCerebral ischemia areaElectroacupuncture (30 min, 2/15 Hz)once daily for 16 daysGV20/GV14/LI11/ST36BDNF/trkBno significant change in BDNF
(Manni, et al., 2009) [72]cognition induced by social isolation in the mousehypothalamus, striatum and hippocampusElectroacupuncture (30 min, 1–4 Hz)once daily for 4 daysST36Decreased expression of BDNF protein
(Wang, et al., 2009)[73]spinal cord injury (rat)spinal cordElectroacupuncture (20 min, 1 mA, 2 Hz/15 Hz)once daily for 10 daysCV 4/ST 28increased expression of BDNF/trkB protein
(Jeon, et al., 2008) [74]MPTP induced Parkinson’s disease mouse modelsubstantia nigramanual acupunctureonce daily for 7 daysB34/SI3/BL62/ST36BDNF/CypAincreased expression of CypA following BDNF
(Chen, et al., 2007) [75]cats subjected to removal of adjacent gangliaL6 dorsal root ganglion (DRG)Electroacupuncture (30 min, 98 Hz)once daily for 7 daysST36/GB39/ST32/SP6Up-regulate BDNF mRNA and protein expression levels
(Liang, et al., 2002) [76]Parkinson’s disease rats model induced by transection of the medial forebrain bundle (MFB)ventral midbrain/ventral tegmental area/substantia nigraElectroacupuncture (30 min, 1–2 mA, 2/100 Hz)once daily for 24 daysGV 14/GV 21Up-regulate BDNF mRNA expressions levels
(Yun, et al., 2002) [48]stress-induced hippocampal degeneration ratshippocampusElectroacupuncture (30 min, 2 Hz)30 min (one time)ST36Up-regulate BDNF mRNA expressions levels

For clinical research

(Xia, et al., 2012) [77]Parkinson’s disease combined with depression patientsserumelectroacupuncture3 monthsGV 20/EX-HN 3/EX-HN 1/LR 3/SP 6increased expression of BDNF(compared with that before treatment)
(Moldenhauer, et al., 2010) [78]Spinal Cord InjuriesserumManual acupuncture<1 hwhole-body acupunctureDecreased expression of BDNF in 48 h after acupuncture

(The detail location of each acupoint can be refer to the website:

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top