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Int. J. Mol. Sci. 2014, 15(2), 2971-2990; doi:10.3390/ijms15022971
Article

A Genotoxic Stress-Responsive miRNA, miR-574-3p, Delays Cell Growth by Suppressing the Enhancer of Rudimentary Homolog Gene in Vitro

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Received: 15 October 2013; Accepted: 13 February 2014 / Published: 20 February 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: MicroRNA (miRNA) is a type of non-coding RNA that regulates the expression of its target genes by interacting with the complementary sequence of the target mRNA molecules. Recent evidence has shown that genotoxic stress induces miRNA expression, but the target genes involved and role in cellular responses remain unclear. We examined the role of miRNA in the cellular response to X-ray irradiation by studying the expression profiles of radio-responsive miRNAs and their target genes in cultured human cell lines. We found that expression of miR-574-3p was induced in the lung cancer cell line A549 by X-ray irradiation. Overexpression of miR-574-3p caused delayed growth in A549 cells. A predicted target site was detected in the 3'-untranslated region of the enhancer of the rudimentary homolog (ERH) gene, and transfected cells showed an interaction between the luciferase reporter containing the target sequences and miR-574-3p. Overexpression of miR-574-3p suppressed ERH protein production and delayed cell growth. This delay was confirmed by knockdown of ERH expression. Our study suggests that miR-574-3p may contribute to the regulation of the cell cycle in response to X-ray irradiation via suppression of ERH protein production.
Keywords: irradiation; cell growth delay; miR-574-3p; enhancer rudimentary homolog gene irradiation; cell growth delay; miR-574-3p; enhancer rudimentary homolog gene
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ishikawa, K.-I.; Ishikawa, A.; Shoji, Y.; Imai, T. A Genotoxic Stress-Responsive miRNA, miR-574-3p, Delays Cell Growth by Suppressing the Enhancer of Rudimentary Homolog Gene in Vitro. Int. J. Mol. Sci. 2014, 15, 2971-2990.

AMA Style

Ishikawa K-I, Ishikawa A, Shoji Y, Imai T. A Genotoxic Stress-Responsive miRNA, miR-574-3p, Delays Cell Growth by Suppressing the Enhancer of Rudimentary Homolog Gene in Vitro. International Journal of Molecular Sciences. 2014; 15(2):2971-2990.

Chicago/Turabian Style

Ishikawa, Ken-ichi; Ishikawa, Atsuko; Shoji, Yoshimi; Imai, Takashi. 2014. "A Genotoxic Stress-Responsive miRNA, miR-574-3p, Delays Cell Growth by Suppressing the Enhancer of Rudimentary Homolog Gene in Vitro." Int. J. Mol. Sci. 15, no. 2: 2971-2990.



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