The Proteasome Activator PA28γ, a Negative Regulator of p53, Is Transcriptionally Up-Regulated by p53
Abstract
:1. Introduction
2. Results
2.1. Determination of the Transcription Start Site (TSS) of the Human PA28γ Gene
2.2. Identification of the Basal Promoter of the Human PA28γ Gene
2.3. p53 Binds to the Promoter of the Human PA28γ Gene
2.4. p53 Stimulates the Transcription of Human PA28γ
2.5. p53-Induced Transcription of PA28γ Is Suppressed by PA28γ Itself
3. Discussion
4. Materials and Methods
4.1. Plasmid Constructs
4.2. Computational Analysis of the Promoter
4.3. Cell Culture and Transfection
4.4. Luciferase Assay
4.5. RLM-RACE Assay
4.6. ChIP Assay
4.7. Biotinylated DNA Affinity Precipitation
4.8. RNA Interference
4.9. Statistical Analysis
5. Conclusions
Acknowledgments
Conflicts of Interest
- Author ContributionsJ.-L.Z. conceived and designed the experiments. Z.-X.W., D.-M.Y., Y.-M.Z., X.Y., J.Z. and Z.-X.X. performed the experiments. Z.-X.W., D.-M.Y. and J.-L.Z. analyzed the data. J.-L.Z. wrote the manuscript. All authors read and approved the final manuscript.
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Wan, Z.-X.; Yuan, D.-M.; Zhuo, Y.-M.; Yi, X.; Zhou, J.; Xu, Z.-X.; Zhou, J.-L. The Proteasome Activator PA28γ, a Negative Regulator of p53, Is Transcriptionally Up-Regulated by p53. Int. J. Mol. Sci. 2014, 15, 2573-2584. https://doi.org/10.3390/ijms15022573
Wan Z-X, Yuan D-M, Zhuo Y-M, Yi X, Zhou J, Xu Z-X, Zhou J-L. The Proteasome Activator PA28γ, a Negative Regulator of p53, Is Transcriptionally Up-Regulated by p53. International Journal of Molecular Sciences. 2014; 15(2):2573-2584. https://doi.org/10.3390/ijms15022573
Chicago/Turabian StyleWan, Zhen-Xing, Dong-Mei Yuan, Yi-Ming Zhuo, Xin Yi, Ji Zhou, Zao-Xu Xu, and Jian-Lin Zhou. 2014. "The Proteasome Activator PA28γ, a Negative Regulator of p53, Is Transcriptionally Up-Regulated by p53" International Journal of Molecular Sciences 15, no. 2: 2573-2584. https://doi.org/10.3390/ijms15022573