Abstract: PA28γ (also called REGγ, 11Sγ or PSME3) negatively regulates p53 activity by promoting its nuclear export and/or degradation. Here, using the RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) method, we identified the transcription start site of the PA28γ gene. Assessment with the luciferase assay demonstrated that the sequence −193 to +16 is the basal promoter. Three p53 binding sites were found within the PA28γ promoter utilizing a bioinformatics approach and were confirmed by chromatin immunoprecipitation and biotinylated DNA affinity precipitation experiments. The p53 protein promotes PA28γ transcription, and p53-stimulated transcription of PA28γ can be inhibited by PA28γ itself. Our results suggest that PA28γ and p53 form a negative feedback loop, which maintains the balance of p53 and PA28γ in cells.
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Wan, Z.-X.; Yuan, D.-M.; Zhuo, Y.-M.; Yi, X.; Zhou, J.; Xu, Z.-X.; Zhou, J.-L. The Proteasome Activator PA28γ, a Negative Regulator of p53, Is Transcriptionally Up-Regulated by p53. Int. J. Mol. Sci. 2014, 15, 2573-2584.
Wan Z-X, Yuan D-M, Zhuo Y-M, Yi X, Zhou J, Xu Z-X, Zhou J-L. The Proteasome Activator PA28γ, a Negative Regulator of p53, Is Transcriptionally Up-Regulated by p53. International Journal of Molecular Sciences. 2014; 15(2):2573-2584.
Wan, Zhen-Xing; Yuan, Dong-Mei; Zhuo, Yi-Ming; Yi, Xin; Zhou, Ji; Xu, Zao-Xu; Zhou, Jian-Lin. 2014. "The Proteasome Activator PA28γ, a Negative Regulator of p53, Is Transcriptionally Up-Regulated by p53." Int. J. Mol. Sci. 15, no. 2: 2573-2584.