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Int. J. Mol. Sci. 2014, 15(2), 2573-2584; doi:10.3390/ijms15022573
Article

The Proteasome Activator PA28γ, a Negative Regulator of p53, Is Transcriptionally Up-Regulated by p53

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Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, College of Life Science, Hunan Normal University, Changsha 410081, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 23 January 2014 / Revised: 8 February 2014 / Accepted: 8 February 2014 / Published: 13 February 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

PA28γ (also called REGγ, 11Sγ or PSME3) negatively regulates p53 activity by promoting its nuclear export and/or degradation. Here, using the RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) method, we identified the transcription start site of the PA28γ gene. Assessment with the luciferase assay demonstrated that the sequence −193 to +16 is the basal promoter. Three p53 binding sites were found within the PA28γ promoter utilizing a bioinformatics approach and were confirmed by chromatin immunoprecipitation and biotinylated DNA affinity precipitation experiments. The p53 protein promotes PA28γ transcription, and p53-stimulated transcription of PA28γ can be inhibited by PA28γ itself. Our results suggest that PA28γ and p53 form a negative feedback loop, which maintains the balance of p53 and PA28γ in cells.
Keywords: PA28γ; p53; promoter; transcription regulation; PSME3; negative feedback regulation PA28γ; p53; promoter; transcription regulation; PSME3; negative feedback regulation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Wan, Z.-X.; Yuan, D.-M.; Zhuo, Y.-M.; Yi, X.; Zhou, J.; Xu, Z.-X.; Zhou, J.-L. The Proteasome Activator PA28γ, a Negative Regulator of p53, Is Transcriptionally Up-Regulated by p53. Int. J. Mol. Sci. 2014, 15, 2573-2584.

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