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Int. J. Mol. Sci. 2014, 15(2), 2255-2273; doi:10.3390/ijms15022255
Article

Isolation and Molecular Characterization of Biofouling Bacteria and Profiling of Quorum Sensing Signal Molecules from Membrane Bioreactor Activated Sludge

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Received: 16 December 2013; in revised form: 17 January 2014 / Accepted: 23 January 2014 / Published: 4 February 2014
(This article belongs to the Special Issue New Non (Limited)-Toxic Antifouling Solutions)
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Abstract: The formation of biofilm in a membrane bioreactor depends on the production of various signaling molecules like N-acyl homoserine lactones (AHLs). In the present study, a total of 200 bacterial strains were isolated from membrane bioreactor activated sludge and screened for AHLs production using two biosensor systems, Chromobacterium violaceum CV026 and Agrobacterium tumefaciens A136. A correlation between AHLs production and biofilm formation has been made among screened AHLs producing strains. The 16S rRNA gene sequence analysis revealed the dominance of Aeromonas and Enterobacter sp. in AHLs production; however few a species of Serratia, Leclercia, Pseudomonas, Klebsiella, Raoultella and Citrobacter were also identified. The chromatographic characterization of sludge extract showed the presence of a broad range of quorum sensing signal molecules. Further identification of sludge AHLs by thin layer chromatography bioassay and high performance liquid chromatography confirms the presence of C4-HSL, C6-HSL, C8-HSL, 3-oxo-C8-HSL, C10-HSL, C12-HSL, 3-oxo-C12-HSL and C14-HSL. The occurrence of AHLs in sludge extract and dominance of Aeromonas and Enterobacter sp. in activated sludge suggests the key role of these bacterial strains in AHLs production and thereby membrane fouling.
Keywords: membrane bioreactor; activated sludge; isolation of bacteria; violacein assay; β-galactosidase assay; quorum sensing signals; biofilm formation membrane bioreactor; activated sludge; isolation of bacteria; violacein assay; β-galactosidase assay; quorum sensing signals; biofilm formation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lade, H.; Paul, D.; Kweon, J.H. Isolation and Molecular Characterization of Biofouling Bacteria and Profiling of Quorum Sensing Signal Molecules from Membrane Bioreactor Activated Sludge. Int. J. Mol. Sci. 2014, 15, 2255-2273.

AMA Style

Lade H, Paul D, Kweon JH. Isolation and Molecular Characterization of Biofouling Bacteria and Profiling of Quorum Sensing Signal Molecules from Membrane Bioreactor Activated Sludge. International Journal of Molecular Sciences. 2014; 15(2):2255-2273.

Chicago/Turabian Style

Lade, Harshad; Paul, Diby; Kweon, Ji H. 2014. "Isolation and Molecular Characterization of Biofouling Bacteria and Profiling of Quorum Sensing Signal Molecules from Membrane Bioreactor Activated Sludge." Int. J. Mol. Sci. 15, no. 2: 2255-2273.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert