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Int. J. Mol. Sci. 2014, 15(2), 2003-2014; doi:10.3390/ijms15022003
Article

Amino Acid Composition, Molecular Weight Distribution and Gel Electrophoresis of Walnut (Juglans regia L.) Proteins and Protein Fractionations

1
, 2
 and 1,*
Received: 19 November 2013; in revised form: 19 January 2014 / Accepted: 22 January 2014 / Published: 27 January 2014
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract: As a by-product of oil production, walnut proteins are considered as an additional source of plant protein for human food. To make full use of the protein resource, a comprehensive understanding of composition and characteristics of walnut proteins are required. Walnut proteins have been fractionated and characterized in this study. Amino acid composition, molecular weight distribution and gel electrophoresis of walnut proteins and protein fractionations were analyzed. The proteins were sequentially separated into four fractions according to their solubility. Glutelin was the main component of the protein extract. The content of glutelin, albumin, globulin and prolamin was about 72.06%, 7.54%, 15.67% and 4.73% respectively. Glutelin, albumin and globulin have a balanced content of essential amino acids, except for methionine, with respect to the FAO pattern recommended for adults. SDS-PAGE patterns of albumin, globulin and glutelin showed several polypeptides with molecular weights 14.4 to 66.2 kDa. The pattern of walnut proteins in two-dimension electrophoresis (2-DE) showed that the isoelectric point was mainly in the range of 4.8–6.8. The results of size exclusion chromatogram indicated molecular weight of the major components of walnut proteins were between 3.54 and 81.76 kDa.
Keywords: walnut proteins; fractionation; amino acid composition; molecular weight distribution; 2-DE; HPLC walnut proteins; fractionation; amino acid composition; molecular weight distribution; 2-DE; HPLC
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Mao, X.; Hua, Y.; Chen, G. Amino Acid Composition, Molecular Weight Distribution and Gel Electrophoresis of Walnut (Juglans regia L.) Proteins and Protein Fractionations. Int. J. Mol. Sci. 2014, 15, 2003-2014.

AMA Style

Mao X, Hua Y, Chen G. Amino Acid Composition, Molecular Weight Distribution and Gel Electrophoresis of Walnut (Juglans regia L.) Proteins and Protein Fractionations. International Journal of Molecular Sciences. 2014; 15(2):2003-2014.

Chicago/Turabian Style

Mao, Xiaoying; Hua, Yufei; Chen, Guogang. 2014. "Amino Acid Composition, Molecular Weight Distribution and Gel Electrophoresis of Walnut (Juglans regia L.) Proteins and Protein Fractionations." Int. J. Mol. Sci. 15, no. 2: 2003-2014.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert