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Int. J. Mol. Sci. 2014, 15(2), 1804-1811; doi:10.3390/ijms15021804
Article

Protein A Detection Based on Quantum Dots-Antibody Bioprobe Using Fluorescence Coupled Capillary Electrophoresis

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Received: 11 December 2013; in revised form: 9 January 2014 / Accepted: 15 January 2014 / Published: 24 January 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: In this report, fluorescence detection coupled capillary electrophoresis (CE-FL) was used to detect Protein A. Antibody was first labeled with Cy5 and then mixed with quantum dots (QDs) to form QDs-antibody bioprobe. Further, we observed fluorescence resonance energy transfer (FRET) from QDs donor to Cy5 acceptor. The bioprobe was formed and brought QDs and Cy5 close enough to allow FRET to occur. After adding protein A, the FRET system was broken and caused the FRET signal to decrease. Thus, a new method for the determination of protein A was proposed based on the FRET signal changes. This study provides a new trail of thought for the detection of protein.
Keywords: QDs; FRET; bioprobe; capillary electrophoresis; protein A QDs; FRET; bioprobe; capillary electrophoresis; protein A
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Qiu, L.; Bi, Y.; Wang, C.; Li, J.; Guo, P.; Li, J.; He, W.; Wang, J.; Jiang, P. Protein A Detection Based on Quantum Dots-Antibody Bioprobe Using Fluorescence Coupled Capillary Electrophoresis. Int. J. Mol. Sci. 2014, 15, 1804-1811.

AMA Style

Qiu L, Bi Y, Wang C, Li J, Guo P, Li J, He W, Wang J, Jiang P. Protein A Detection Based on Quantum Dots-Antibody Bioprobe Using Fluorescence Coupled Capillary Electrophoresis. International Journal of Molecular Sciences. 2014; 15(2):1804-1811.

Chicago/Turabian Style

Qiu, Lin; Bi, Yanhua; Wang, Cheli; Li, Jingyan; Guo, Peilin; Li, Jinchen; He, Weijiang; Wang, Jianhao; Jiang, Pengju. 2014. "Protein A Detection Based on Quantum Dots-Antibody Bioprobe Using Fluorescence Coupled Capillary Electrophoresis." Int. J. Mol. Sci. 15, no. 2: 1804-1811.



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