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Int. J. Mol. Sci. 2014, 15(2), 1804-1811; doi:10.3390/ijms15021804

Protein A Detection Based on Quantum Dots-Antibody Bioprobe Using Fluorescence Coupled Capillary Electrophoresis

1
State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China
2
School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China
3
Shandong Academy of Medical Sciences, Jinan 250062, China
*
Authors to whom correspondence should be addressed.
Received: 11 December 2013 / Revised: 9 January 2014 / Accepted: 15 January 2014 / Published: 24 January 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

In this report, fluorescence detection coupled capillary electrophoresis (CE-FL) was used to detect Protein A. Antibody was first labeled with Cy5 and then mixed with quantum dots (QDs) to form QDs-antibody bioprobe. Further, we observed fluorescence resonance energy transfer (FRET) from QDs donor to Cy5 acceptor. The bioprobe was formed and brought QDs and Cy5 close enough to allow FRET to occur. After adding protein A, the FRET system was broken and caused the FRET signal to decrease. Thus, a new method for the determination of protein A was proposed based on the FRET signal changes. This study provides a new trail of thought for the detection of protein. View Full-Text
Keywords: QDs; FRET; bioprobe; capillary electrophoresis; protein A QDs; FRET; bioprobe; capillary electrophoresis; protein A
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Qiu, L.; Bi, Y.; Wang, C.; Li, J.; Guo, P.; Li, J.; He, W.; Wang, J.; Jiang, P. Protein A Detection Based on Quantum Dots-Antibody Bioprobe Using Fluorescence Coupled Capillary Electrophoresis. Int. J. Mol. Sci. 2014, 15, 1804-1811.

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