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Int. J. Mol. Sci. 2014, 15(11), 20101-20116; doi:10.3390/ijms151120101

Overexpression of the CaTIP1-1 Pepper Gene in Tobacco Enhances Resistance to Osmotic Stresses

1
College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China
2
Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China
3
College of Food and Biological Engineering, Xuchang University, Xuchang, Henan 461000, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 19 September 2014 / Revised: 27 October 2014 / Accepted: 29 October 2014 / Published: 4 November 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Both the gene expression and activity of water channel protein can control transmembrane water movement. We have reported the overexpression of CaTIP1-1, which caused a decrease in chilling tolerance in transgenic plants by increasing the size of the stomatal pore. CaTIP1-1 expression was strongly induced by salt and mannitol stresses in pepper (Capsicum annuum). However, its biochemical and physiological functions are still unknown in transgenic tobacco. In this study, transient expression of CaTIP1-1-GFP in tobacco suspension cells revealed that the protein was localized in the tonoplast. CaTIP1-1 overexpressed in radicle exhibited vigorous growth under high salt and mannitol treatments more than wild-type plants. The overexpression of CaTIP1-1 pepper gene in tobacco enhanced the antioxidant enzyme activities and increased transcription levels of reactive oxygen species-related gene expression under osmotic stresses. Moreover, the viability of transgenic tobacco cells was higher than the wild-type after exposure to stress. The pepper plants with silenced CaTIP1-1 in P70 decreased tolerance to salt and osmotic stresses using the detached leaf method. We concluded that the CaTIP1-1 gene plays an important role in response to osmotic stresses in tobacco. View Full-Text
Keywords: osmotic stress; antioxidant enzymes; Capsicum annuum L.; CaTIP1-1; tobacco osmotic stress; antioxidant enzymes; Capsicum annuum L.; CaTIP1-1; tobacco
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Yin, Y.-X.; Wang, S.-B.; Xiao, H.-J.; Zhang, H.-X.; Zhang, Z.; Jing, H.; Zhang, Y.-L.; Chen, R.-G.; Gong, Z.-H. Overexpression of the CaTIP1-1 Pepper Gene in Tobacco Enhances Resistance to Osmotic Stresses. Int. J. Mol. Sci. 2014, 15, 20101-20116.

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