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Int. J. Mol. Sci. 2014, 15(1), 1271-1283; doi:10.3390/ijms15011271
Article

Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides

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Received: 6 November 2013; in revised form: 19 December 2013 / Accepted: 3 January 2014 / Published: 17 January 2014
(This article belongs to the Section Molecular Recognition)
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Abstract: The binding of monosaccharides and short peptides to lymphocyte receptors (human CD69 and rat NKR-P1A) was first reported in 1994 and then in a number of subsequent publications. Based on this observation, numerous potentially high-affinity saccharide ligands have been synthesized over the last two decades in order to utilize their potential in antitumor therapy. Due to significant inconsistencies in their reported binding properties, we decided to re-examine the interaction between multiple ligands and CD69 or NKR-P1A. Using NMR titration and isothermal titration calorimetry we were unable to detect the binding of the tested ligands such as N-acetyl-d-hexosamines and oligopeptides to both receptors, which contradicts the previous observations published in more than twenty papers over the last fifteen years.
Keywords: CD69; NKR-P1A; NMR titration; calorimetry CD69; NKR-P1A; NMR titration; calorimetry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Rozbeský, D.; Krejzová, J.; Křenek, K.; Prchal, J.; Hrabal, R.; Košek, M.; Weignerová, L.; Fiore, M.; Dumy, P.; Renaudet, O.; Křen, V. Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides. Int. J. Mol. Sci. 2014, 15, 1271-1283.

AMA Style

Rozbeský D, Krejzová J, Křenek K, Prchal J, Hrabal R, Košek M, Weignerová L, Fiore M, Dumy P, Renaudet O, Křen V. Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides. International Journal of Molecular Sciences. 2014; 15(1):1271-1283.

Chicago/Turabian Style

Rozbeský, Daniel; Krejzová, Jana; Křenek, Karel; Prchal, Jan; Hrabal, Richard; Košek, Milan; Weignerová, Lenka; Fiore, Michele; Dumy, Pascal; Renaudet, Olivier; Křen, Vladimír. 2014. "Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides." Int. J. Mol. Sci. 15, no. 1: 1271-1283.


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