Probing Antigen-Antibody Interaction Using Fluorescence Coupled Capillary Electrophoresis

doi:10.3390/ijms140919146

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Int. J. Mol. Sci. 2013, 14(9), 19146-19154; doi:10.3390/ijms140919146

Article

Probing Antigen-Antibody Interaction Using Fluorescence Coupled Capillary Electrophoresis

Jianhao Wang^1,†

, Lin Qiu^1,†

, Cheli Wang¹

, Yue Zhang¹

, Jingyan Li¹

, Jiang Xia^2,* and Pengju Jiang^1,*

+ Authors' affiliations

Received: 23 August 2013 / Revised: 4 September 2013 / Accepted: 5 September 2013 / Published: 17 September 2013

(This article belongs to the Special Issue Molecular Bases of Cancer Research)

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Abstract: In this report, the use of fluorescence detection coupled capillary electrophoresis (CE-FL) allowed us to fully characterize the antigen-antibody interaction. CE-FL allowed separation of unbound quantum dots (QDs) and ligand bound QDs and also revealed an ordered assembly of biomolecules on QDs. Further, we observed FRET from QDs donor to DyLight acceptor, which were covalently conjugated with human IgG and goat anti-human IgG, respectively. The immunocomplex was formed and the mutual affinity of the antigen and antibody brought QDs and DyLight close enough to allow FRET to occur. This novel CE-based technique can be easily extended to other FRET systems based on QDs and may have potential application in the detection of antibodies.

Keywords: QDs; FRET; immunocomplex; capillary electrophoresis

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, J.; Qiu, L.; Wang, C.; Zhang, Y.; Li, J.; Xia, J.; Jiang, P. Probing Antigen-Antibody Interaction Using Fluorescence Coupled Capillary Electrophoresis. Int. J. Mol. Sci. 2013, 14, 19146-19154.

AMA Style

Wang J, Qiu L, Wang C, Zhang Y, Li J, Xia J, Jiang P. Probing Antigen-Antibody Interaction Using Fluorescence Coupled Capillary Electrophoresis. International Journal of Molecular Sciences. 2013; 14(9):19146-19154.

Chicago/Turabian Style

Wang, Jianhao; Qiu, Lin; Wang, Cheli; Zhang, Yue; Li, Jingyan; Xia, Jiang; Jiang, Pengju. 2013. "Probing Antigen-Antibody Interaction Using Fluorescence Coupled Capillary Electrophoresis." Int. J. Mol. Sci. 14, no. 9: 19146-19154.

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