Article Versions
Related Info
More by Authors
Share This Article
  •  CiteULike
  •  Facebook
  •  Mendeley
  •  Twitter
  • More services
Int. J. Mol. Sci. 2013, 14(9), 19146-19154; doi:10.3390/ijms140919146
Article

Probing Antigen-Antibody Interaction Using Fluorescence Coupled Capillary Electrophoresis

1,†email, 1,†email, 1email, 1email, 1email, 2,* email and 1,* email
Received: 23 August 2013; in revised form: 4 September 2013 / Accepted: 5 September 2013 / Published: 17 September 2013
(This article belongs to the Special Issue Molecular Bases of Cancer Research)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In this report, the use of fluorescence detection coupled capillary electrophoresis (CE-FL) allowed us to fully characterize the antigen-antibody interaction. CE-FL allowed separation of unbound quantum dots (QDs) and ligand bound QDs and also revealed an ordered assembly of biomolecules on QDs. Further, we observed FRET from QDs donor to DyLight acceptor, which were covalently conjugated with human IgG and goat anti-human IgG, respectively. The immunocomplex was formed and the mutual affinity of the antigen and antibody brought QDs and DyLight close enough to allow FRET to occur. This novel CE-based technique can be easily extended to other FRET systems based on QDs and may have potential application in the detection of antibodies.
Keywords: QDs; FRET; immunocomplex; capillary electrophoresis
PDF Full-text Download PDF Full-Text [369 KB, uploaded 19 June 2014 05:09 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Wang, J.; Qiu, L.; Wang, C.; Zhang, Y.; Li, J.; Xia, J.; Jiang, P. Probing Antigen-Antibody Interaction Using Fluorescence Coupled Capillary Electrophoresis. Int. J. Mol. Sci. 2013, 14, 19146-19154.

AMA Style

Wang J, Qiu L, Wang C, Zhang Y, Li J, Xia J, Jiang P. Probing Antigen-Antibody Interaction Using Fluorescence Coupled Capillary Electrophoresis. International Journal of Molecular Sciences. 2013; 14(9):19146-19154.

Chicago/Turabian Style

Wang, Jianhao; Qiu, Lin; Wang, Cheli; Zhang, Yue; Li, Jingyan; Xia, Jiang; Jiang, Pengju. 2013. "Probing Antigen-Antibody Interaction Using Fluorescence Coupled Capillary Electrophoresis." Int. J. Mol. Sci. 14, no. 9: 19146-19154.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert