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Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice
Departments of Anatomy, Weifang Medical University, Weifang 261053, China
Departments of Pathology, Weifang Medical University, Weifang 261053, China
Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 19 July 2013; in revised form: 29 July 2013 / Accepted: 9 August 2013 / Published: 29 August 2013
Abstract: The purpose of this study was to investigate the possible protective effect of N-acetylserotonin (NAS) against acute hepatic ischemia-reperfusion (I/R) injury in mice. Adult male mice were randomly divided into three groups: sham, I/R, and I/R + NAS. The hepatic I/R injury model was generated by clamping the hepatic artery, portal vein, and common bile duct with a microvascular bulldog clamp for 30 min, and then removing the clamp and allowing reperfusion for 6 h. Morphologic changes and hepatocyte apoptosis were evaluated by hematoxylin-eosin (HE) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Activated caspase-3 expression was evaluated by immunohistochemistry and Western blot. The activation of aspartate aminotransferase (AST), malondialdehyde (MDA), and superoxide dismutase (SOD) was evaluated by enzyme-linked immunosorbent assay (ELISA). The data show that NAS rescued hepatocyte morphological damage and dysfunction, decreased the number of apoptotic hepatocytes, and reduced caspase-3 activation. Our work demonstrates that NAS ameliorates hepatic IR injury.
Keywords: N-acetylserotonin; newborn mouse; hepatic ischemia-reperfusion injury; apoptosis
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MDPI and ACS Style
Yu, S.; Zheng, J.; Jiang, Z.; Shi, C.; Li, J.; Du, X.; Wang, H.; Jiang, J.; Wang, X. Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice. Int. J. Mol. Sci. 2013, 14, 17680-17693.
Yu S, Zheng J, Jiang Z, Shi C, Li J, Du X, Wang H, Jiang J, Wang X. Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice. International Journal of Molecular Sciences. 2013; 14(9):17680-17693.
Yu, Shuna; Zheng, Jie; Jiang, Zhengchen; Shi, Caixing; Li, Jin; Du, Xiaodong; Wang, Hailiang; Jiang, Jiying; Wang, Xin. 2013. "Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice." Int. J. Mol. Sci. 14, no. 9: 17680-17693.