Int. J. Mol. Sci. 2013, 14(9), 17680-17693; doi:10.3390/ijms140917680
Article

Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice

1,†email, 2,†email, 1email, 1email, 1email, 1email, 1email, 1,* email and 3,* email
Received: 19 July 2013; in revised form: 29 July 2013 / Accepted: 9 August 2013 / Published: 29 August 2013
(This article belongs to the Section Molecular Pathology)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The purpose of this study was to investigate the possible protective effect of N-acetylserotonin (NAS) against acute hepatic ischemia-reperfusion (I/R) injury in mice. Adult male mice were randomly divided into three groups: sham, I/R, and I/R + NAS. The hepatic I/R injury model was generated by clamping the hepatic artery, portal vein, and common bile duct with a microvascular bulldog clamp for 30 min, and then removing the clamp and allowing reperfusion for 6 h. Morphologic changes and hepatocyte apoptosis were evaluated by hematoxylin-eosin (HE) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Activated caspase-3 expression was evaluated by immunohistochemistry and Western blot. The activation of aspartate aminotransferase (AST), malondialdehyde (MDA), and superoxide dismutase (SOD) was evaluated by enzyme-linked immunosorbent assay (ELISA). The data show that NAS rescued hepatocyte morphological damage and dysfunction, decreased the number of apoptotic hepatocytes, and reduced caspase-3 activation. Our work demonstrates that NAS ameliorates hepatic IR injury.
Keywords: N-acetylserotonin; newborn mouse; hepatic ischemia-reperfusion injury; apoptosis
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MDPI and ACS Style

Yu, S.; Zheng, J.; Jiang, Z.; Shi, C.; Li, J.; Du, X.; Wang, H.; Jiang, J.; Wang, X. Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice. Int. J. Mol. Sci. 2013, 14, 17680-17693.

AMA Style

Yu S, Zheng J, Jiang Z, Shi C, Li J, Du X, Wang H, Jiang J, Wang X. Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice. International Journal of Molecular Sciences. 2013; 14(9):17680-17693.

Chicago/Turabian Style

Yu, Shuna; Zheng, Jie; Jiang, Zhengchen; Shi, Caixing; Li, Jin; Du, Xiaodong; Wang, Hailiang; Jiang, Jiying; Wang, Xin. 2013. "Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice." Int. J. Mol. Sci. 14, no. 9: 17680-17693.


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