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Int. J. Mol. Sci. 2013, 14(9), 17680-17693; doi:10.3390/ijms140917680
Article

Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice

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1,*  and 3,*
1 Departments of Anatomy, Weifang Medical University, Weifang 261053, China 2 Departments of Pathology, Weifang Medical University, Weifang 261053, China 3 Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 19 July 2013 / Revised: 29 July 2013 / Accepted: 9 August 2013 / Published: 29 August 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

The purpose of this study was to investigate the possible protective effect of N-acetylserotonin (NAS) against acute hepatic ischemia-reperfusion (I/R) injury in mice. Adult male mice were randomly divided into three groups: sham, I/R, and I/R + NAS. The hepatic I/R injury model was generated by clamping the hepatic artery, portal vein, and common bile duct with a microvascular bulldog clamp for 30 min, and then removing the clamp and allowing reperfusion for 6 h. Morphologic changes and hepatocyte apoptosis were evaluated by hematoxylin-eosin (HE) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Activated caspase-3 expression was evaluated by immunohistochemistry and Western blot. The activation of aspartate aminotransferase (AST), malondialdehyde (MDA), and superoxide dismutase (SOD) was evaluated by enzyme-linked immunosorbent assay (ELISA). The data show that NAS rescued hepatocyte morphological damage and dysfunction, decreased the number of apoptotic hepatocytes, and reduced caspase-3 activation. Our work demonstrates that NAS ameliorates hepatic IR injury.
Keywords: N-acetylserotonin; newborn mouse; hepatic ischemia-reperfusion injury; apoptosis N-acetylserotonin; newborn mouse; hepatic ischemia-reperfusion injury; apoptosis
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Yu, S.; Zheng, J.; Jiang, Z.; Shi, C.; Li, J.; Du, X.; Wang, H.; Jiang, J.; Wang, X. Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice. Int. J. Mol. Sci. 2013, 14, 17680-17693.

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