Abstract: Primitive proteins are proposed to have utilized organic cofactors more frequently than transition metals in redox reactions. Thus, an experimental validation on whether a protein constituted solely by early amino acids and an organic cofactor can perform electron transfer activity is an urgent challenge. In this paper, by substituting “late amino acids (C, F, M, T, W, and Y)” with “early amino acids (A, L, and V)” in a flavodoxin, we constructed a flavodoxin mutant and evaluated its characteristic properties. The major results showed that: (1) The flavodoxin mutant has structural characteristics similar to wild-type protein; (2) Although the semiquinone and hydroquinone flavodoxin mutants possess lower stability than the corresponding form of wild-type flavodoxin, the redox potential of double electron reduction Em,7 (fld) reached −360 mV, indicating that the flavodoxin mutant constituted solely by early amino acids can exert effective electron transfer activity.
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Lu, M.-F.; Ji, H.-F.; Li, T.-X.; Kang, S.-K.; Zhang, Y.-J.; Zheng, J.-F.; Tian, T.; Jia, X.-S.; Lin, X.-M.; Zhang, H.-Y. Reconstructing a Flavodoxin Oxidoreductase with Early Amino Acids. Int. J. Mol. Sci. 2013, 14, 12843-12852.
Lu M-F, Ji H-F, Li T-X, Kang S-K, Zhang Y-J, Zheng J-F, Tian T, Jia X-S, Lin X-M, Zhang H-Y. Reconstructing a Flavodoxin Oxidoreductase with Early Amino Acids. International Journal of Molecular Sciences. 2013; 14(6):12843-12852.
Lu, Ming-Feng; Ji, Hong-Fang; Li, Ting-Xuan; Kang, Shou-Kai; Zhang, Yue-Jie; Zheng, Jue-Fei; Tian, Tian; Jia, Xi-Shuai; Lin, Xing-Ming; Zhang, Hong-Yu. 2013. "Reconstructing a Flavodoxin Oxidoreductase with Early Amino Acids." Int. J. Mol. Sci. 14, no. 6: 12843-12852.