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Reconstructing a Flavodoxin Oxidoreductase with Early Amino Acids
School of Life Sciences, Shandong Normal University, Jinan 250014, China
School of Life Sciences, Shandong University of Technology, Zibo 255091, China
National Key Laboratory of Crop Genetic Improvement, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Center for Bioinformatics, Huazhong Agricultural University, Wuhan 430070, China
* Author to whom correspondence should be addressed.
Received: 9 April 2013; in revised form: 24 May 2013 / Accepted: 13 June 2013 / Published: 19 June 2013
Abstract: Primitive proteins are proposed to have utilized organic cofactors more frequently than transition metals in redox reactions. Thus, an experimental validation on whether a protein constituted solely by early amino acids and an organic cofactor can perform electron transfer activity is an urgent challenge. In this paper, by substituting “late amino acids (C, F, M, T, W, and Y)” with “early amino acids (A, L, and V)” in a flavodoxin, we constructed a flavodoxin mutant and evaluated its characteristic properties. The major results showed that: (1) The flavodoxin mutant has structural characteristics similar to wild-type protein; (2) Although the semiquinone and hydroquinone flavodoxin mutants possess lower stability than the corresponding form of wild-type flavodoxin, the redox potential of double electron reduction Em,7 (fld) reached −360 mV, indicating that the flavodoxin mutant constituted solely by early amino acids can exert effective electron transfer activity.
Keywords: origin of life; primitive redox protein; cofactor; early amino acid
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Lu, M.-F.; Ji, H.-F.; Li, T.-X.; Kang, S.-K.; Zhang, Y.-J.; Zheng, J.-F.; Tian, T.; Jia, X.-S.; Lin, X.-M.; Zhang, H.-Y. Reconstructing a Flavodoxin Oxidoreductase with Early Amino Acids. Int. J. Mol. Sci. 2013, 14, 12843-12852.
Lu M-F, Ji H-F, Li T-X, Kang S-K, Zhang Y-J, Zheng J-F, Tian T, Jia X-S, Lin X-M, Zhang H-Y. Reconstructing a Flavodoxin Oxidoreductase with Early Amino Acids. International Journal of Molecular Sciences. 2013; 14(6):12843-12852.
Lu, Ming-Feng; Ji, Hong-Fang; Li, Ting-Xuan; Kang, Shou-Kai; Zhang, Yue-Jie; Zheng, Jue-Fei; Tian, Tian; Jia, Xi-Shuai; Lin, Xing-Ming; Zhang, Hong-Yu. 2013. "Reconstructing a Flavodoxin Oxidoreductase with Early Amino Acids." Int. J. Mol. Sci. 14, no. 6: 12843-12852.