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Prevention of Intra-Abdominal Adhesion by Bi-Layer Electrospun Membrane
Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiaotong University School of Medicine, 600 Yishan Road, Shanghai 200233, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 26 April 2013; in revised form: 27 May 2013 / Accepted: 29 May 2013 / Published: 4 June 2013
Abstract: The aim of this study was to compare the anti-adhesion efficacy of a bi-layer electrospun fibrous membrane consisting of hyaluronic acid-loaded poly(ε-caprolactone) (PCL) fibrous membrane as the inner layer and PCL fibrous membrane as the outer layer with a single-layer PCL electrospun fibrous membrane in a rat cecum abrasion model. The rat model utilized a cecal abrasion and abdominal wall insult surgical protocol. The bi-layer and PCL membranes were applied between the cecum and the abdominal wall, respectively. Control animals did not receive any treatment. After postoperative day 14, a visual semiquantitative grading scale was used to grade the extent of adhesion. Histological analysis was performed to reveal the features of adhesion tissues. Bi-layer membrane treated animals showed significantly lower adhesion scores than control animals (p < 0.05) and a lower adhesion score compared with the PCL membrane. Histological analysis of the bi-layer membrane treated rat rarely demonstrated tissue adhesion while that of the PCL membrane treated rat and control rat showed loose and dense adhesion tissues, respectively. Bi-layer membrane can efficiently prevent adhesion formation in abdominal cavity and showed a significantly decreased adhesion tissue formation compared with the control.
Keywords: postoperative adhesion; hyaluronic acid; electrospun; barrier; adhesion prevention
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Jiang, S.; Wang, W.; Yan, H.; Fan, C. Prevention of Intra-Abdominal Adhesion by Bi-Layer Electrospun Membrane. Int. J. Mol. Sci. 2013, 14, 11861-11870.
Jiang S, Wang W, Yan H, Fan C. Prevention of Intra-Abdominal Adhesion by Bi-Layer Electrospun Membrane. International Journal of Molecular Sciences. 2013; 14(6):11861-11870.
Jiang, Shichao; Wang, Wei; Yan, Hede; Fan, Cunyi. 2013. "Prevention of Intra-Abdominal Adhesion by Bi-Layer Electrospun Membrane." Int. J. Mol. Sci. 14, no. 6: 11861-11870.