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Characterization of the Pinus massoniana Transcriptional Response to Bursaphelenchus xylophilus Infection Using Suppression Subtractive Hybridization
College of Forestry, Beijing Forestry University, Beijing 100083, China
Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China
College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong, China
* Authors to whom correspondence should be addressed.
Received: 8 March 2013; in revised form: 3 May 2013 / Accepted: 14 May 2013 / Published: 28 May 2013
Abstract: Pine wilt disease (PWD) caused by pine wood nematode (PWN), Bursaphelenchus xylophilus, is the most destructive diseases of pine and poses a threat of serious economic losses worldwide. Although several of the mechanisms involved in disease progression have been discovered, the molecular response of Pinus massoniana to PWN infection has not been explored. We constructed four subtractive suppression hybridization cDNA libraries by taking time-course samples from PWN-inoculated Masson pine trees. One-hundred forty-four significantly differentially expressed sequence tags (ESTs) were identified, and 124 high-quality sequences with transcriptional features were selected for gene ontology (GO) and individual gene analyses. There were marked differences in the types of transcripts, as well as in the timing and levels of transcript expression in the pine trees following PWN inoculation. Genes involved in signal transduction, transcription and translation and secondary metabolism were highly expressed after 24 h and 72 h, while stress response genes were highly expressed only after 72 h. Certain transcripts responding to PWN infection were discriminative; pathogenesis and cell wall-related genes were more abundant, while detoxification or redox process-related genes were less abundant. This study provides new insights into the molecular mechanisms that control the biochemical and physiological responses of pine trees to PWN infection, particularly during the initial stage of infection.
Keywords: pine wilt disease; differentially expressed genes; suppression subtractive hybridization; Pinus massoniana
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MDPI and ACS Style
Xu, L.; Liu, Z.-Y.; Zhang, K.; Lu, Q.; Liang, J.; Zhang, X.-Y. Characterization of the Pinus massoniana Transcriptional Response to Bursaphelenchus xylophilus Infection Using Suppression Subtractive Hybridization. Int. J. Mol. Sci. 2013, 14, 11356-11375.
Xu L, Liu Z-Y, Zhang K, Lu Q, Liang J, Zhang X-Y. Characterization of the Pinus massoniana Transcriptional Response to Bursaphelenchus xylophilus Infection Using Suppression Subtractive Hybridization. International Journal of Molecular Sciences. 2013; 14(6):11356-11375.
Xu, Liang; Liu, Zhen-Yu; Zhang, Kai; Lu, Quan; Liang, Jun; Zhang, Xing-Yao. 2013. "Characterization of the Pinus massoniana Transcriptional Response to Bursaphelenchus xylophilus Infection Using Suppression Subtractive Hybridization." Int. J. Mol. Sci. 14, no. 6: 11356-11375.