Int. J. Mol. Sci. 2013, 14(6), 11157-11170; doi:10.3390/ijms140611157
Article

Isolation and Enhancement of a Homogenous in Vitro Human Hertwig’s Epithelial Root Sheath Cell Population

1,* email, 2,* email, 3email, 4email, 3email, 1email, 1email, 5email and 1,* email
Received: 9 April 2013; in revised form: 9 May 2013 / Accepted: 22 May 2013 / Published: 27 May 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Hertwig’s epithelial root sheath (HERS) cells play a pivotal role during root formation of the tooth and are able to form cementum-like tissue. The aim of the present study was to establish a HERS cell line for molecular and biochemical studies using a selective digestion method. Selective digestion was performed by the application of trypsin-EDTA for 2 min, which led to the detachment of fibroblast-like-cells, with the rounded cells attached to the culture plate. The HERS cells displayed a typical cuboidal/squamous-shaped appearance. Characterization of the HERS cells using immunofluorescence staining and flow cytometry analysis showed that these cells expressed pan-cytokeratin, E-cadherin, and p63 as epithelial markers. Moreover, RT-PCR confirmed that these cells expressed epithelial-related genes, such as cytokeratin 14, E-cadherin, and ΔNp63. Additionally, HERS cells showed low expression of CD44 and CD105 with absence of CD34 and amelogenin expressions. In conclusion, HERS cells have been successfully isolated using a selective digestion method, thus enabling future studies on the roles of these cells in the formation of cementum-like tissue in vitro.
Keywords: Hertwig’s epithelial root sheath (HERS); homogenous cell culture; selective digestion; characterization
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MDPI and ACS Style

Farea, M.; Halim, A.S.; Abdullah, N.A.; Lim, C.K.; Mokhtar, K.I.; Berahim, Z.; Mokhtar, K.; Rani, A.Q.; Husein, A. Isolation and Enhancement of a Homogenous in Vitro Human Hertwig’s Epithelial Root Sheath Cell Population. Int. J. Mol. Sci. 2013, 14, 11157-11170.

AMA Style

Farea M, Halim AS, Abdullah NA, Lim CK, Mokhtar KI, Berahim Z, Mokhtar K, Rani AQ, Husein A. Isolation and Enhancement of a Homogenous in Vitro Human Hertwig’s Epithelial Root Sheath Cell Population. International Journal of Molecular Sciences. 2013; 14(6):11157-11170.

Chicago/Turabian Style

Farea, Manal; Halim, Ahmad S.; Abdullah, Nurul A.; Lim, Chin K.; Mokhtar, Khairani I.; Berahim, Zurairah; Mokhtar, Kasmawati; Rani, Abdul Q.; Husein, Adam. 2013. "Isolation and Enhancement of a Homogenous in Vitro Human Hertwig’s Epithelial Root Sheath Cell Population." Int. J. Mol. Sci. 14, no. 6: 11157-11170.

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