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Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3'OH-T-2
Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, China
* Author to whom correspondence should be addressed.
Received: 10 April 2013; in revised form: 12 May 2013 / Accepted: 17 May 2013 / Published: 23 May 2013
Abstract: The transmission of T-2 toxin and its metabolites into the edible tissues of poultry has potential effects on human health. We report that T-2 toxin significantly induces CYP1A4 and CYP1A5 expression in chicken embryonic hepatocyte cells. The enzyme activity assays of CYP1A4 and CYP1A5 heterologously expressed in HeLa cells indicate that only CYP1A5 metabolizes T-2 to 3'OH-T-2 by the 3'-hydroxylation of isovaleryl groups. In vitro enzyme assays of recombinant CYP1A5 expressed in DH5α further confirm that CYP1A5 can convert T-2 into TC-1 (3'OH-T-2). Therefore, CYP1A5 is critical for the metabolism of trichothecene mycotoxin in chickens.
Keywords: T-2 toxin; chicken CYP1A5; metabolism
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Shang, S.; Jiang, J.; Deng, Y. Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3'OH-T-2. Int. J. Mol. Sci. 2013, 14, 10809-10818.
Shang S, Jiang J, Deng Y. Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3'OH-T-2. International Journal of Molecular Sciences. 2013; 14(6):10809-10818.
Shang, Shufeng; Jiang, Jun; Deng, Yiqun. 2013. "Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3'OH-T-2." Int. J. Mol. Sci. 14, no. 6: 10809-10818.