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Environmental Stresses Induce Misfolded Protein Aggregation in Plant Cells in a Microtubule-Dependent Manner
Laboratory of Fruit Genetic Engineering, the Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400-0005, Japan
* Author to whom correspondence should be addressed.
Received: 28 January 2013; in revised form: 25 March 2013 / Accepted: 27 March 2013 / Published: 10 April 2013
Abstract: Misfolded protein aggregation in mammalian cells is one of the cellular responses to environmental stresses. However, the aggregation of misfolded proteins in plant cells exposed to environmental stresses is still poorly understood. Here, we report the misfolded protein aggregation in plant cells in response to environmental stresses, including ultraviolet (UV) radiation, heat stress and cold stress. Treatment of grape and tobacco cultured cells with MG-132, a proteasome inhibitor, induced misfolded protein aggregation. All of the environmental stresses examined induced the endoplasmic reticulum (ER) stress response in the cells. The cells under ER stress showed aggregation of misfolded proteins. The misfolded protein aggregation was completely inhibited by treatment of the cells with trichostatin A or colchicine, suggesting that the misfolded proteins might be aggregated in plant cells in a microtubule-dependent manner. Detected aggregates were initially observed immediately after exposure to the environmental stresses (1 min after UV radiation, 5 min after heat stress exposure, and 15 min after cold stress exposure). Based on these findings, we hypothesize that environmental stresses induce misfolded protein aggregation in plant cells in a microtubule-dependent manner.
Keywords: environmental stress; ER stress; misfolded protein; protein aggregate
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Nakajima, Y.; Suzuki, S. Environmental Stresses Induce Misfolded Protein Aggregation in Plant Cells in a Microtubule-Dependent Manner. Int. J. Mol. Sci. 2013, 14, 7771-7783.
Nakajima Y, Suzuki S. Environmental Stresses Induce Misfolded Protein Aggregation in Plant Cells in a Microtubule-Dependent Manner. International Journal of Molecular Sciences. 2013; 14(4):7771-7783.
Nakajima, Yuko; Suzuki, Shunji. 2013. "Environmental Stresses Induce Misfolded Protein Aggregation in Plant Cells in a Microtubule-Dependent Manner." Int. J. Mol. Sci. 14, no. 4: 7771-7783.