Int. J. Mol. Sci. 2013, 14(4), 7457-7467; doi:10.3390/ijms14047457
Article

Characterization and Expression of the Gene Encoding En-MAPK1, an Intestinal Cell Kinase (ICK)-like Kinase Activated by the Autocrine Pheromone-Signaling Loop in the Polar Ciliate, Euplotes nobilii

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Received: 15 February 2013; in revised form: 21 March 2013 / Accepted: 21 March 2013 / Published: 3 April 2013
(This article belongs to the Special Issue Signalling Molecules and Signal Transduction in Cells)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In the protozoan ciliate Euplotes, a transduction pathway resulting in a mitogenic cell growth response is activated by autocrine receptor binding of cell type-specific, water-borne signaling protein pheromones. In Euplotes raikovi, a marine species of temperate waters, this transduction pathway was previously shown to involve the phosphorylation of a nuclear protein kinase structurally similar to the intestinal-cell and male germ cell-associated kinases described in mammals. In E. nobilii, which is phylogenetically closely related to E. raikovi but inhabits Antarctic and Arctic waters, we have now characterized a gene encoding a structurally homologous kinase. The expression of this gene requires +1 translational frameshifting and a process of intron splicing for the production of the active protein, designated En-MAPK1, which contains amino acid substitutions of potential significance for cold-adaptation.
Keywords: protein kinase; signal transduction; gene structure and expression; translational frameshifting; polar ciliates
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MDPI and ACS Style

Candelori, A.; Luporini, P.; Alimenti, C.; Vallesi, A. Characterization and Expression of the Gene Encoding En-MAPK1, an Intestinal Cell Kinase (ICK)-like Kinase Activated by the Autocrine Pheromone-Signaling Loop in the Polar Ciliate, Euplotes nobilii. Int. J. Mol. Sci. 2013, 14, 7457-7467.

AMA Style

Candelori A, Luporini P, Alimenti C, Vallesi A. Characterization and Expression of the Gene Encoding En-MAPK1, an Intestinal Cell Kinase (ICK)-like Kinase Activated by the Autocrine Pheromone-Signaling Loop in the Polar Ciliate, Euplotes nobilii. International Journal of Molecular Sciences. 2013; 14(4):7457-7467.

Chicago/Turabian Style

Candelori, Annalisa; Luporini, Pierangelo; Alimenti, Claudio; Vallesi, Adriana. 2013. "Characterization and Expression of the Gene Encoding En-MAPK1, an Intestinal Cell Kinase (ICK)-like Kinase Activated by the Autocrine Pheromone-Signaling Loop in the Polar Ciliate, Euplotes nobilii." Int. J. Mol. Sci. 14, no. 4: 7457-7467.

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