Int. J. Mol. Sci. 2013, 14(4), 7032-7047; doi:10.3390/ijms14047032
Article

Disruption of AtWNK8 Enhances Tolerance of Arabidopsis to Salt and Osmotic Stresses via Modulating Proline Content and Activities of Catalase and Peroxidase

1 State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Root Biology Center, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China 2 Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China 3 Life science and Technology School, Zhanjiang Normal University, Zhanjiang 524048, China 4 State Key Laboratory of Genetic Engineering, Institute of Plant Biology, School of Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China
* Author to whom correspondence should be addressed.
Received: 13 December 2012; in revised form: 4 March 2013 / Accepted: 7 March 2013 / Published: 27 March 2013
(This article belongs to the Special Issue Abiotic and Biotic Stress Tolerance Mechanisms in Plants)
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Abstract: With no lysine kinases (WNKs) play important roles in plant growth and development. However, its role in salt and osmotic stress tolerance is unclear. Here, we report that AtWNK8 is mainly expressed in primary root, hypocotyl, stamen and pistil and is induced by NaCl and sorbitol treatment. Compared to the wild-type, the T-DNA knock-out wnk8 mutant was more tolerant to severe salinity and osmotic stresses, as indicated by 27% and 198% more fresh weight in the NaCl and sorbitol treatment, respectively. The wnk8 mutant also accumulated 1.43-fold more proline than the wild-type in the sorbitol treatment. Under NaCl and sorbitol stresses, catalase (CAT) activity in wnk8 mutant was 1.92- and 3.7-times of that in Col-0, respectively. Similarly, under salt and osmotic stress conditions, peroxidase (POD) activities in wnk8 mutant were 1.81- and 1.58-times of that in Col-0, respectively. Taken together, we revealed that maintaining higher CAT and POD activities might be one of the reasons that the disruption of AtWNK8 enhances the tolerance to salt stress, and accumulating more proline and higher activities of CAT and POD might result in the higher tolerance of WNK8 to osmotic stress.
Keywords: AtWNK8; Arabidopsis; salt stress; osmotic stress; proline; catalase; peroxidase

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MDPI and ACS Style

Zhang, B.; Liu, K.; Zheng, Y.; Wang, Y.; Wang, J.; Liao, H. Disruption of AtWNK8 Enhances Tolerance of Arabidopsis to Salt and Osmotic Stresses via Modulating Proline Content and Activities of Catalase and Peroxidase. Int. J. Mol. Sci. 2013, 14, 7032-7047.

AMA Style

Zhang B, Liu K, Zheng Y, Wang Y, Wang J, Liao H. Disruption of AtWNK8 Enhances Tolerance of Arabidopsis to Salt and Osmotic Stresses via Modulating Proline Content and Activities of Catalase and Peroxidase. International Journal of Molecular Sciences. 2013; 14(4):7032-7047.

Chicago/Turabian Style

Zhang, Baige; Liu, Kaidong; Zheng, Yan; Wang, Yingxiang; Wang, Jinxiang; Liao, Hong. 2013. "Disruption of AtWNK8 Enhances Tolerance of Arabidopsis to Salt and Osmotic Stresses via Modulating Proline Content and Activities of Catalase and Peroxidase." Int. J. Mol. Sci. 14, no. 4: 7032-7047.

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