Gold-Coated Iron Composite Nanospheres Targeted the Detection of Escherichia coli
AbstractWe report the preparation and characterization of spherical core-shell structured Fe3O4–Au magnetic nanoparticles, modified with two component self-assembled monolayers (SAMs) consisting of 3–mercaptophenylboronic acid (3–MBA) and 1–decanethiol (1–DT). The rapid and room temperature synthesis of magnetic nanoparticles was achieved using the hydroxylamine reduction of HAuCl4 on the surface of ethylenediaminetetraacetic acid (EDTA)-immobilized iron (magnetite Fe3O4) nanoparticles in the presence of an aqueous solution of hexadecyltrimetylammonium bromide (CTAB) as a dispersant. The reduction of gold on the surface of Fe3O4 nanoparticles exhibits a uniform, highly stable, and narrow particle size distribution of Fe3O4–Au nanoparticles with an average diameter of 9 ± 2 nm. The saturation magnetization value for the resulting nanoparticles was found to be 15 emu/g at 298 K. Subsequent surface modification with SAMs against glucoside moieties on the surface of bacteria provided effective magnetic separation. Comparison of the bacteria capturing efficiency, by means of different molecular recognition agents 3–MBA, 1–DT and the mixed monolayer of 3–MBA and 1–DT was presented. The best capturing efficiency of E. coli was achieved with the mixed monolayer of 3–MBA and 1–DT-modified nanoparticles. Molecular specificity and selectivity were also demonstrated by comparing the surface-enhanced Raman scattering (SERS) spectrum of E. coli-nanoparticle conjugates with bacterial growth media.
- Supplementary File 1:
Supplementary Information (DOC, 2845 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Tamer, U.; Cetin, D.; Suludere, Z.; Boyaci, I.H.; Temiz, H.T.; Yegenoglu, H.; Daniel, P.; Dinçer, İ.; Elerman, Y. Gold-Coated Iron Composite Nanospheres Targeted the Detection of Escherichia coli. Int. J. Mol. Sci. 2013, 14, 6223-6240.
Tamer U, Cetin D, Suludere Z, Boyaci IH, Temiz HT, Yegenoglu H, Daniel P, Dinçer İ, Elerman Y. Gold-Coated Iron Composite Nanospheres Targeted the Detection of Escherichia coli. International Journal of Molecular Sciences. 2013; 14(3):6223-6240.Chicago/Turabian Style
Tamer, Ugur; Cetin, Demet; Suludere, Zekiye; Boyaci, Ismail H.; Temiz, Havva T.; Yegenoglu, Hande; Daniel, Philippe; Dinçer, İlker; Elerman, Yalçın. 2013. "Gold-Coated Iron Composite Nanospheres Targeted the Detection of Escherichia coli." Int. J. Mol. Sci. 14, no. 3: 6223-6240.