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Comparative Analysis of Serum (Anti)oxidative Status Parаmeters in Healthy Persons
Center for Health Protection, National Institute for Public Health and the Environment, Bilthoven, The Netherlands
Faculty of Medical Sciences, Goce Delcev University, Stip 2000, Macedonia
* Author to whom correspondence should be addressed.
Received: 31 December 2012; in revised form: 5 March 2013 / Accepted: 7 March 2013 / Published: 18 March 2013
Abstract: Five antioxidant and two oxidative stress assays were applied to serum samples of 43 healthy males. The antioxidant tests showed different inter-assay correlations. A very good correlation of 0.807 was observed between the ferric reducing ability of plasma (FRAP) and total antioxidant status (TAS) assay and also a fair correlation of 0.501 between the biological antioxidant potential (BAP) and TAS assay. There was no statistically significant correlation between the BAP and FRAP assay. The anti-oxidant assays have a high correlation with uric acid, especially the TAS (0.922) and FRAP assay (0.869). The BAP assay has a much lower and no statistically significant correlation with uric acid (0.302), which makes BAP more suitable for the antioxidant status. The total thiol assay showed no statistically significant correlation with uric acid (0.114). The total thiol assay, which is based on a completely different principle, showed a good and statistically significant correlation with the BAP assay (0.510) and also to the TAS assay, but to a lower and not significant extent (0.279) and not with the FRAP assay (−0.008). The oxy-adsorbent test (OXY) assay has no correlation with any of the other assays tested. The oxidative stress assays, reactive oxygen metabolites (ROM) and total oxidant status (TOS), based on a different principle, do not show a statistically significant correlation with the serum samples in this study. Both assays showed a negative, but not significant, correlation with the antioxidant assays. In conclusion, the ROM, TOS, BAP and TTP assays are based on different principles and will have an additional value when a combination of these assays will be applied in large-scale population studies.
Keywords: oxidation assays; antioxidant assays; total thiol assay; correlation
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Jansen, E.H.; Ruskovska, T. Comparative Analysis of Serum (Anti)oxidative Status Parаmeters in Healthy Persons. Int. J. Mol. Sci. 2013, 14, 6106-6115.
Jansen EH, Ruskovska T. Comparative Analysis of Serum (Anti)oxidative Status Parаmeters in Healthy Persons. International Journal of Molecular Sciences. 2013; 14(3):6106-6115.
Jansen, Eugène H.; Ruskovska, Tatjana. 2013. "Comparative Analysis of Serum (Anti)oxidative Status Parаmeters in Healthy Persons." Int. J. Mol. Sci. 14, no. 3: 6106-6115.