9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers

doi:10.3390/ijms14035723

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Int. J. Mol. Sci. 2013, 14(3), 5723-5733; doi:10.3390/ijms14035723

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9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers

Satish Balasaheb Nimse¹, Keum-Soo Song², Junghoon Kim², Danishmalik Rafiq Sayyed¹ and Taisun Kim^1,*

¹ Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea ² Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, Korea

* Author to whom correspondence should be addressed.

Received: 8 December 2012; in revised form: 1 February 2013 / Accepted: 4 March 2013 / Published: 12 March 2013

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Abstract: A 9G DNAChip obtained by allowing the formation of a self-assembled monolayer (SAM) of oligonucleotides appended with nine consecutive guanines on the chip surface has been applied in the detection of biomarkers. Using a 9G DNAChip, biomarker in the concentration range of 4 pg/mL to 40 fg/mL can be easily differentiated in the buffer matrix. Moreover, it is the first time that a biomarker with a concentration of 40 fg/mL has been detected in a mixture of proteins without use of any signal amplification technique.

Keywords: self-assembly; SAM of ssDNA; biomarker; 9G DNAChip; hybridization; C-reactive protein

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MDPI and ACS Style

Nimse, S.B.; Song, K.-S.; Kim, J.; Sayyed, D.R.; Kim, T. 9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers. Int. J. Mol. Sci. 2013, 14, 5723-5733.

AMA Style

Nimse SB, Song K-S, Kim J, Sayyed DR, Kim T. 9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers. International Journal of Molecular Sciences. 2013; 14(3):5723-5733.

Chicago/Turabian Style

Nimse, Satish B.; Song, Keum-Soo; Kim, Junghoon; Sayyed, Danishmalik R.; Kim, Taisun. 2013. "9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers." Int. J. Mol. Sci. 14, no. 3: 5723-5733.

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