Int. J. Mol. Sci. 2013, 14(3), 5723-5733; doi:10.3390/ijms14035723
Article

9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers

Received: 8 December 2012; in revised form: 1 February 2013 / Accepted: 4 March 2013 / Published: 12 March 2013
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A 9G DNAChip obtained by allowing the formation of a self-assembled monolayer (SAM) of oligonucleotides appended with nine consecutive guanines on the chip surface has been applied in the detection of biomarkers. Using a 9G DNAChip, biomarker in the concentration range of 4 pg/mL to 40 fg/mL can be easily differentiated in the buffer matrix. Moreover, it is the first time that a biomarker with a concentration of 40 fg/mL has been detected in a mixture of proteins without use of any signal amplification technique.
Keywords: self-assembly; SAM of ssDNA; biomarker; 9G DNAChip; hybridization; C-reactive protein
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MDPI and ACS Style

Nimse, S.B.; Song, K.-S.; Kim, J.; Sayyed, D.R.; Kim, T. 9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers. Int. J. Mol. Sci. 2013, 14, 5723-5733.

AMA Style

Nimse SB, Song K-S, Kim J, Sayyed DR, Kim T. 9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers. International Journal of Molecular Sciences. 2013; 14(3):5723-5733.

Chicago/Turabian Style

Nimse, Satish B.; Song, Keum-Soo; Kim, Junghoon; Sayyed, Danishmalik R.; Kim, Taisun. 2013. "9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers." Int. J. Mol. Sci. 14, no. 3: 5723-5733.

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