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Int. J. Mol. Sci. 2013, 14(3), 5650-5667; doi:10.3390/ijms14035650
Article

Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1

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Received: 5 November 2012; in revised form: 12 February 2013 / Accepted: 25 February 2013 / Published: 11 March 2013
(This article belongs to the collection Advances in Proteomic Research)
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Abstract: Analysis of Portuguese wheat (Triticum aestivum L.) landrace ‘Barbela’ revealed the existence of a new x-type high molecular weight-glutenin subunit (HMW-GS) encoded at the Glu-A1 locus, which we named 1Ax1.1. Using one-dimensional and two-dimensional electrophoresis and mass spectrometry, we compared subunit 1Ax1.1 with other subunits encoded at the Glu-A1 locus. Subunit 1Ax1.1 has a theoretical molecular weight of 93,648 Da (or 91,508 Da for the mature protein) and an isoelectric point (pI) of about 5.7, making it the largest and most acidic HMW-GS known to be encoded at Glu-A1. Specific primers were designed to amplify and sequence 2601 bp of the Glu-A1 locus from the ‘Barbela 28’ wheat genome. A very high level of identity was found between the sequence encoding 1Ax1.1 and those encoding other alleles of the locus. The major difference found was an insertion of 36 amino acids in the central repetitive domain.
Keywords: Triticum aestivum L.; wheat; storage proteins; glutenins; HMW-GS; Glu-A1; 1Ax1.1; MALDI-TOF-MS Triticum aestivum L.; wheat; storage proteins; glutenins; HMW-GS; Glu-A1; 1Ax1.1; MALDI-TOF-MS
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ribeiro, M.; Bancel, E.; Faye, A.; Dardevet, M.; Ravel, C.; Branlard, G.; Igrejas, G. Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1. Int. J. Mol. Sci. 2013, 14, 5650-5667.

AMA Style

Ribeiro M, Bancel E, Faye A, Dardevet M, Ravel C, Branlard G, Igrejas G. Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1. International Journal of Molecular Sciences. 2013; 14(3):5650-5667.

Chicago/Turabian Style

Ribeiro, Miguel; Bancel, Emmanuelle; Faye, Annie; Dardevet, Mireille; Ravel, Catherine; Branlard, Gérard; Igrejas, Gilberto. 2013. "Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1." Int. J. Mol. Sci. 14, no. 3: 5650-5667.


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