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MUSTN1 mRNA Abundance and Protein Localization is Greatest in Muscle Tissues of Chinese Meat-Quality Chickens
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, Sichuan, China
Department of Animal and Poultry Sciences 0306, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
* Author to whom correspondence should be addressed.
Received: 3 December 2012; in revised form: 19 January 2013 / Accepted: 31 January 2013 / Published: 8 March 2013
Abstract: The Mustang, Musculoskeletal Temporally Activated Novel-1 Gene (MUSTN1) plays an important role in regulating musculoskeletal development in mammals. We evaluated the developmental and tissue-specific regulation of MUSTN1 mRNA and protein abundance in Erlang Mountainous (EM) chickens. Results indicated that MUSTN1 mRNA/protein was expressed in most tissues with especially high expression in heart and skeletal muscle. The MUSTN1 protein localized to the nucleus in myocardium and skeletal muscle fibers. There were significant differences in mRNA and protein abundance among tissues, ages and between males and females. In conclusion, MUSTN1 was expressed the greatest in skeletal muscle where it localized to the nucleus. Thus, in chickens MUSTN1 may play a vital role in muscle development.
Keywords: Erlang Mountainous chickens; MUSTN1; real-time quantitative PCR; skeletal muscle; western blotting
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Li, J.; Chen, Y.; Wang, Y.-G.; Zhao, X.-L.; Gilbert, E.R.; Liu, Y.-P.; Wang, Y.; Hu, Y.-D.; Zhu, Q. MUSTN1 mRNA Abundance and Protein Localization is Greatest in Muscle Tissues of Chinese Meat-Quality Chickens. Int. J. Mol. Sci. 2013, 14, 5545-5559.
Li J, Chen Y, Wang Y-G, Zhao X-L, Gilbert ER, Liu Y-P, Wang Y, Hu Y-D, Zhu Q. MUSTN1 mRNA Abundance and Protein Localization is Greatest in Muscle Tissues of Chinese Meat-Quality Chickens. International Journal of Molecular Sciences. 2013; 14(3):5545-5559.
Li, Juan; Chen, Yang; Wang, Ya-Gang; Zhao, Xiao-Ling; Gilbert, Elizabeth R.; Liu, Yi-Ping; Wang, Yan; Hu, Yao-Dong; Zhu, Qing. 2013. "MUSTN1 mRNA Abundance and Protein Localization is Greatest in Muscle Tissues of Chinese Meat-Quality Chickens." Int. J. Mol. Sci. 14, no. 3: 5545-5559.