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Int. J. Mol. Sci. 2013, 14(3), 5545-5559; doi:10.3390/ijms14035545
Article

MUSTN1 mRNA Abundance and Protein Localization is Greatest in Muscle Tissues of Chinese Meat-Quality Chickens

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Received: 3 December 2012 / Revised: 19 January 2013 / Accepted: 31 January 2013 / Published: 8 March 2013
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Abstract

The Mustang, Musculoskeletal Temporally Activated Novel-1 Gene (MUSTN1) plays an important role in regulating musculoskeletal development in mammals. We evaluated the developmental and tissue-specific regulation of MUSTN1 mRNA and protein abundance in Erlang Mountainous (EM) chickens. Results indicated that MUSTN1 mRNA/protein was expressed in most tissues with especially high expression in heart and skeletal muscle. The MUSTN1 protein localized to the nucleus in myocardium and skeletal muscle fibers. There were significant differences in mRNA and protein abundance among tissues, ages and between males and females. In conclusion, MUSTN1 was expressed the greatest in skeletal muscle where it localized to the nucleus. Thus, in chickens MUSTN1 may play a vital role in muscle development.
Keywords: Erlang Mountainous chickens; MUSTN1; real-time quantitative PCR; skeletal muscle; western blotting Erlang Mountainous chickens; MUSTN1; real-time quantitative PCR; skeletal muscle; western blotting
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Li, J.; Chen, Y.; Wang, Y.-G.; Zhao, X.-L.; Gilbert, E.R.; Liu, Y.-P.; Wang, Y.; Hu, Y.-D.; Zhu, Q. MUSTN1 mRNA Abundance and Protein Localization is Greatest in Muscle Tissues of Chinese Meat-Quality Chickens. Int. J. Mol. Sci. 2013, 14, 5545-5559.

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