Vascular Endothelial Growth Factor Receptor Family in Ascidians, Halocynthia roretzi (Sea Squirt). Its High Expression in Circulatory System-Containing Tissues
Abstract
:Abbreviation
VEGF | Vascular endothelial growth factor |
VEGFR | Vascular endothelial growth factor receptor |
S. sq. | Sea squirt |
B. schlosseri | Botryllus schlosseri |
H. roretzi | Halocynthia roretzi |
C. intestinalis | Ciona intestinalis |
1. Introduction
2. Results and Discussion
2.1. Isolation of VEGFR-Like Tyrosine Kinase Gene in H. roretzi by Using Degeneration-Primer Method
2.2. The Structure of S. sq VEGFR Is Closer to VEGFR Family than PDGFR Family in Humans
2.3. Amino Acid Sequence Critical for the Intracellular Signaling of Human VEGFR Is Conserved in S. sq VEGFR
2.4. An Intimate Relationship between Expression of S. sq VEGFR mRNA and the Circulatory System in H. roretzi
3. Experimental Section
3.1. Animals
3.2. The Acid Guanidium-Phenol-Chloroform (AGPC) Method
3.3. cDNA Synthesis
3.4. Degenerated Oligonucleotide Primers
(A) Primers for isolation of a VEGFR-like tyrosine kinase gene in H. roretzi
(B) Primers for detection of β-actin mRNA in H. roretzi
3.5. PCR
3.6. Restriction Enzymes
3.7. Histological Analysis
3.8. RT-PCR Detection of S. sq VEGFR mRNA
3.9. Generation of Antibody
3.10. Western Blotting
4. Conclusions
Acknowledgments
Conflict of Interest
References
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Samarghandian, S.; Shibuya, M. Vascular Endothelial Growth Factor Receptor Family in Ascidians, Halocynthia roretzi (Sea Squirt). Its High Expression in Circulatory System-Containing Tissues. Int. J. Mol. Sci. 2013, 14, 4841-4853. https://doi.org/10.3390/ijms14034841
Samarghandian S, Shibuya M. Vascular Endothelial Growth Factor Receptor Family in Ascidians, Halocynthia roretzi (Sea Squirt). Its High Expression in Circulatory System-Containing Tissues. International Journal of Molecular Sciences. 2013; 14(3):4841-4853. https://doi.org/10.3390/ijms14034841
Chicago/Turabian StyleSamarghandian, Saeed, and Masabumi Shibuya. 2013. "Vascular Endothelial Growth Factor Receptor Family in Ascidians, Halocynthia roretzi (Sea Squirt). Its High Expression in Circulatory System-Containing Tissues" International Journal of Molecular Sciences 14, no. 3: 4841-4853. https://doi.org/10.3390/ijms14034841