Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Int. J. Mol. Sci. 2013, 14(2), 3921-3945; doi:10.3390/ijms14023921
Article

Proteome Analysis of Rice (Oryza sativa L.) Mutants Reveals Differentially Induced Proteins during Brown Planthopper (Nilaparvata lugens) Infestation

1,2
, 1,3
, 2
, 2,4
, 4
, 4
, 5
, 6
, 1
, 2
, 1,7
 and 1,*
Received: 17 September 2012; in revised form: 20 January 2013 / Accepted: 22 January 2013 / Published: 15 February 2013
(This article belongs to the Special Issue Abiotic and Biotic Stress Tolerance Mechanisms in Plants)
View Full-Text   |   Download PDF [4056 KB, updated 19 June 2014; original version uploaded 19 June 2014]
Abstract: Although rice resistance plays an important role in controlling the brown planthopper (BPH), Nilaparvata lugens, not all varieties have the same level of protection against BPH infestation. Understanding the molecular interactions in rice defense response is an important tool to help to reveal unexplained processes that underlie rice resistance to BPH. A proteomics approach was used to explore how wild type IR64 and near-isogenic rice mutants with gain and loss of resistance to BPH respond during infestation. A total of 65 proteins were found markedly altered in wild type IR64 during BPH infestation. Fifty-two proteins associated with 11 functional categories were identified using mass spectrometry. Protein abundance was less altered at 2 and 14 days after infestation (DAI) (T1, T2, respectively), whereas higher protein levels were observed at 28 DAI (T3). This trend diminished at 34 DAI (T4). Comparative analysis of IR64 with mutants showed 22 proteins that may be potentially associated with rice resistance to the brown planthopper (BPH). Ten proteins were altered in susceptible mutant (D1131) whereas abundance of 12 proteins including S-like RNase, Glyoxalase I, EFTu1 and Salt stress root protein “RS1” was differentially changed in resistant mutant (D518). S-like RNase was found in greater quantities in D518 after BPH infestation but remained unchanged in IR64 and decreased in D1131. Taken together, this study shows a noticeable level of protein abundance in the resistant mutant D518 compared to the susceptible mutant D1131 that may be involved in rendering enhanced level of resistance against BPH.
Keywords: rice resistance; brown planthopper; proteomics; S-like RNase; molecular docking rice resistance; brown planthopper; proteomics; S-like RNase; molecular docking
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Sangha, J.S.; Chen, Y.H.; Kaur, J.; Khan, W.; Abduljaleel, Z.; Alanazi, M.S.; Mills, A.; Adalla, C.B.; Bennett, J.; Prithiviraj, B.; Jahn, G.C.; Leung, H. Proteome Analysis of Rice (Oryza sativa L.) Mutants Reveals Differentially Induced Proteins during Brown Planthopper (Nilaparvata lugens) Infestation. Int. J. Mol. Sci. 2013, 14, 3921-3945.

AMA Style

Sangha JS, Chen YH, Kaur J, Khan W, Abduljaleel Z, Alanazi MS, Mills A, Adalla CB, Bennett J, Prithiviraj B, Jahn GC, Leung H. Proteome Analysis of Rice (Oryza sativa L.) Mutants Reveals Differentially Induced Proteins during Brown Planthopper (Nilaparvata lugens) Infestation. International Journal of Molecular Sciences. 2013; 14(2):3921-3945.

Chicago/Turabian Style

Sangha, Jatinder S.; Chen, Yolanda H.; Kaur, Jatinder; Khan, Wajahatullah; Abduljaleel, Zainularifeen; Alanazi, Mohammed S.; Mills, Aaron; Adalla, Candida B.; Bennett, John; Prithiviraj, Balakrishnan; Jahn, Gary C.; Leung, Hei. 2013. "Proteome Analysis of Rice (Oryza sativa L.) Mutants Reveals Differentially Induced Proteins during Brown Planthopper (Nilaparvata lugens) Infestation." Int. J. Mol. Sci. 14, no. 2: 3921-3945.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert