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Proteome Analysis of Rice (Oryza sativa L.) Mutants Reveals Differentially Induced Proteins during Brown Planthopper (Nilaparvata lugens) Infestation
Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines
Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia B2N 5E3, Canada
Department of Plant and Soil Sciences, University of Vermont, 63 Carrigan Drive, Burlington, VT 05405, USA
Genome Research Chair Unit, Biochemistry Department, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia
Crops and Livestock Research Center, Agriculture and Agri-Food Canada, 440 University Ave., Charlottetown, Prince Edward Island C1A4N6, Canada
Department of Entomology, College of Agriculture, University of the Philippines, Los Banos, Laguna 4031, Philippines
Georgetown University Medical Center, Department of Microbiology and Immunology, Washington, DC 20057, USA
* Author to whom correspondence should be addressed.
Received: 17 September 2012; in revised form: 20 January 2013 / Accepted: 22 January 2013 / Published: 15 February 2013
Abstract: Although rice resistance plays an important role in controlling the brown planthopper (BPH), Nilaparvata lugens, not all varieties have the same level of protection against BPH infestation. Understanding the molecular interactions in rice defense response is an important tool to help to reveal unexplained processes that underlie rice resistance to BPH. A proteomics approach was used to explore how wild type IR64 and near-isogenic rice mutants with gain and loss of resistance to BPH respond during infestation. A total of 65 proteins were found markedly altered in wild type IR64 during BPH infestation. Fifty-two proteins associated with 11 functional categories were identified using mass spectrometry. Protein abundance was less altered at 2 and 14 days after infestation (DAI) (T1, T2, respectively), whereas higher protein levels were observed at 28 DAI (T3). This trend diminished at 34 DAI (T4). Comparative analysis of IR64 with mutants showed 22 proteins that may be potentially associated with rice resistance to the brown planthopper (BPH). Ten proteins were altered in susceptible mutant (D1131) whereas abundance of 12 proteins including S-like RNase, Glyoxalase I, EFTu1 and Salt stress root protein “RS1” was differentially changed in resistant mutant (D518). S-like RNase was found in greater quantities in D518 after BPH infestation but remained unchanged in IR64 and decreased in D1131. Taken together, this study shows a noticeable level of protein abundance in the resistant mutant D518 compared to the susceptible mutant D1131 that may be involved in rendering enhanced level of resistance against BPH.
Keywords: rice resistance; brown planthopper; proteomics; S-like RNase; molecular docking
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Sangha, J.S.; Chen, Y.H.; Kaur, J.; Khan, W.; Abduljaleel, Z.; Alanazi, M.S.; Mills, A.; Adalla, C.B.; Bennett, J.; Prithiviraj, B.; Jahn, G.C.; Leung, H. Proteome Analysis of Rice (Oryza sativa L.) Mutants Reveals Differentially Induced Proteins during Brown Planthopper (Nilaparvata lugens) Infestation. Int. J. Mol. Sci. 2013, 14, 3921-3945.
Sangha JS, Chen YH, Kaur J, Khan W, Abduljaleel Z, Alanazi MS, Mills A, Adalla CB, Bennett J, Prithiviraj B, Jahn GC, Leung H. Proteome Analysis of Rice (Oryza sativa L.) Mutants Reveals Differentially Induced Proteins during Brown Planthopper (Nilaparvata lugens) Infestation. International Journal of Molecular Sciences. 2013; 14(2):3921-3945.
Sangha, Jatinder S.; Chen, Yolanda H.; Kaur, Jatinder; Khan, Wajahatullah; Abduljaleel, Zainularifeen; Alanazi, Mohammed S.; Mills, Aaron; Adalla, Candida B.; Bennett, John; Prithiviraj, Balakrishnan; Jahn, Gary C.; Leung, Hei. 2013. "Proteome Analysis of Rice (Oryza sativa L.) Mutants Reveals Differentially Induced Proteins during Brown Planthopper (Nilaparvata lugens) Infestation." Int. J. Mol. Sci. 14, no. 2: 3921-3945.