Rsk2 Knockdown in PC12 Cells Results in Sp1 Dependent Increased Expression of the Gria2 Gene, Encoding the AMPA Receptor Subunit GluR2
Abstract
:1. Introduction
2. Results and Discussion
2.1. Rsk2 Knockdown of PC12 Cells
2.2. Immunohistochemical Analysis of RSK2
2.3. Rsk2 Silencing Increased ERK1/2 MAPK Phosphorylation
2.4. Rsk2 Knockdown Results in up Regulation of Gria2 Gene (Encoding GluR2) Expression
2.5. Inhibition of GluR2 over Expression in Rsk2 Knockdown PC12 Cells by U0126
2.6. Involvement of Sp1 in the up Regulation of GluR2 Expression in Rsk2 Knockdown PC12 Cells
2.7. Confirmation of Significantly Increased Sp1 Phosphorylation in the Hippocampus of Rsk2-KO Mice
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Transfection of PC12 Cells with shRNA Vectors
4.3. siRNA Transfection
4.4. Cell Cultures Treatment with the MEK Inhibitor
4.5. Western Blot Analysis
4.6. Real-Time QRT-PCR Analysis
4.7. Immunofluorescence
4.8. Statistical Analysis
5. Conclusions
Acknowledgements
Conflict of Interest
References
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Mehmood, T.; Schneider, A.; Pannetier, S.; Hanauer, A. Rsk2 Knockdown in PC12 Cells Results in Sp1 Dependent Increased Expression of the Gria2 Gene, Encoding the AMPA Receptor Subunit GluR2. Int. J. Mol. Sci. 2013, 14, 3358-3375. https://doi.org/10.3390/ijms14023358
Mehmood T, Schneider A, Pannetier S, Hanauer A. Rsk2 Knockdown in PC12 Cells Results in Sp1 Dependent Increased Expression of the Gria2 Gene, Encoding the AMPA Receptor Subunit GluR2. International Journal of Molecular Sciences. 2013; 14(2):3358-3375. https://doi.org/10.3390/ijms14023358
Chicago/Turabian StyleMehmood, Tahir, Anne Schneider, Solange Pannetier, and André Hanauer. 2013. "Rsk2 Knockdown in PC12 Cells Results in Sp1 Dependent Increased Expression of the Gria2 Gene, Encoding the AMPA Receptor Subunit GluR2" International Journal of Molecular Sciences 14, no. 2: 3358-3375. https://doi.org/10.3390/ijms14023358