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Int. J. Mol. Sci. 2013, 14(2), 3065-3077; doi:10.3390/ijms14023065
Article

Cytotoxicity and Genotoxicity of Ceria Nanoparticles on Different Cell Lines in Vitro

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Received: 29 November 2012; in revised form: 4 January 2013 / Accepted: 21 January 2013 / Published: 1 February 2013
(This article belongs to the Special Issue Bioactive Nanoparticles 2012)
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Abstract: Owing to their radical scavenging and UV-filtering properties, ceria nanoparticles (CeO2-NPs) are currently used for various applications, including as catalysts in diesel particulate filters. Because of their ability to filter UV light, CeO2-NPs have garnered significant interest in the medical field and, consequently, are poised for use in various applications. The aim of this work was to investigate the effects of short-term (24 h) and long-term (10 days) CeO2-NP exposure to A549, CaCo2 and HepG2 cell lines. Cytotoxicity assays tested CeO2-NPs over a concentration range of 0.5 μg/mL to 5000 μg/mL, whereas genotoxicity assays tested CeO2-NPs over a concentration range of 0.5 μg/mL to 5000 μg/mL. In vitro assays showed almost no short-term exposure toxicity on any of the tested cell lines. Conversely, long-term CeO2-NP exposure proved toxic for all tested cell lines. NP genotoxicity was detectable even at 24-h exposure. HepG2 was the most sensitive cell line overall; however, the A549 line was most sensitive to the lowest concentration tested. Moreover, the results confirmed the ceria nanoparticles’ capacity to protect cells when they are exposed to well-known oxidants such as H2O2. A Comet assay was performed in the presence of both H2O2 and CeO2-NPs. When hydrogen peroxide was maintained at 25 μM, NPs at 0.5 μg/mL, 50 μg/mL, and 500 μg/mL protected the cells from oxidative damage. Thus, the NPs prevented H2O2-induced genotoxic damage.
Keywords: ceria nanoparticles; short-term exposure cytotoxicity; long-term exposure cytotoxicity; genotoxicity ceria nanoparticles; short-term exposure cytotoxicity; long-term exposure cytotoxicity; genotoxicity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

De Marzi, L.; Monaco, A.; De Lapuente, J.; Ramos, D.; Borras, M.; Di Gioacchino, M.; Santucci, S.; Poma, A. Cytotoxicity and Genotoxicity of Ceria Nanoparticles on Different Cell Lines in Vitro. Int. J. Mol. Sci. 2013, 14, 3065-3077.

AMA Style

De Marzi L, Monaco A, De Lapuente J, Ramos D, Borras M, Di Gioacchino M, Santucci S, Poma A. Cytotoxicity and Genotoxicity of Ceria Nanoparticles on Different Cell Lines in Vitro. International Journal of Molecular Sciences. 2013; 14(2):3065-3077.

Chicago/Turabian Style

De Marzi, Laura; Monaco, Antonina; De Lapuente, Joaquin; Ramos, David; Borras, Miquel; Di Gioacchino, Mario; Santucci, Sandro; Poma, Anna. 2013. "Cytotoxicity and Genotoxicity of Ceria Nanoparticles on Different Cell Lines in Vitro." Int. J. Mol. Sci. 14, no. 2: 3065-3077.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert