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Int. J. Mol. Sci. 2013, 14(2), 2334-2354; doi:10.3390/ijms14022334
Abstract: Inhibition of apoptosis leads to activation of cell survival factors (e.g., AKT) causes continuous cell proliferation in cancer. Apoptosis, the major form of cellular suicide, is central to various physiological processes and the maintenance of homeostasis in multicellular organisms. A number of discoveries have clarified the molecular mechanism of apoptosis, thus clarifying the link between apoptosis and cell survival factors, which has a therapeutic outcome. Induction of apoptosis and inhibition of cell survival by anticancer agents has been shown to correlate with tumor response. Cellular damage induces growth arrest and tumor suppression by inducing apoptosis, necrosis and senescence; the mechanism of cell death depends on the magnitude of DNA damage following exposure to various anticancer agents. Apoptosis is mainly regulated by cell survival and proliferating signaling molecules. As a new therapeutic strategy, alternative types of cell death might be exploited to control and eradicate cancer cells. This review discusses the signaling of apoptosis and cell survival, as well as the potential contribution of marine bioactive compounds, suggesting that new therapeutic strategies might follow.
Apoptosis is critically important for the survival of multicellular organisms . Apoptosis triggered by exogenous and endogenous stimuli, such as ultraviolet radiation, oxidative stress and genotoxic chemicals, is a crucial phenomenon within biological systems. Cell death could be caused by necrosis and apoptosis. The extrinsic and intrinsic pathways represent the two major well-studied apoptotic processes [2,3]. The extrinsic pathway mediated by the sub group of Tumor Necrosis Factor receptors (TNF-R) super family that includes TNF-R, Fas and Tumor necrosis factor receptor is related to apoptosis inducing ligand (TRAIL). Activation of these so-called death receptors leads to the recruitment and activation of initiator caspases, such as caspases 8 and 10. The process involves the formation and activation of complexes, such as the death inducing signaling complex (DISC). This leads to the activation of an effector caspase, typically caspase 3. The active caspase 3 responsible for the cleavage of number of death substrates leads to the well-known characteristic hallmarks of apoptotic cell, including DNA fragmentation, nuclear fragmentation, membrane blebbing and other morphological and biochemical changes. More evidences suggest that greater complexity and diversity in the extrinsic pathways also involves the cross-activation of other apoptotic pathways, such as the intrinsic apoptotic, as well as necrotic sub pathways [2,4].
The mitochondrial pathway of apoptosis is initiated by a variety of upstream stimuli and tightly regulated by various factors, including pro and anti apoptotic proteins of the Bcl-2 family, as well as the phosphatidyl inositol 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) pathway [5,6]. The PI3K/AKT/mTOR signaling cascade belongs to the critical survival programs that are typically over activated in human cancers, promoting cell survival by inhibiting the apoptosis . The PI3K signaling network diversifies into many distinct downstream branches, one of which leads to the activation of mTOR . In addition, intricate interactions between distinct kinase of survival networks had been described. Since small-molecule inhibitors that block PI3K/AKT/mTOR signaling are currently undergoing clinical evaluation in early trials, there is much interest to understand how these inhibitors interfere with intracellular signaling pathways, for example, mitochondria-mediated apoptosis. There are number of reviews evidencing apoptosis, but this paper gives a short overview on PI3K/AKT regulation and an apoptosis discussion about some marine bioactive compounds on these aspects.
2. Cell Survival
2.1. Survival Signaling via PI3K/AKT
AKT plays an important role in cell survival . The PI3K/AKT pathway represents a key signal transduction pathway that mediates cell growth and blocks apoptosis . Increased activation of this survival cascade is a characteristic feature of a large variety of human malignancies and has been associated with carcinogenesis . Growth factors binding to their corresponding receptors results in the activation of this survival cascade in order to regulate the intracellular signaling that supports proliferation and survival. Activation of growth factor receptors results in the phosphorylation of receptor tyrosine kinases that resides within the plasma membrane. This, in turn, activates the whole cascade of the PI3K/AKT pathway. This signaling is antagonized by the tumor suppressor gene phosphatase and tensin homologue deleted on chromosome 10 (PTEN), which acts as both a lipid and a protein phosphatase . PTEN dephosphorylates PIP3 in to PIP2, thereby negatively regulating PI3K/AKT signal transduction.
2.2. Role of AKT in Cell Survival
AKT acts as a cell survival factor by regulating apoptotic signal transduction directly or indirectly . AKT has been reported to directly interfere with cell death pathways by phosphorylating key apoptosis-regulatory proteins, which, in turn, results in a shift within the ratio of pro- and anti-apoptotic proteins towards the inhibition of cell death (Figure 1).
Abundant evidence implicates the PI3K/AKT pathway in the development and progression of multiple tumors. AKT is activated in response to many growth factors, hormones and cytokines. Binding the growth factor to its receptor triggers intrinsic tyrosine kinase phosphorylation, subsequently activating IRS (insulin receptor substrate) and PI3K (Figure 2).
Activated PI3K generates phosphatidylinositol-3,4,5-triphosphate, which recruits phosphatidylinositol-dependent kinase (PDK) and AKT serine/threonine kinase at the plasma membrane, resulting in phosphorylation of AKT. The central regulatory mechanism is cell survival. BAD is a pro-apoptotic protein of the Bcl-2 family. AKT could phosphorylate BAD on Ser136, which makes BAD dissociate from the Bcl-2/Bcl-X complex and lose the pro-apoptotic function. Akt could also activate NF-κB via regulating IκB kinase (IKK), thus resulting in transcription of pro-survival genes. Omi/high temperature requirement proteinA2 (HtrA2), a mitochondrial serine protease, is released from the mitochondria to the cytosol during apoptosis. AKT phosphorylates Omi/HtrA2, thereby inhibiting apoptosis . In addition, the multi-domain Bcl-2 protein Bax has been shown to be phosphorylated by AKT in a dependent manner at serine residue 184 [11,12]. AKT mediated phosphorylation of Bax leads to changes in the conformation of Bax and blocks its activation. Besides, Bim (Bcl-2 interacting mediator of cell death) is a BH3 (Bcl-2 homology domain) protein phosphorylated at serine residue, i.e., Ser87 , by AKT. While the phosphorylation of proapoptotic Bcl-2 proteins, Bax and Bim, reduces their proapoptotic potential, AKT mediated phosphorylation of some antiapoptotic factors, such as XIAP (X-linked inhibitor of apoptosis protein) and Mcl-1 (induced myeloid leukemia cell differentiation protein), decrease their antiapoptotic properties by decreasing their protein stability. Accordingly, phosphorylation of XIAP and Mcl-1 by AKT promotes the degradation of these proteins via the proteasomal machinery, resulting in reduction of XIAP and Mcl-1protein expression [14,15].
Apart from the direct interference of AKT with cell death signaling pathways via phosphorylation of key signal transduction molecules, AKT has also been reported to interfere with cell death programs indirectly via the phosphorylation of transcription factors. This mechanism applies to the transcription factor FOXO3 (Forkhead box O3). It belongs to the O subclass of the forkhead family of transcription factors, which are characterized by a distinct forkhead DNA-binding domain. FOXO3 transcription factors, such as FOXO1, FOXO3a, FOXO4 and FOXO6, are involved in the apoptotic mechanism. FOXO3 upregulates Bim and Noxa gene expression [16,17]. FOXO3 action is inhibited by phosphorylation of AKT in the PI3K signaling pathway (Figure 1). AKT mediated activation of the transcription factor NF-κB transactivates wide range of antiapoptotic NF-κB target genes, including inhibitor of apoptosis (IAP) proteins, Bcl-XL and Bcl-2 . PI3K/AKT signaling represents a key regulatory mechanism to control the activity of pro- and anti-apoptotic Bcl-2 family proteins. Accordingly, small-molecule inhibitors of PI3K/AKT signaling reported that the balance between pro- and anti-apoptotic proteins towards apoptosis decrease the expression of Mcl-1 and increase phosphorylation of Bim [19–22]. Phorbol-12-myristate-13-acetate-induced protein 1 is also known as Noxa, a pro-apoptotic member of the Bcl-2 protein family. Increased expression of Noxa may promote mitochondrial apoptosis by directly binding to Mcl-1, thereby antagonizing the antiapoptotic function of Mcl-1 .
3.1. Mechanism of Apoptosis
The process of programmed cell death, or apoptosis, is generally characterized by distinct morphological characteristics and energy-dependent biochemical mechanisms. Impairment of this native defense mechanism promotes aberrant cellular proliferation and the accumulation of genetic defects, ultimately resulting in tumorigenesis . There are two pathways triggered during apoptosis: the intrinsic (mitochondrial) and extrinsic (death receptor) mediated pathway . This apoptotic pathway is centrally regulated by upstream activators. Among these are PI3K/AKT/mTOR signaling, proapoptotic proteins of the Bcl-2 family, cellular stress stimuli and hypoxia . The intrinsic pathway of apoptosis, permeabilization of outer mitochondrial membrane, is associated with the release of mitochondrial proteins from the intermembrane space into the cytosol, such as cytochrome c, second mitochondrial activator of caspases (Smac) and apoptosis inducing factor (AIF). Cytochrome c promotes the aggregation of caspase-9 together with Apaf-1 in the cytosol to form a multi-protein complex called an apoptosome, which results in caspase-9 activation. The release of Smac from the mitochondrial intermembrane space into the cytosol promotes apoptosis by binding to the inhibitor of apoptosis (IAP) proteins . p53 is considered to be “a cellular gatekeeper for growth and division” by controlling critical cell cycle checkpoints . The p53 mediates apoptosis through activation of APO-1/Fas and other death receptors and/or up- and down-regulation of Bax and Bcl-2, respectively [28,29]. p53 is activated by various stress conditions, including radiation. Increased production of reactive oxygen species (ROS) under stress, which activates stress responsive pathways (p38MAPK), thereby promotes apoptosis [30–32].
3.2. Intrinsic Pathway
The intrinsic apoptotic pathway is characterized by membrane permeability that causes mitochondrial swelling, rupture of the outer membrane and release of proapoptotic factors from the intermembranous space. This is achieved by (1) an opening of the permeability transition pore and (2) an increase of the Bax/Bcl-2 ratio 3. The intrinsic signaling pathways mediating apoptosis involves the absence of certain growth factors, hormones and cytokines. Apoptotic stimuli include radiation, drugs, toxins, hypoxia, hyperthermia, viral infections and free radicals. All of these stimuli cause changes in the inner mitochondrial membrane that result in an opening of the mitochondrial permeability transition (MPT) pore and release of two main groups of normally sequestered pro-apoptotic proteins from the intermembrane space into cytosol . The first group consists of cytochrome c, Smac/DIABLO and the serine protease, HtrA2/Omi [34–36]. Cytochrome c binds and activates Apaf-1, forming an “apoptosome” [37,38], adenosine tri phosphate (ATP), also required for the activation of apoptosome complex, which in turn activates procaspase-9 into active caspase-9. DIABLO is also known as SMAC (second mitochondria-derived activator of caspases), and HtrA2/Omi are reported to promote apoptosis by inhibiting IAP (inhibitors of apoptosis proteins) activity [37,39].
The second group of pro-apoptotic proteins, AIF (apoptosis-inducing factor), endonuclease G and CAD (caspase-activated DNase), are released from the mitochondria during apoptosis. AIF, a mitochondrion-localized flavoprotein, triggers chromatin condensation and DNA degradation during apoptosis. AIF translocates to the nucleus and causes DNA fragmentation into 50–300 kb pieces and condensation of peripheral nuclear chromatin [40,41]. This early form of nuclear condensation is referred to as “stage I” condensation . Endonuclease G is a mitochondrial enzyme that also translocates to the nucleus, where it cleaves nuclear chromatin to produce oligonucleosomal DNA fragments . AIF and endonuclease G both function in a caspase-independent manner. CAD is an endonuclease released from the mitochondria and translocates to the nucleus, causing oligonucleosomal DNA fragmentation . This later and more pronounced chromatin condensation is referred to as “stage II” condensation . The control and regulation of mitochondrial apoptotic events occur through members of the Bcl-2 family of proteins . The tumor suppressor protein p53 also plays a critical role in regulation of the Bcl-2 family of proteins .
The discovery of Bcl-2 did not drive cell proliferation, as for previously characterized oncogenes, but promotes cell survival in tumorigenesis [47,48]. The Bcl-2 gene was originally identified at the chromosomal breakpoint of the translocation of chromosome 18 to 14 in follicular non-Hodgkin lymphoma (NHL) . Increased expression of Bcl-2 causes resistance to chemotherapeutic drugs and radiation therapy, while decreasing Bcl-2 expression may promote apoptotic responses to anticancer drugs [50,51]. The Bcl-2 family includes proapoptotic members, such as Bax, Bak, Bad, Bcl-Xs, Bid, Bik and Bim and antiapoptotic members, such as Bcl-2, Bcl-XL, Bcl-W, Bfl-1 and Mcl-1 [52,53]. Antiapoptotic Bcl-2 members act as repressors of apoptosis by blocking the release of cytochrome c, whereas proapoptotic members act as promoters . Following a death signal, proapoptotic proteins undergo post translational modifications that include dephosphorylation and cleavage, resulting in their activation and translocation to the mitochondria, leading to apoptosis . BH3-only molecules require multi-domain BH3 proteins (Bax, Bak) to exert their intrinsic proapoptotic activity [47,54,55].
In response to apoptotic stimuli, cytochrome c, released into the cytosol, interacts with Apaf-1, leading to the activation of caspase-9 proenzymes [56,57]. Active caspase-9 then activates caspase-3, which subsequently activates the rest of the caspase cascade and leads to apoptosis. Activated caspases lead to the cleavage of nuclear lamin and breakdown of the nucleus through caspase-3 .
3.3. Death Receptor Pathway
Death receptors are cell surface receptors that transmit apoptosis signals initiated by their specific “death ligands”. The cell surface death receptors belong to the superfamily of tumor necrosis factor receptors (TNF-R) and are activated by TNF family ligands. This pathway comprises several protein members, including the death receptors, the membrane-bound FasL, the Fas complexes, the Fas-associated death domain and caspases 8 and 10, which ultimately activate the rest of the downstream caspases, leading to apoptosis. Activation of the extrinsic pathway is initiated with the ligation of cell surface receptors, called death receptors (DRs). Fas is a member of the tumor necrosis factor receptor superfamily and is also called Apo-1 or CD95. Other TNF receptors include TNF R1, DR3 (Apo 2), DR4 (tumor necrosis factor related apoptosis-inducing ligand receptor 1 (TRAIL R1), DR5 (TRAIL R2) and DR6 . Fas signaling plays an important role in immune surveillance of transformed or virus infected cells and in the removal of self reactive lymphocytes. Therefore, defects in this pathway have been implicated in many malignancies and autoimmune diseases [60,61].
The Fas ligand and Fas system is mainly recognized for its death-related functions, but it is also involved in several proliferative and inflammatory signaling pathways that are not well defined . Fas ligand (FasL or CD95L) belongs to the tumor necrosis factor (TNF) family. It binds with its receptor to form Fas death-inducing signaling complex (DISC), contains the adaptor protein Fas-associated death domain protein and caspases 8 and 10 and leads to activation of caspase 8, which in turn activates the rest of the downstream caspases. In some cells, the activation of caspase 8 may be the only requirement to execute death, while in other cell types, caspase 8 interacts with the intrinsic apoptotic pathway by cleaving Bid (a proapoptotic member of the Bcl-2 family), activating the release of cytochrome c .
Alterations in the extrinsic pathway lead to malignant transformation, as mutations or deletions of the Fas gene has been found in some hematologic malignancies [61,64]. Regulators of the pathway include transcription factor NF-κB and activating protein 1 that regulate the FasL gene, because it is a transcriptionally inactive gene . The extrinsic pathway of apoptosis is abrogated through several mechanisms, including the upregulation of the inhibitors of apoptosis proteins, such as cIAP or XIAP. Smac/DIABLO, a mitochondrial protein and negative regulator of XIAP, enhances apoptosis by binding with XIAP, thereby preventing binding to caspases. Other inhibitors of the pathway include FAP-1 (Fibroblast activation protein), Fas-associated-death-domain-protein, like interleukin-1, converts enzyme-like inhibitory proteins and the soluble decoy receptors, such as DcR3, TRAIL R-3/DcR1 and TRAIL R-4/ DcR2. These decoy receptors are antagonized by the stimulation of Fas ligand [65,66].
3.4. Final Pathway
The final pathway leads to activation of a family of cysteine aspartyl proteases (caspases) . Caspases are a family of cysteine proteases, which contain cysteine residue at their active site and cleave their substrate at a position next to aspartate residue. The entire group of mammalian caspases is divided into three different groups on the basis of their prodomains and specific function in different pathways, including inflammatory, development and apoptotic pathways . Caspases, totaling 14 family members, are synthesized as inactive zymogens, which have been proteolytically cleaved at two (or three in some cases) aspartate residues to generate the active mature enzyme. The generations of active caspases interact with specific adapter molecules to facilitate their own auto processing. Active initiator caspases, in turn, cleave and activate the downstream “executioner” caspases. These then cleave their target substrates to orchestrate the proteolytic dismantling of the cell [68,69]. Not all caspases are involved in apoptosis; caspases-3, -6, -7, -8 and -9  are well described. The intrinsic and extrinsic apoptotic pathways converge to caspase-3, which cleaves the inhibitor of the caspase-activated deoxyribonuclease, and the caspase-activated deoxyribonuclease becomes active, leading to nuclear apoptosis. The downstream caspases induce cleavage of protein kinases, cytoskeletal proteins, DNA repair proteins, inhibitory subunits of endonuclease and, finally, destruction of “housekeeping” cellular functions. Caspases also affect cytoskeletal structure, cell cycle regulation and signaling pathways, ultimately leading to the morphologic manifestations of apoptosis, such as DNA condensation and fragmentation and membrane blebbing . The intrinsic pathways are triggered by various extracellular and intracellular stresses, such as growth factor withdrawal and hypoxia; DNA damage and oncogene induction signals that are transduced in response to these stresses convert mainly on mitochondria. A series of biochemical events induced during apoptosis results in the permeabilization of the outer mitochondrial membrane, the release of cytochrome c and other proapoptotic molecules, the formation of apoptotic protease activating factor 1, caspase-9 and caspase-3 activation.
3.5. Biochemical Characteristics of Apoptosis
Apoptotic cells exhibit several biochemical modifications, such as protein cleavage, protein cross-linking, DNA breakdown and phagocytic recognition that together result in the distinctive structural pathology described previously . Caspases are widely expressed as inactive proenzyme forms in most cells and, once activated, can often activate other procaspases, allowing initiation of the protease cascade. With this proteolytic cascade, one caspase can activate other caspases, amplifying the apoptotic signaling pathway and, thus, leading to rapid cell death. Caspases are able to cleave proteins at aspartic acid residues, although different caspases have different specificities involving recognition of neighboring amino acids. Once caspases are initially activated, there seems to be an irreversible commitment towards cell death. Caspases are identified and broadly categorized into initiators (caspase-2,-8,-9,-10), effectors or executioners (caspase-3,-6,-7) and inflammatory caspases (caspase-1,-4,-5) [73,74].
The other caspases that have been identified include caspase-11, which has been reported to regulate apoptosis and cytokine maturation during septic shock, caspase-12, which mediates endoplasmic-specific apoptosis and cytotoxicity by amyloid-β, caspase-13, which has been suggested in bovine gene, and caspase-14, which is highly expressed in embryonic tissues, but not in adult tissues [75–78]. Extensive protein cross-linking is another characteristic of apoptotic cells achieved through the expression and activation of tissue transglutaminase . DNA breakdown by Ca2+ and Mg2+ dependent endonucleases also occur, resulting in DNA fragments of 180 to 200 base pairs. With the terminal transferase mediated DNA nick end labeling (TUNEL) assay, cells containing DNA strand breaks become visualized by fluorescent microscope . A characteristic “DNA ladder” was visualized by agarose gel electrophoresis with ethidium bromide staining in ultraviolet illumination. Another biochemical feature is the expression of cell surface markers that result in the early phagocytic recognition of apoptotic cells by adjacent cells, permitting quick phagocytosis with minimal compromise to the surrounding tissue. It is achieved by the movement of the normal inward facing phosphatidylserine of the cell’s lipid bilayer to expression on the outer layers of the plasma membrane . Although externalization of phosphatidylserine is a well known recognition ligand for phagocytes on the surface of the apoptotic cell, studies showed that other proteins are also exposed on the cell surface during apoptotic cell clearance. These include Annexin I and calreticulin. Annexin V is a recombinant phosphatidylserine-binding protein that interacts strongly and specifically with phosphatidylserine residues and could be used for the detection of apoptosis . Calreticulins are protein that binds to an LDL-receptor related protein on the engulfing cell and has suggested cooperation with phosphatidylserine as a recognition signal during apoptosis .
Chemoprevention, a relatively new and promising strategy to prevent cancer, is defined as the use of natural dietary compounds or synthetic substances to block, inhibit, reverse or retard the process of carcinogenesis . Cancer development, a dynamic and long-term process, involves many complex factors with a stepwise progression that ultimately leads to metastasis, an uncontrolled spreading and growth of cancerous cells throughout the body. Large-scale clinical studies have demonstrated treatment for breast cancer with the efficacy of using tamoxifen, raloxifene, both estrogen receptor antagonists and fenretinide, a synthetic retinoid [84–86]. Chemotherapy aims to kill cancer cells in the hope of preventing further cancer progression. Chemoprevention, on the other hand, involves administering non-toxic agents to individuals who may be at an increased risk for cancer. Moreover, surgical and traditional therapeutic approaches (chemotherapy and radiation) are, at present, unable to control most cancer types. Thus, the development of new chemopreventive strategies is required . Chemopreventive compounds are classified into two major groups: (1) blocking agents, which prevent carcinogens from reaching or reacting with critical target sites and (2) suppressing agents, which stop the evolution of pre-neoplastic processes. Given that the initiation and progression phases are relatively transient and irreversible events, it seems that chemopreventive agents should intervene at the prodromal promotion phase. Three decades of research suggest that chemoprevention is a promising strategy to reduce the incidence of cancer, both in well-defined high-risk groups and in the general population [88–90].
Focus has been on the molecular basis of chemopreventive potential of natural compounds, with special emphasis on their effects in cellular signaling molecules as a target. To study the biological effects of phytochemicals at the cellular level provides the molecular basis for their function and helps to establish more potent chemopreventive agents. Many studies have been carried out to find cancer chemotherapeutic agents from edible and natural resources, such as fruits, vegetables and terrestrial plants [91,92]. The marine environment represents a relatively untapped source of functional ingredients. The marine derived bioactive compounds also have important sources for dietary supplements, and a number of them are potentially active.
The Potential Effect of Marine Bioactive Compounds on Cancer Cell Survival and Apoptosis
Nature has been an important source of novel anti-cancer drug leads over the past 25 years , with increasing numbers of new compounds sourced from the marine environment . The chemical and biological diversity of the marine environments are an immeasurable and extraordinary resource for the discovery of new anticancer drugs. Approximately 22,000 natural products of marine origin were discovered so far, whereas 131,000 terrestrial natural products exist . Natural compounds remain a high output source of promising chemotherapeutic or chemopreventive agents in current cancer research [96,97].
Marine organisms, including sponges , sponge-microbe symbiotic association , gorgonian , actinomycetes  and soft coral , have been widely explored for potential anticancer agents. The effects of marine nutraceuticals on apoptotic pathways, signaling pathways and/or different targets in cancer mean that they could be helpful starting points in the design and development of novel cancer preventive agents. Of great importance, aberrant NF-κB regulation and AKT activation has been observed in many cancers. To prevent the development and progression of cancers, the strategy should target the cell signaling pathways in cancer. Aberrant regulation of NF-κB and the signaling pathways that control its activity are involved in cancer development and progression, as well as in drug resistance, especially during chemotherapy and radiotherapy . Blocking NF-κB could cause tumor cells to cease proliferation or become more sensitive to the action of antitumor agents . Changes in Akt activator expression has been observed in human precancerous tissues that might be targeted for chemoprevention . Thus far, several chemopreventive agents have shown their various activities in the inhibition of carcinogenesis through the regulation of major cell signaling pathways, such as AKT and NF-κB. Therefore, those are the subject of intense study. There are a number of marine derived compounds related to research topic progress worldwide. Here is a list of some marine derived bioactive compounds that show evidence that they regulate cell survival and apoptosis mechanisms (Table 1).
PI3K/AKT pathway transmits antiapoptotic survival signals, and their inhibition by marine compounds could be a potential therapeutic value. Agents capable of suppressing AKT and/or NF-κB activation have therapeutic promise and the potential to inhibit carcinogenesis. The pathway-based phosphor profiling approach identifies and quantifies clinically relevant, drug-specific biomarkers for PI3K pathway inhibitors that target AKT, phosphoinositide dependent kinase 1 (PDK1), PI3K and mammalian target of rapamycin (mTOR) . Arenamides (A-C) from the fermentation broth of a marine bacterial strain Salinispora arenicola blocked TNF-induced activation of NF-κB in a dose- and time-dependent manner . Even marine derived sediments are rich in bioactive substances, which may interact with NF-κB, thereby inducing apoptosis in cancer cells.
Heteronemin, a marine sesterterpene isolated from the sponge Hyrtios sp., inhibits NF-κB activation and activates both initiator caspases -8 and -9, which are implicated in the extrinsic and intrinsic apoptotic pathway, respectively, in chronic myelogenous leukemia cells . Tyrindoleninone and 6-bromoisatin are indole derivatives from marine mollusc Dicathais orbita and induce apoptosis in female reproductive cancer cell lines for ovary, granulosa and choriocarcinoma (OVCAR-3, KGN and Jar), respectively. Further studies, including investigation of initiator caspase 8 and 9, could help discriminate between the extrinsic and intrinsic pathway for the induction of apoptosis by these compounds . The ester-substituted sesquiterpenoid cryptosphaerolide is isolated from the marine-derived ascomycete fungal strain CNL-523 (Cryptosphaeria sp.). Cryptosphaerolide was found to be an inhibitor of the protein Mcl-1(induced myeloid leukemia cell differentiation protein), a cancer drug target involved in apoptosis in HCT-116 human colon carcinoma cell line, indicating that the compound may be of value in exploring the Mcl-1 as a target for cancer chemotherapy . Mcl-1, an anti-apoptotic member of the Bcl-2 family, sequesters Bak on the outer mitochondrial membrane, thereby preventing Bak oligomerization. Inhibition of Mcl-1promote cytochrome release, thereby, induces apoptosis.
Makaluvamine A is a pyrroloquinoline, principally isolated from the sponge Zyzzya fuliginosa, and is known to have potent anticancer activity in HCT-116 cells . Ascididemin (ASC), an aromatic alkaloid isolated from the Mediterranean ascidian Cystodytes dellechiajei , is a strong inducer of apoptosis in HL-60 and P388 leukemia cells . Another alkaloid, Lamellarin D (LAM-D), initially isolated from a prosobranch mollusc of the genus Lamellaria, exhibits cytotoxicity against many different tumors. LAM-D potently stabilizes topoisomerase I DNA covalent complexes to promote the formation of DNA single strand breaks. LAM-D also promotes nuclear apoptosis in leukemia cells via the intrinsic apoptotic pathway. Activation of Bax decreases the expression of antiapoptotic proteins Bcl-2 in association with activation of caspase-9 and caspase-3 [114,115]. Spongistatin 1, a macrocyclic lactone isolated from the marine sponges Spirastrella spinispirulifera and Hyrtios erecta induces apoptosis by interacting with the caspase-dependent pathway by the release of cytochrome c, Smac/DIABLO and Omi/HtrA2 from the mitochondria to the cytosol, leading to apoptosis in Jurkat cells . Streptochlorin is a small molecule isolated from Streptomyces sp. and exhibits selective cytotoxicity against several cancer cell lines [117,118]. Streptochlorin induces apoptosis in human leukemic U937 cancer cells by a dose- and time-dependent manner by modulation of the Fas/Fas ligand (FasL) system, downregulating anti-apoptotic Bcl-2 expression and upregulating pro-apoptotic protein Bax .
Seaweeds belong to a group of plants known as algae. Seaweeds are classified as Rhodophyta (red algae), Phaeophyta (brown algae) or Chlorophyta (green algae), depending on their nutrient, pigment and chemical composition. Like other plants, seaweed contains various inorganic and organic substances, which could benefit human health . Seaweed has a variety of chemoprotective compounds, such as flavonoids and other phenolic compounds . The brown algae Ecklonia Cava (Laminariaceae), distributed abundantly in the sea all over the world, has been used as a seasonal vegetable in coastal areas. This is one of the seaweeds growing at a water depth of 2–25 m in the sublittoral zone along the coast of Korea . Phloroglucinol, derived from Ecklonia Cava, causes cancer inhibition in MCF-7 human breast cancer cells and induces apoptosis . Marine bioactive compounds interactions on the signaling molecules are depicted in the Figure 4.
Marine derived polysaccharides from microorganisms and seaweed have potential effects in the biomedical and pharmaceutical fields. Chitosan is produced commercially by deacetylation of chitin, which has the structural element in the exoskeleton of crustaceans (such as crabs and shrimp) and cell walls of fungi. Chitosan, a copolymer consisting of (1→4)-2-acetamido-d-glucose and (1→4)-2-amino-d-glucose units, is derived from chitin by deacetylation. Chitosan is the second most abundant polysaccharide in nature, and its production is low cost and ecologically appealing. Due to its biocompatibility and less toxic nature, it is being developed as a new physiologically bioactive material, because it possesses various biological activities, such as antioxidant and antitumor activity . Diethylaminoethyl chitosan induces apoptosis in HeLa cells via activation of caspase-3 and p53 expression . Fucoidan is the collective name for algal sulfated polysaccharides extracted from the brown seaweeds, and the structure of fucoidan consists mainly of polymers formed by branched polysaccharide sulfate esters with a l-fucose building block. Fucoidan has shown cytotoxic effects and induces apoptosis in MCF-7 cells. This apoptotic effect is triggered via the mitochondrial mediated pathway accompanied by activation of caspase-9 .
Apoptosis is mainly regulated by cell survival and the proliferative signal transduction pathway that is critically involved in human cancers. A balance of cell proliferation, survival and apoptosis normally maintains cellular homeostasis. However, apoptosis is a very complex process with numerous specific targets within each arm of the apoptotic pathways. Targeting cell survival and apoptosis signaling by marine compounds are useful for cancer therapeutics. Future research is required to identify the marine bioactive compounds-specific molecular mechanism for chemopreventive agents. The potential benefits of cancer chemoprevention from clinical trials and pre-clinical studies could be useful for cancer therapy.
This study was supported by a grant from Marine Bioprocess Research Center of the Marine Bio 21 Project funded by the Ministry of Land, Transport and Maritime, Republic of Korea.
Conflict of Interest
The authors declare no conflict of interest.
- Lockshin, R.A.; Zakeri, Z. Cell death in health and disease. J. Cell. Mol. Med 2007, 11, 1214–1224. [Google Scholar]
- Duprez, L.; Wirawan, E.; Vanden Berghe, T.; Vandenabeele, P. Major cell death pathways at a glance. Microbes Infect 2009, 11, 1050–1062. [Google Scholar]
- Sprick, M.R.; Walczak, H. The interplay between the Bcl-2 family and death receptor-mediated apoptosis. Biochim. Biophys. Acta 2004, 1644, 125–132. [Google Scholar]
- Whelan, R.S.; Kaplinskiy, V.; Kitsis, R.N. Cell death in the pathogenesis of heart disease: Mechanisms and significance. Annu. Rev. Physiol 2010, 72, 19–44. [Google Scholar]
- Engelman, J.A. Targeting PI3K signaling in cancer: Opportunities, challenges and limitations. Nat. Rev. Cancer 2009, 9, 550–562. [Google Scholar]
- Fulda, S.; Galluzzi, L.; Kroemer, G. Targeting mitochondria for cancer therapy. Nat. Rev. Drug Discov 2010, 9, 447–464. [Google Scholar]
- Parcellier, A.; Tintignac, L.A.; Zhuravleva, E.; Hemmings, B.A. PKB and the mitochondria: AKT signaling on apoptosis. Cell. Signal 2008, 20, 21–30. [Google Scholar]
- Shaw, R.J.; Cantley, L.C. Ras, PI(3)K and mTOR signalling controls tumour cell growth. Nature 2006, 441, 424–430. [Google Scholar]
- Hsieh, A.C.; Truitt, M.L.; Ruggero, D. Oncogenic AKT activation of translation as a therapeutic target. Br. J. Cancer 2011, 105, 329–336. [Google Scholar]
- Yin, Y.; Shen, W.H. PTEN: A new guardian of the genome. Oncogene 2008, 27, 5443–5453. [Google Scholar]
- Yamaguchi, H.; Wang, H.G. The protein kinase PKB/Akt regulates cell survival and apoptosis by inhibiting Baxc onformational change. Oncogene 2001, 20, 7779–7786. [Google Scholar]
- Gardai, S.J.; Hildeman, D.A.; Frankel, S.K.; Whitlock, B.B.; Frasch, S.C.; Borregaard, N.; Marrack, P.; Bratton, D.L.; Henson, P.M. Phosphorylation of Bax Ser184 by Akt regulates its activity and apoptosis in neutrophils. J. Biol. Chem 2004, 279, 21085–21095. [Google Scholar]
- Qi, X.J.; Wildey, G.M.; Howe, P.H. Evidence that Ser87 of Bim EL is phosphorylated by Akt and regulates BimEL apoptotic function. J. Biol. Chem 2006, 281, 813–823. [Google Scholar]
- Dan, H.C.; Sun, M.; Kaneko, S.; Feldman, R.I.; Nicosia, S.V.; Wang, H.G.; Tsang, B.K.; Cheng, J.Q. Akt phosphorylation and stabilization of X-linked inhibitor of apoptosis protein (XIAP). J. Biol. Chem 2004, 279, 5405–5412. [Google Scholar]
- Maurer, U.; Charvet, C.; Wagman, A.S.; Dejardin, E.; Green, D.R. Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of MCL-1. Mol. Cell 2006, 21, 749–760. [Google Scholar]
- Van Der Heide, L.P.; Hoekman, M.F.M.; Smidt, M.P. The ins and outs of FoxO shuttling: Mechanisms of FoxO translocation and transcriptional regulation. Biochem. J 2004, 380, 297–309. [Google Scholar]
- De Keizer, P.L.; Burgering, B.M.; Dansen, T.B. Forkhead boxo as a sensor, mediator and regulator of redox signaling. Antioxid. Redox Signal 2011, 14, 1093–1106. [Google Scholar]
- Ozes, O.N.; Mayo, L.D.; Gustin, J.A.; Pfeffer, S.R.; Pfeffer, L.M.; Donner, D.B. NF-kappa B activation by tumour necrosis factor requires the Akt serine-threonine kinase. Nature 1999, 401, 82–85. [Google Scholar]
- Romashkova, J.A.; Makarov, S.S. NF-kappaB is a target of AKT in anti-apoptotic PDGF signalling. Nature 1999, 401, 86–90. [Google Scholar]
- Bender, A.; Opel, D.; Naumann, I.; Kappler, R.; Friedman, L.; von Schweinitz, D.; Debatin, K.M.; Fulda, S. PI3K inhibitors prime neuroblastoma cells for chemotherapy by shifting the balance towards pro-apoptotic Bcl-2proteins and enhanced mitochondrial apoptosis. Oncogene 2011, 30, 494–503. [Google Scholar]
- Opel, D.; Naumann, I.; Schneider, M.; Bertele, D.; Debatin, K.M.; Fulda, S. Targeting aberrant PI3K/Akt activation by PI 103 restores sensitivity to TRAIL-induced apoptosis in neuroblastoma. Clin. Cancer Res 2011, 17, 3233–3247. [Google Scholar]
- Obexer, P.; Geiger, K.; Ambros, P.F.; Meister, B.; Ausserlechner, M.J. FKHRL1-mediated expression of Noxa and Bim induces apoptosis via the mitochondria in neuroblastoma cells. Cell Death Differ 2007, 14, 534–547. [Google Scholar]
- Ploner, C.; Kofler, R.; Villunger, A. Noxa: At the tip of the balance between life and death. Oncogene 2008, 27, 84–92. [Google Scholar]
- Johnstone, R.W.; Ruefli, A.A.; Lowe, S.W. Apoptosis, a link between cancer genetics and chemotherapy. Cell 2002, 108, 153–164. [Google Scholar]
- Fulda, S.; Debatin, K.M. Extrinsic versus intrinsic apoptosis pathways in anti cancer chemotherapy. Oncogene 2006, 25, 4798–4811. [Google Scholar]
- Fulda, S.; Vucic, D. Targeting IAP proteins for therapeutic intervention in cancer. Nat. Rev. Drug Discov 2012, 11, 109–124. [Google Scholar]
- Levine, A. P53, the cellular gatekeeper for growth and division. Cell. Mol. Biol 1997, 88, 323–331. [Google Scholar]
- Yin, C.; Knudson, C.; Korsmeyer, S.; Dyke, T.V. Bax suppresses tumorigenesis and stimulates apoptosis in vivo. Nature 1997, 385, 637–640. [Google Scholar]
- Pietenpol, J.; Stewart, Z. Cell cycle checkpoint signaling, cell cycle arrest versus apoptosis. Toxicology 2002, 475, 181–182. [Google Scholar]
- Johnson, T.; Yu, Z.; Ferrans, V.; Lowenstein, R.; Finkel, T. Reactive oxygen species are downstream mediators of p53-dependent apoptosis. Proc. Natl. Acad. Sci. USA 1996, 93, 11848–11852. [Google Scholar]
- Sheikh, M.; Fornace, A.J. Role of p53 family members in apoptosis. J. Cell. Physiol 2000, 182, 171–181. [Google Scholar]
- Martindale, J.; Holbrook, N. Cellular response to oxidative stress, signaling for suicide and survival. J. Cell. Physiol 2002, 192, 1–15. [Google Scholar]
- Saelens, X.; Festjens, N.; Walle, L.V.; van Gurp, M.; van Loo, G.; Vandenabeele, P. Toxic proteins released from mitochondria in cell death. Oncogene 2004, 23, 2861–2874. [Google Scholar]
- Du, C.; Fang, M.; Li, Y.; Li, L.; Wang, X. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell 2000, 102, 33–42. [Google Scholar]
- Van Loo, G.; van Gurp, M.; Depuydt, B.; Srinivasula, S.M.; Rodriguez, I.; Alnemri, E.S.; Gevaert, K.; Vandekerckhove, J.; Declercq, W.; Vandenabeele, P. The serine protease Omi/HtrA2 is released from mitochondria during apoptosis. Omi interacts with caspase-inhibitor XIAP and induces enhanced caspase activity. Cell Death Differ 2002, 9, 20–26. [Google Scholar]
- Garrido, C.; Galluzzi, L.; Brunet, M.; Puig, P.E.; Didelot, C.; Kroemer, G. Mechanisms of cytochrome c release from mitochondria. Cell Death Differ 2006, 13, 1423–1433. [Google Scholar]
- Chinnaiyan, A.M. The apoptosome, heart and soul of the cell death machine. Neoplasia 1999, 1, 5–15. [Google Scholar]
- Hill, M.M.; Adrain, C.; Duriez, P.J.; Creagh, E.M.; Martin, S.J. Analysis of the composition, assembly kinetics and activity of native Apaf-1 apoptosomes. EMBO J 2002, 23, 2134–2145. [Google Scholar]
- Schimmer, A.D. Inhibitor of apoptosis proteins, translating basic knowledge into clinical practice. Cancer Res 2004, 64, 7183–7190. [Google Scholar]
- Ekert, P.G.; Vaux, D.L. The mitochondrial death squad, hardened killers or innocent bystanders? Curr. Opin. Cell Biol 2005, 17, 626–630. [Google Scholar]
- Joza, N.; Susin, S.A.; Daugas, E.; Stanford, W.L.; Cho, S.K.; Li, C.Y.; Sasaki, T.; Elia, A.J.; Cheng, H.Y.M.; Ravagnan, L.; et al. Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death. Nature 2001, 410, 549–554. [Google Scholar]
- Susin, S.A.; Daugas, E.; Ravagnan, L.; Samejima, K.; Zamzami, N.; Loeffler, M.; Costantini, P.; Ferri, K.F.; Irinopoulou, T.; Prévost, M.-C.; et al. Two distinct pathways leading to nuclear apoptosis. J. Exp. Med 2000, 192, 571–580. [Google Scholar]
- Li, L.Y.; Luo, X.; Wang, X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature 2001, 412, 95–99. [Google Scholar]
- Enari, M.; Sakahira, H.; Yokoyama, H.; Okawa, K.; Iwamatsu, A.; Nagata, S. A caspase-activated DNase that degrades DNA during apoptosis and its inhibitor ICAD. Nature 1998, 391, 43–50. [Google Scholar]
- Cory, S.; Adams, J.M. The Bcl2 family, regulators of the cellular life-or-death switch. Nat. Rev. Cancer 2002, 2, 647–656. [Google Scholar]
- Schuler, M.; Green, D.R. Mechanisms of p53-dependent apoptosis. Biochem. Soc. Trans 2001, 29, 684–688. [Google Scholar]
- Vaux, D.L.; Cory, S.; Adamsm, J.M. Bcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells. Nature 1988, 335, 440–442. [Google Scholar]
- Weyhenmeyer, B.; Murphy, A.C.; Prehn, J.H.; Murphy, B.M. Targeting the anti-apoptotic bcl-2 family members for the treatment of cancer. Exp. Oncol 2012, 34, 192–199. [Google Scholar]
- Tsujimoto, Y.; Finger, L.R.; Yunis, J.; Nowell, P.C.; Croce, C.M. Cloning of the chromosome breakpoint of neoplastic B cells with the t (14;18) chromosome translocation. Science 1984, 226, 1097–1099. [Google Scholar]
- Reed, J.C. Bcl-2 family proteins, regulators of apoptosis and chemoresistance in hematologic malignancies. Semin. Hematol 1997, 34, 9–19. [Google Scholar]
- Portt, L.; Norman, G.; Clapp, C.; Greenwood, M.; Greenwood, T.M. Anti-apoptosis and cell survival: A review. Biochim. Biophys. Acta 2011, 1813, 238–259. [Google Scholar]
- Reed, J.C. Bcl-2 and the regulation of programmed cell death. J. Cell. Biol 1994, 124, 1–6. [Google Scholar]
- Yip, K.W.; Reed, J.C. Bcl-2 family proteins amd cancer. Oncogene 2008, 27, 6398–6406. [Google Scholar]
- Scorrano, L.; Korsmeyer, S. Mechanisms of cytochrome c release by proapoptotic BCL-2 family members. Biochem. Biophys. Res. Commun 2003, 304, 437–444. [Google Scholar]
- Korsmeyer, S.J. Regulators of cell death. Trends Genet 1995, 11, 101–105. [Google Scholar]
- Lee, H.J.; Lee, H.J.; Lee, E.O.; Ko, S.G.; Bae, H.S.; Kim, C.H.; Ahn, K.S.; Lu, J.; Kim, S.H. Mitochondria-cytochrome C-caspase-9 cascade mediates isorhamnetin-induced apoptosis. Cancer Lett 2008, 207, 342–353. [Google Scholar]
- Fiandalo, M.V.; Kyprianou, N. Caspase control: Protagonists of cancer cell apoptosis. Exp. Oncol 2012, 34, 165–175. [Google Scholar]
- Reed, J. Apoptosis-targeted therapies for cancer. Cancer Cell 2003, 3, 9–17. [Google Scholar]
- Zapata, J.M.; Pawlowski, K.; Haas, E.; Ware, C.F.; Godzik, A.; Reed, J.C. A diverse family of proteins containing tumor necrosis factor receptor-associated factor domains. J. Biol. Chem 2001, 276, 24242–24252. [Google Scholar]
- Lamy, T.; Liu, J.H.; Landowski, T.H.; Dalton, W.S.; Loughran, T.P., Jr. Dysregulation of CD95/CD95 ligand-apoptotic pathway in CD3 large granular lymphocyte leukemia. Blood 1998, 92, 4771–4777. [Google Scholar]
- Landowski, T.H.; Moscinski, L.; Burke, R.; Buyuksal, I.; Painter, J.S.; Goldstein, S.; Dalton, W.S. CD95 antigen mutations in hematopoietic malignancies. Leuk. Lymphoma 2001, 42, 835–846. [Google Scholar]
- Krammer, P.H. CD95’s deadly mission in the immune system. Nature 2000, 407, 789–795. [Google Scholar]
- Wajant, H. The Fas signaling pathway, more than a paradigm. Science 2002, 296, 1635–1636. [Google Scholar]
- Landowski, T.H.; Qu, N.; Buyuksal, I.; Painter, J.S.; Dalton, W.S. Mutations in the Fas antigen in patients with multiple myeloma. Blood 1997, 90, 4266–4270. [Google Scholar]
- Irie, S.; Li, Y.; Kanki, H.; Ohyama, T.; Deaven, L.L.; Somlo, S.; Sato, T.A. Identification of two Fas-associated phosphatase-1 (FAP-1) promoters in human cancer cells. DNA Seq 2001, 11, 519–526. [Google Scholar]
- Krueger, A.; Baumann, S.; Krammer, P.H.; Kirchhoff, S. FLICE-inhibitory proteins, regulators of death receptor-mediated apoptosis. Mol. Cell. Biol 2001, 21, 8247–8254. [Google Scholar]
- Strasser, A.; O’Connor, L.; Dixit, V.M. Apoptosis signaling. Annu. Rev. Biochem 2000, 69, 217–245. [Google Scholar]
- Henson, P.M.; Bratton, D.L.; Fadok, V.A. Apoptotic cell removal. Curr. Biol 2001, 11, 795–805. [Google Scholar]
- Green, D.R.; Evan, G.I. A matter of life and death. Cancer Cell 2002, 1, 19–30. [Google Scholar]
- Thornberry, N.A.; Lazebnik, Y. Caspases, enemies within. Science 1998, 281, 1312–1316. [Google Scholar]
- Mancini, M.; Nicholson, D.W.; Roy, S.; Thornberry, N.A.; Peterson, E.P.; Casciola-Rosen, L.A.; Rosen, A. The caspase-3 precursor has a cytosolic and mitochondrial distribution, implications for apoptotic signaling. J. Cell Biol 1998, 140, 1485–1495. [Google Scholar]
- Hengartner, M.O. The biochemistry of apoptosis. Nature 2000, 407, 770–776. [Google Scholar]
- Cohen, G.M. Caspases, the executioners of apoptosis. Biochem. J 1997, 336, 1–16. [Google Scholar]
- Rai, N.K.; Tripathi, K.; Sharma, D.; Shukla, V.K. Apoptosis, a basic physiologic process in wound healing. Int. J. Low Extrem. Wounds 2005, 4, 138–144. [Google Scholar]
- Hu, S.; Snipas, S.J.; Vincenz, C.; Salvesen, G.; Dixit, V.M. Caspase-14 is a novel developmentally regulated protease. J. Biol. Chem 1998, 273, 29648–29653. [Google Scholar]
- Nakagawa, T.; Zhu, H.; Morishima, N.; Li, E.; Xu, J.; Yankner, B.A.; Yuan, J. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta. Nature 2000, 403, 98–103. [Google Scholar]
- Koenig, U.; Eckhart, L.; Tschachler, E. Evidence that caspase-13 is not a human but a bovine gene. Biochem. Biophys. Res. Commun 2001, 285, 1150–1154. [Google Scholar]
- Kang, S.J.; Wang, S.; Kuida, K.; Yuan, J. Distinct downstream pathways of caspase-11 in regulating apoptosis and cytokine maturation during septic shock response. Cell Death Differ 2002, 9, 1115–1125. [Google Scholar]
- Nemes, Z., Jr; Friis, R.R.; Aeschlimann, D.; Saurer, S.; Paulsson, M.; Fésüs, L. Expression and activation of tissue transglutaminase in apoptotic cells of involuting rodent mammary tissue. Eur. J. Cell Biol. 1996, 70, 125–133. [Google Scholar]
- Bortner, C.D.; Oldenburg, N.B.; Cidlowski, J.A. The role of DNA fragmentation in apoptosis. Trends Cell Biol 1995, 5, 21–26. [Google Scholar]
- Bratton, D.L.; Fadok, V.A.; Richter, D.A.; Kailey, J.M.; Guthrie, L.A.; Henson, P.M. Appearance of phosphatidylserine on apoptotic cells requires calcium-mediated nonspecific flip-flop and is enhanced by loss of the aminophospholipid translocase. J. Biol. Chem 1997, 272, 26159–26165. [Google Scholar]
- Arur, S.; Uche, U.E.; Rezaul, K.; Fong, M.; Scranton, V.; Cowan, A.E.; Mohler, W.; Han, D.K. Annexin I is an endogenous ligand that mediates apoptotic cell engulfment. Dev. Cell 2003, 4, 587–598. [Google Scholar]
- Gardai, S.J.; McPhillips, K.A.; Frasch, S.C.; Janssen, W.J.; Starefeldt, A.; Murphy-Ullrich, J.E.; Bratton, D.L.; Oldenborg, P.-A.; Michalak, M.; Henson, P.M. Cell-surface calreticulin initiates clearance of viable or apoptotic cells through trans-activation of LRP on the phagocyte. Cell 2005, 123, 321–334. [Google Scholar]
- Kuno, T.; Tsukamoto, T.; Hara, A.; Tanaka, T. Cancer chemoprevention through the induction of apoptosis by natural compounds. J. Biophys. Chem 2012, 3, 156–173. [Google Scholar]
- Fisher, B.; Perera, F.; Cooke, A.; Opeitum, A; Stitt, L. Long-term follow-up of axillary node-positive breast cancer patients receiving adjuvant tamoxifen alone, patterns of recurrence. Int. J. Radiat. Oncol. Biol. Phys. 1998, 42, 117–123. [Google Scholar]
- Cummings, S.; Eckert, S.; Krueger, K.; Grady, D.; Powles, T.J.; Cauley, J.A.; Norton, L.; Nickelsen, T.; Bjarnason, N.H.; Morrow, M.; et al. The effect of raloxifene on risk of breast cancer in postmenopausal women, results from the MORE randomized trial. Multiple Outcomes of Raloxifene Evaluation. J. Am. Med. Assoc 1999, 281, 2189–2197. [Google Scholar]
- Veronesi, U.; de Palo, G.; Marubini, E.; Costa, A.; Formelli, F.; Mariani, L.; Decensi, A.; Camerini, T.; del Turco, M.R.; di Mauro, M.G.; et al. Randomized trial of fenretinide to prevent second breast malignancy in women with early breast cancer. J. Natl. Cancer Inst 1999, 91, 1847–1856. [Google Scholar]
- Hail, N., Jr; Cortes, M.; Drake, E.N.; Spallholz, J.E. Cancer chemoprevention: A radical perspective. Free Rad. Biol. Med. 2008, 45, 97–110. [Google Scholar]
- Kelloff, G.J.; Sigman, C.C.; Greenwald, P. Cancer chemoprevention: Progress and promise. Eur. J. Cancer 1999, 35, 2031–2038. [Google Scholar]
- Kakizoe, T. Chemoprevention of cancer-Focusing on clinical trials. Jpn. J. Clin. Oncol 2003, 33, 421–442. [Google Scholar]
- Park, E.J.; Pezzuto, J.M. Botanicals in cancer chemoprevention. Cancer Metast. Rev 2002, 21, 231–255. [Google Scholar]
- Stan, S.D.; Kar, S.; Stoner, G.D.; Singh, S.V. Bioactive food components and cancer risk reduction. J. Cell. Biochem 2008, 104, 339–356. [Google Scholar]
- Newman, D.J.; Cragg, G.M. Natural products as sources of new drugs over the last 25 years. J. Nat. Prod 2007, 70, 461–477. [Google Scholar]
- Blunt, J.W.; Copp, B.R.; Munro, M.H.G.; Northcote, P.T.; Prinsep, M.R. Marine natural products. Nat. Prod. Rep 2011, 28, 196–268. [Google Scholar]
- Dumontet, C.; Jordan, M.A. Microtubule-binding agents: A dynamic field of cancer therapeutics. Nat. Rev. Drug. Discov 2010, 17, 790–803. [Google Scholar]
- Gullett, N.P.; Ruhul Amin, A.R.; Bayraktar, S.; Pezzuto, J.M.; Shin, D.M.; Khuri, F.R.; Aggarwal, B.B.; Surh, Y.J.; Kucuk, O. Cancer prevention with natural compounds. Semin. Oncol 2010, 37, 258–281. [Google Scholar]
- Hickford, S.J.; Blunt, J.W.; Munro, M.H. Antitumour polyether macrolides, four new halichondrins from the New Zealand deep-water marine sponge Lissodendoryx sp. Bioorg. Med. Chem 2009, 17, 2199–2203. [Google Scholar]
- Aoki, S.; Cao, L.; Matsui, K.; Rachmat, R.; Akiyamac, S.; Kobayashia, M. Kendarimide A, a novel peptide reversing P-glycoprotein-mediated multidrug resistance in tumor cells, from a marine sponge of Haliclona sp. Tetrahedron 2004, 60, 7053–7059. [Google Scholar]
- Thomas, T.R.A.; Kavlekar, D.P.; LokaBharathi, P.A. Marine Drugs from Sponge-Microbe Association—A Review. Mar. Drugs 2010, 8, 1417–1468. [Google Scholar]
- Rodriguez, A.D.; Martinez, N. Marine antitumor agents, 14-deoxycrassin and pseudoplexaurol, new cembranoid diterpenes from the Caribbean gorgonian Pseudoplexaura porosa. Experientia 1993, 49, 179–181. [Google Scholar]
- Olano, C.; Méndez, C.; Salas, J.A. Antitumor Compounds from Marine Actinomycetes. Mar. Drugs 2009, 7, 210–248. [Google Scholar]
- Hassan, H.M.; Khanfar, M.A.; Elnagar, A.Y.; Mohammed, R.; Shaala, L.A.; Youssef, D.T.A.; Hifnawy, M.S.; Sayed, K.A.E. Pachycladins A–E, prostate cancer invasion and migration inhibitory Eunicellin-based diterpenoids from the red sea soft coral Cladiella pachyclados. J. Nat. Prod 2010, 73, 848–853. [Google Scholar]
- Luqman, S.; Pezzuto, J.M. NFkappa B: A promising target for natural products in cancer chemoprevention. Phytother. Res 2010, 24, 949–963. [Google Scholar]
- Karin, M. Nuclear factor-kappa B in cancer development and progression. Nature 2006, 441, 431–436. [Google Scholar]
- Crowell, J.A.; Steele, V.E.; Fay, J.R. Targeting the AKT protein kinase for cancer chemoprevention. Mol. Cancer Therap 2007, 6, 2139–2148. [Google Scholar]
- Andersen, J.N.; Sathyanarayanan, S.; di Bacco, A.; Chi, A.; Zhang, T.; Chen, A.H.; Dolinski, B.; Kraus, M.; Roberts, B.; Arthur, W.; et al. Pathway-based identification of biomarkers for targeted therapeutics, Personalized oncology with PI3K pathway inhibitors. Sci. Transl. Med 2010, 2, 43–55. [Google Scholar]
- Asolkar, R.N.; Freel, K.C.; Jensen, P.R.; Fenical, W.; Kondratyuk, T.P.; Park, E.J.; Pezzuto, J.M. Arenamides A–C, cytotoxic NF kappa B inhibitors from the marine actinomycetes Salinispora arenicola. J. Nat. Prod 2009, 72, 396–402. [Google Scholar]
- Schumacher, M.; Cerella, C.; Eifes, S.; Chateauvieux, S.; Morceau, F.; Jaspars, M.; Dicato, M.; Diederich, M. Heteronemin, a spongean sesterterpene, inhibits TNF alpha-induced NF-kappa B activation through proteasome inhibition and induces apoptotic cell death. Biochem. Pharmacol 2010, 79, 610–622. [Google Scholar]
- Edwards, V.; Benkendorff, K.; Young, F. Marine Compounds Selectively Induce Apoptosis in Female Reproductive Cancer Cells but Not in Primary-Derived Human Reproductive Granulosa Cells. Mar. Drugs 2012, 10, 64–83. [Google Scholar]
- Oh, H.; Jensen, P.R.; Murphy, B.T.; Fiorilla, C.; Sullivan, J.F.; Ramsey, T.; Fenical, W. Cryptosphaerolide, a cytotoxic Mcl-1 inhibitor from a marine-derived ascomycete related to the genus Cryptosphaeria. J. Nat. Prod 2010, 73, 998–1001. [Google Scholar]
- Dias, N.; Vezin, H.; Lansiaux, A.; Bailly, C. Topoisomerase Inhibitors of Marine Origin and Their Potential Use as Anticancer Agents. Top. Curr. Chem 2005, 253, 89–108. [Google Scholar]
- Bonnard, I.; Bontemps, N.; Lahmy, S.; Banaigs, B.; Combaut, G.; Francisco, C.; Colson, P.; Houssier, C.; Waring, M.J.; Bailly, C. Binding to DNA and cytotoxic evaluation of ascididemin, the major alkaloid from the Mediterranean ascidian Cystodytes dellechiajei. Anticancer Drug Des 1995, 10, 333–346. [Google Scholar]
- Dassonneville, L.; Wattez, N.; Baldeyrou, B.; Mahieu, C.; Lansiaux, A.; Banaigs, B.; Bonnard, I.; Bailly, C. Inhibition of topoisomerase II by the marine alkaloid ascididemin and induction of apoptosis in leukemia cells. Biochem. Pharmacol 2000, 60, 527–537. [Google Scholar]
- Tardy, C.; Facompré, M.; Laine, W.; Baldeyroua, B.; arc a-Gravalosb, D.; Franceschb, A.; Mateob, C.; Pastorb, A.; Jiménezb, J.A.; Manzanares, I.; et al. Topoisomerase I-mediated DNA cleavage as a guide to the development of antitumor agents derived from the marine alkaloid lamellarin D, triester derivatives incorporating amino acid residues. Bioorg. Med. Chem 2004, 12, 1697–1712. [Google Scholar]
- Ballot, C.; Kluza, J.; Martoriati, A.; Nyman, U.; Formstecher, P.; Joseph, B.; Bailly, C.; Marchetti, P. Essential role of mitochondria in apoptosis of cancer cells induced by the marine alkaloid Lamellarin D. Mol. Cancer Ther 2009, 8, 3307–3317. [Google Scholar]
- Schyschka, L.; Rudy, A.; Jeremias, I.; Barth, N.; Pettit, G.R.; Vollmar, A.M. Spongistatin 1: A new chemosensitizingmarine compoundthat degrades XIAP. Leukemia 2008, 22, 1737–1745. [Google Scholar]
- Shin, H.J.; Jeong, H.S.; Lee, H.S.; Park, S.K.; Hwan, M.K.; Ho, J.K. Isolation and structure determination of streptochlorin, an antiproliferative agent from a marine-derived Streptomyces sp. 04DH110. J. Microbiol. Biotech 2007, 17, 1403–1406. [Google Scholar]
- Choi, I.K.; Shin, H.J.; Lee, H.S.; Kwon, H.J. Streptochlorin, a marine natural product, inhibits NF-κB activation and suppresses angiogenesis in vitro. J. Microbiol. Biotech 2007, 17, 1338–1343. [Google Scholar]
- Park, C.; Shin, H.J.; Kim, G.Y.; Kwon, T.K.; Nam, T.J.; Kim, S.K.; Cheong, J.; Choi, I.W.; Choi, Y.H. Induction of apoptosis by streptochlorin isolated from Streptomyces sp. in human leukemic U937 cells. Toxicol. In Vitro 2008, 22, 1573–1581. [Google Scholar]
- Ali, M.S.; Jahangir, M.; Saleem, M.; Pervez, M.K.; Hameed, S.; Ahmad, V.U. Metabolites of marine algae collected from Karachi-coasts of Arabian sea. Nat. Prod. Sci 2000, 6, 61–65. [Google Scholar]
- Rice-Evans, C.A.; Miller, N.J.; Paganga, G. Antioxidant properties of phenolic compounds. Trends Plant Sci 1997, 2, 152–159. [Google Scholar]
- Maegawa, M.; Yokohama, Y.; Aruga, Y. Critical light conditions for young Ecklonia cava and Eisenia bicyclis with reference to photosynthesis. Hydrobiologia 1987, 151, 447–455. [Google Scholar]
- Kong, S.C.; Kim, A.J.; Yoon, Y.N.; Kim, S.K. Induction of apoptosis by phloroglucinol derivative from Ecklonia Cava in MCF-7 human breast cancer cells. Food Chem. Toxicol 2009, 47, 1653–1658. [Google Scholar]
- Qin, C.; Du, Y.; Xiao, L.; Li, Z.; Gao, X. Enzymic preparation of water-soluble chitosan and their antitumor activity. Int. J. Biol. Macromol 2002, 31, 111–117. [Google Scholar]
- Lee, H.S.; Ryua, B.; Je, Y.J.; Kim, S.K. Diethylaminoethyl chitosan induces apoptosis in HeLa cells via activation of caspase-3 and p53 expression. Carbohydr. Polym 2011, 84, 571–578. [Google Scholar]
- Yamasaki-Miyamoto, Y.; Yamasaki, M.; Tachibana, H.; Yamada, K. Fucoidan Induces Apoptosis through Activation of Caspase-8 on Human Breast Cancer MCF-7 Cells. J. Agric. Food Chem 2009, 57, 8677–8682. [Google Scholar]
|No||Name of the Compound||Source of Organisms||Chemical class||Cancer Target||Reference|
|1||Arenamides A–C||Actinomycete (Salinispora arenicola)||Cyclohexa-depsipeptides||Human colon carcinoma cell line (HCT-116)|||
|2||Heteronemin||Sponge (Hyrtios sp.)||Sesterterpene||Leukemia (K562 cells)|||
|3||6-bromoisatin||Whelk (Dicathais orbita)||Indole derivative||Ovary, granulosa, Choriocarcinoma (OVCAR-3, KGN, Jar)|||
|4||Tyrindoleninone||Whelk (Dicathais orbita)||Indole derivative||Ovary, granulosa, Choriocarcinoma (OVCAR-3, KGN, Jar)|||
|5||Cryptosphaerolide||Ascomycete fungal strain CNL-523 (Cryptosphaeria sp.)||sesquiterpenoid||Human colon carcinoma cell line (HCT-116)|||
|6||Makaluvamine A||sponge (Zyzzya fuliginosa)||pyrroloquinoline||Colon cancer (HCT-116 cells)|||
|7||Ascididemin||Actinomycete (Salinispora arenicola)||Cyclohexa-depsipeptides||Human colon carcinoma cell line (HCT-116)||[112,113]|
|8||Lamellarin D||Prosobranch mollusc of the genus (Lamellaria)||Alkaloid||Leukemia||[114,115]|
|9||Spongistatin 1||Sponges (Spirastrella spinispirulifera and Hyrtios erecta)||macrocyclic lactone||Leukemia (Jurkat cells)|||
|10||Streptochlorin||Streptomyces sp.||Methyl pyridine||Leukemia (U937 cells)||[117–119]|
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