Int. J. Mol. Sci. 2013, 14(12), 24211-24229; doi:10.3390/ijms141224211
Article

Molecular Cloning and Expression of CYP9A61: A Chlorpyrifos-Ethyl and Lambda-Cyhalothrin-Inducible Cytochrome P450 cDNA from Cydia pomonella

1 Key Laboratory of Plant Protection Resources & Pest Management of Ministry of Education, Northwest A&F University, Yangling 712100, Shaanxi, China 2 Department of Entomology, University of Arizona, Tucson, AZ 85721, USA
* Author to whom correspondence should be addressed.
Received: 8 October 2013; in revised form: 1 December 2013 / Accepted: 3 December 2013 / Published: 13 December 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Cytochrome P450 monooxygenases (CYPs or P450s) play paramount roles in detoxification of insecticides in a number of insect pests. However, little is known about the roles of P450s and their responses to insecticide exposure in the codling moth Cydia pomonella (L.), an economically important fruit pest. Here we report the characterization and expression analysis of the first P450 gene, designated as CYP9A61, from this pest. The full-length cDNA sequence of CYP9A61 is 2071 bp long and its open reading frame (ORF) encodes 538 amino acids. Sequence analysis shows that CYP9A61 shares 51%–60% identity with other known CYP9s and contains the highly conserved substrate recognition site SRS1, SRS4 and SRS5. Quantitative real-time PCR showed that CYP9A61 were 67-fold higher in the fifth instar larvae than in the first instar, and more abundant in the silk gland and fat body than other tissues. Exposure of the 3rd instar larvae to 12.5 mg L−1 of chlorpyrifos-ethyl for 60 h and 0.19 mg L−1 of lambda-cyhalothrin for 36 h resulted in 2.20- and 3.47-fold induction of CYP9A61, respectively. Exposure of the 3rd instar larvae to these two insecticides also significantly enhanced the total P450 activity. The results suggested that CYP9A61 is an insecticide-detoxifying P450.
Keywords: Cydia pomonella; cytochrome P450; induction; response; insecticides

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MDPI and ACS Style

Yang, X.; Li, X.; Zhang, Y. Molecular Cloning and Expression of CYP9A61: A Chlorpyrifos-Ethyl and Lambda-Cyhalothrin-Inducible Cytochrome P450 cDNA from Cydia pomonella. Int. J. Mol. Sci. 2013, 14, 24211-24229.

AMA Style

Yang X, Li X, Zhang Y. Molecular Cloning and Expression of CYP9A61: A Chlorpyrifos-Ethyl and Lambda-Cyhalothrin-Inducible Cytochrome P450 cDNA from Cydia pomonella. International Journal of Molecular Sciences. 2013; 14(12):24211-24229.

Chicago/Turabian Style

Yang, Xueqing; Li, Xianchun; Zhang, Yalin. 2013. "Molecular Cloning and Expression of CYP9A61: A Chlorpyrifos-Ethyl and Lambda-Cyhalothrin-Inducible Cytochrome P450 cDNA from Cydia pomonella." Int. J. Mol. Sci. 14, no. 12: 24211-24229.

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