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Differential Expression Analysis of a Subset of Drought-Responsive GmNAC Genes in Two Soybean Cultivars Differing in Drought Tolerance
School of Biotechnology, International University, Vietnam National University HCMC, Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh 70000, Vietnam
Signaling Pathway Research Unit, RIKEN Center for Sustainable Resource Science, 1-7-22, Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan
National Key Laboratory of Plant Cell Biotechnology, Agricultural Genetics Institute, Vietnamese Academy of Agricultural Science, Pham Van Dong Street, Hanoi 10000, Vietnam
Post-Graduate Program, Vietnamese Academy of Agricultural Science, Thanhtri, Hanoi 10000, Vietnam
These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 23 October 2013; in revised form: 20 November 2013 / Accepted: 20 November 2013 / Published: 6 December 2013
Abstract: The plant-specific NAC transcription factors play important roles in plant response to drought stress. Here, we have compared the expression levels of a subset of GmNAC genes in drought-tolerant DT51 and drought-sensitive MTD720 under both normal and drought stress conditions aimed at identifying correlation between GmNAC expression levels and drought tolerance degree, as well as potential GmNAC candidates for genetic engineering. The expression of 23 selected dehydration-responsive GmNACs was assessed in both stressed and unstressed root tissues of DT51 and MTD720 using real-time quantitative PCR. The results indicated that expression of GmNACs was genotype-dependent. Seven and 13 of 23 tested GmNACs showed higher expression levels in roots of DT51 in comparison with MTD720 under normal and drought stress conditions, respectively, whereas none of them displayed lower transcript levels under any conditions. This finding suggests that the higher drought tolerance of DT51 might be positively correlated with the higher induction of the GmNAC genes during water deficit. The drought-inducible GmNAC011 needs to be mentioned as its transcript accumulation was more than 76-fold higher in drought-stressed DT51 roots relative to MTD720 roots. Additionally, among the GmNAC genes examined, GmNAC085, 092, 095, 101 and 109 were not only drought-inducible but also more highly up-regulated in DT51 roots than in that of MTD720 under both treatment conditions. These data together suggest that GmNAC011, 085, 092, 095, 101 and 109 might be promising candidates for improvement of drought tolerance in soybean by biotechnological approaches.
Keywords: comparative expression analysis; drought stress; NAC transcription factor; real-time quantitative PCR; soybean
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Thao, N.P.; Thu, N.B.A.; Hoang, X.L.T.; Ha, C.V.; Tran, L.-S.P. Differential Expression Analysis of a Subset of Drought-Responsive GmNAC Genes in Two Soybean Cultivars Differing in Drought Tolerance. Int. J. Mol. Sci. 2013, 14, 23828-23841.
Thao NP, Thu NBA, Hoang XLT, Ha CV, Tran L-SP. Differential Expression Analysis of a Subset of Drought-Responsive GmNAC Genes in Two Soybean Cultivars Differing in Drought Tolerance. International Journal of Molecular Sciences. 2013; 14(12):23828-23841.
Thao, Nguyen P.; Thu, Nguyen B.A.; Hoang, Xuan L.T.; Ha, Chien V.; Tran, Lam-Son P. 2013. "Differential Expression Analysis of a Subset of Drought-Responsive GmNAC Genes in Two Soybean Cultivars Differing in Drought Tolerance." Int. J. Mol. Sci. 14, no. 12: 23828-23841.