Next Article in Journal
Systems Biology Approach to the Dissection of the Complexity of Regulatory Networks in the S. scrofa Cardiocirculatory System
Previous Article in Journal
Detection of Lymph Node Metastases in Human Colorectal Cancer by Using 5-Aminolevulinic Acid-Induced Protoporphyrin IX Fluorescence with Spectral Unmixing
Int. J. Mol. Sci. 2013, 14(11), 23153-23159; doi:10.3390/ijms141123153
Article

Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification

1,* , 2
,
3
,
3
 and
3
1 National Research Institute of Aquaculture, Fisheries Research Agency, Nikko, Tochigi 321-1661, Japan 2 Tohoku National Fisheries Research Institute, Fisheries Research Agency, Shiogama, Miyagi 985-0001, Japan 3 National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, Kanagawa 236-8648, Japan
* Author to whom correspondence should be addressed.
Received: 26 September 2013 / Revised: 11 November 2013 / Accepted: 12 November 2013 / Published: 21 November 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [206 KB, uploaded 19 June 2014]

Abstract

We developed tetranucleotide-repeat microsatellite markers for the masu salmon (Oncorhynchus masou) complex. 454 pyrosequencing was used to discover repeat motifs, and seven polymorphic microsatellite-primer sets were identified. The number of alleles detected at each locus ranged from four to 24 and the expected heterozygosity varied from 0.57 to 0.92. Cross-subspecies amplification for O. m. masou, O. m. ishikawae and O. m. subsp. was successful. These microsatellites can be utilized in studies of genetic structure, genetic diversity, and intra- and inter-subspecific hybridization, making a contribution to conservation and management of the Oncorhynchus masou complex.
Keywords: short tandem repeat; simple sequence repeat; 454 pyrosequencing; next-generation sequencer; Oncorhynchus masou short tandem repeat; simple sequence repeat; 454 pyrosequencing; next-generation sequencer; Oncorhynchus masou
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Yamamoto, S.; Kurokawa, T.; Sekino, M.; Yasuike, M.; Saitoh, K. Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification. Int. J. Mol. Sci. 2013, 14, 23153-23159.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert