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Int. J. Mol. Sci. 2013, 14(11), 23153-23159; doi:10.3390/ijms141123153

Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification

1
National Research Institute of Aquaculture, Fisheries Research Agency, Nikko, Tochigi 321-1661, Japan
2
Tohoku National Fisheries Research Institute, Fisheries Research Agency, Shiogama, Miyagi 985-0001, Japan
3
National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, Kanagawa 236-8648, Japan
*
Author to whom correspondence should be addressed.
Received: 26 September 2013 / Revised: 11 November 2013 / Accepted: 12 November 2013 / Published: 21 November 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

We developed tetranucleotide-repeat microsatellite markers for the masu salmon (Oncorhynchus masou) complex. 454 pyrosequencing was used to discover repeat motifs, and seven polymorphic microsatellite-primer sets were identified. The number of alleles detected at each locus ranged from four to 24 and the expected heterozygosity varied from 0.57 to 0.92. Cross-subspecies amplification for O. m. masou, O. m. ishikawae and O. m. subsp. was successful. These microsatellites can be utilized in studies of genetic structure, genetic diversity, and intra- and inter-subspecific hybridization, making a contribution to conservation and management of the Oncorhynchus masou complex. View Full-Text
Keywords: short tandem repeat; simple sequence repeat; 454 pyrosequencing; next-generation sequencer; Oncorhynchus masou short tandem repeat; simple sequence repeat; 454 pyrosequencing; next-generation sequencer; Oncorhynchus masou
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Yamamoto, S.; Kurokawa, T.; Sekino, M.; Yasuike, M.; Saitoh, K. Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification. Int. J. Mol. Sci. 2013, 14, 23153-23159.

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