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Int. J. Mol. Sci. 2013, 14(11), 23153-23159; doi:10.3390/ijms141123153
Article

Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification

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Received: 26 September 2013; in revised form: 11 November 2013 / Accepted: 12 November 2013 / Published: 21 November 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: We developed tetranucleotide-repeat microsatellite markers for the masu salmon (Oncorhynchus masou) complex. 454 pyrosequencing was used to discover repeat motifs, and seven polymorphic microsatellite-primer sets were identified. The number of alleles detected at each locus ranged from four to 24 and the expected heterozygosity varied from 0.57 to 0.92. Cross-subspecies amplification for O. m. masou, O. m. ishikawae and O. m. subsp. was successful. These microsatellites can be utilized in studies of genetic structure, genetic diversity, and intra- and inter-subspecific hybridization, making a contribution to conservation and management of the Oncorhynchus masou complex.
Keywords: short tandem repeat; simple sequence repeat; 454 pyrosequencing; next-generation sequencer; Oncorhynchus masou short tandem repeat; simple sequence repeat; 454 pyrosequencing; next-generation sequencer; Oncorhynchus masou
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yamamoto, S.; Kurokawa, T.; Sekino, M.; Yasuike, M.; Saitoh, K. Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification. Int. J. Mol. Sci. 2013, 14, 23153-23159.

AMA Style

Yamamoto S, Kurokawa T, Sekino M, Yasuike M, Saitoh K. Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification. International Journal of Molecular Sciences. 2013; 14(11):23153-23159.

Chicago/Turabian Style

Yamamoto, Shoichiro; Kurokawa, Tadahide; Sekino, Masashi; Yasuike, Motoshige; Saitoh, Kenji. 2013. "Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification." Int. J. Mol. Sci. 14, no. 11: 23153-23159.



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