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Int. J. Mol. Sci. 2013, 14(11), 23033-23044; doi:10.3390/ijms141123033
Article

Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and l-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles

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Received: 12 October 2013; in revised form: 12 November 2013 / Accepted: 13 November 2013 / Published: 20 November 2013
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Abstract: It has been demonstrated that the content of certain amino acids in eggs is not sufficient to fully support embryonic development. One possibility to supply the embryo with extra nutrients and energy is in ovo administration of nutrients. Nanoparticles of diamond are highly biocompatible non-toxic carbonic structures, and we hypothesized that bio-complexes of diamond nanoparticles with l-glutamine may affect molecular responses in breast muscle. The objective of the investigation was to evaluate the effect of diamond nanoparticle (ND) and l-glutamine (Gln) on expression of growth and differentiation factors of chicken embryo pectoral muscles. ND, Gln, and Gln/ND solutions (50 mg/L) were injected into fertilized broiler chicken eggs at the beginning of embryogenesis. Muscle tissue was dissected at day 20 of incubation and analysed for gene expression of FGF2, VEGF-A, and MyoD1. ND and especially Gln/ND up-regulated expression of genes related to muscle cell proliferation (FGF2) and differentiation (MyoD1). Furthermore, the ratio between FGF2 and MyoD1 was highest in the Gln/ND group. At the end of embryogenesis, Gln/ND enhanced both proliferation and differentiation of pectoral muscle cells and differentiation dominated over proliferation. These preliminary results suggest that the bio-complex of glutamine and diamond nanoparticles may accelerate growth and maturation of muscle cells.
Keywords: chicken embryo; myogenesis; diamond nanoparticles; l-glutamine; gene expression chicken embryo; myogenesis; diamond nanoparticles; l-glutamine; gene expression
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Grodzik, M.; Sawosz, F.; Sawosz, E.; Hotowy, A.; Wierzbicki, M.; Kutwin, M.; Jaworski, S.; Chwalibog, A. Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and l-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles. Int. J. Mol. Sci. 2013, 14, 23033-23044.

AMA Style

Grodzik M, Sawosz F, Sawosz E, Hotowy A, Wierzbicki M, Kutwin M, Jaworski S, Chwalibog A. Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and l-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles. International Journal of Molecular Sciences. 2013; 14(11):23033-23044.

Chicago/Turabian Style

Grodzik, Marta; Sawosz, Filip; Sawosz, Ewa; Hotowy, Anna; Wierzbicki, Mateusz; Kutwin, Marta; Jaworski, Sławomir; Chwalibog, André. 2013. "Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and l-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles." Int. J. Mol. Sci. 14, no. 11: 23033-23044.



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