Int. J. Mol. Sci. 2013, 14(11), 22721-22740; doi:10.3390/ijms141122721

Genes Responsive to Low-Intensity Pulsed Ultrasound in MC3T3-E1 Preosteoblast Cells

1,* email, 1email, 2email, 3email, 4email and 5email
Received: 16 April 2013; in revised form: 4 July 2013 / Accepted: 6 August 2013 / Published: 18 November 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Although low-intensity pulsed ultrasound (LIPUS) has been shown to enhance bone fracture healing, the underlying mechanism of LIPUS remains to be fully elucidated. Here, to better understand the molecular mechanism underlying cellular responses to LIPUS, we investigated gene expression profiles in mouse MC3T3-E1 preosteoblast cells exposed to LIPUS using high-density oligonucleotide microarrays and computational gene expression analysis tools. Although treatment of the cells with a single 20-min LIPUS (1.5 MHz, 30 mW/cm2) did not affect the cell growth or alkaline phosphatase activity, the treatment significantly increased the mRNA level of Bglap. Microarray analysis demonstrated that 38 genes were upregulated and 37 genes were downregulated by 1.5-fold or more in the cells at 24-h post-treatment. Ingenuity pathway analysis demonstrated that the gene network U (up) contained many upregulated genes that were mainly associated with bone morphology in the category of biological functions of skeletal and muscular system development and function. Moreover, the biological function of the gene network D (down), which contained downregulated genes, was associated with gene expression, the cell cycle and connective tissue development and function. These results should help to further clarify the molecular basis of the mechanisms of the LIPUS response in osteoblast cells.
Keywords: low-intensity pulsed ultrasound; osteoblast; microarray; gene expression; gene network
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MDPI and ACS Style

Tabuchi, Y.; Sugahara, Y.; Ikegame, M.; Suzuki, N.; Kitamura, K.-I.; Kondo, T. Genes Responsive to Low-Intensity Pulsed Ultrasound in MC3T3-E1 Preosteoblast Cells. Int. J. Mol. Sci. 2013, 14, 22721-22740.

AMA Style

Tabuchi Y, Sugahara Y, Ikegame M, Suzuki N, Kitamura K-I, Kondo T. Genes Responsive to Low-Intensity Pulsed Ultrasound in MC3T3-E1 Preosteoblast Cells. International Journal of Molecular Sciences. 2013; 14(11):22721-22740.

Chicago/Turabian Style

Tabuchi, Yoshiaki; Sugahara, Yuuki; Ikegame, Mika; Suzuki, Nobuo; Kitamura, Kei-ichiro; Kondo, Takashi. 2013. "Genes Responsive to Low-Intensity Pulsed Ultrasound in MC3T3-E1 Preosteoblast Cells." Int. J. Mol. Sci. 14, no. 11: 22721-22740.

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