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Int. J. Mol. Sci. 2013, 14(11), 22233-22245; doi:10.3390/ijms141122233
Article

Galectin-1 Is an Interactive Protein of Selenoprotein M in the Brain

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Received: 4 September 2013; in revised form: 24 October 2013 / Accepted: 24 October 2013 / Published: 11 November 2013
(This article belongs to the Special Issue Nutritional Control of Metabolism)
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Abstract: Selenium, an essential trace element for human health, mainly exerts its biological function through selenoproteins. Selenoprotein M (SelM) is one of the highly expressed selenoproteins in the brain, but its biological effect and molecular mechanism remain unclear. Thus, the interactive protein of SelM was investigated in this paper to guide further study. In order to avoid protein translational stop, the selenocysteine-encoding UGA inside the open reading frame of SelM was site-directly changed to the cysteine-encoding UGC to generate the SelM' mutant. Meanwhile, its N terminal transmembrane signal peptide was also cut off. This truncated SelM' was used to screen a human fetal brain cDNA library by the yeast two-hybrid system. A new interactive protein of SelM' was found to be galectin-1 (Gal-1). This protein-protein interaction was further verified by the results of fluorescence resonance energy transfer techniques, glutathione S-transferase pull-down and co-immunoprecipitation assays. As Gal-1 plays important roles in preventing neurodegeneration and promoting neuroprotection in the brain, the interaction between SelM' and Gal-1 displays a new direction for studying the biological function of SelM in the human brain.
Keywords: selenoprotein M (SelM); galectin-1 (Gal-1); protein-protein interaction; yeast two-hybridization; fluorescence resonance energy transfer (FRET); co-immunoprecipitation; Glutathione S-transferase pull-down (GST pull-down) selenoprotein M (SelM); galectin-1 (Gal-1); protein-protein interaction; yeast two-hybridization; fluorescence resonance energy transfer (FRET); co-immunoprecipitation; Glutathione S-transferase pull-down (GST pull-down)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Qiao, X.; Tian, J.; Chen, P.; Wang, C.; Ni, J.; Liu, Q. Galectin-1 Is an Interactive Protein of Selenoprotein M in the Brain. Int. J. Mol. Sci. 2013, 14, 22233-22245.

AMA Style

Qiao X, Tian J, Chen P, Wang C, Ni J, Liu Q. Galectin-1 Is an Interactive Protein of Selenoprotein M in the Brain. International Journal of Molecular Sciences. 2013; 14(11):22233-22245.

Chicago/Turabian Style

Qiao, Xifeng; Tian, Jing; Chen, Ping; Wang, Chao; Ni, Jiazuan; Liu, Qiong. 2013. "Galectin-1 Is an Interactive Protein of Selenoprotein M in the Brain." Int. J. Mol. Sci. 14, no. 11: 22233-22245.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert