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Galectin-1 Is an Interactive Protein of Selenoprotein M in the Brain
College of Life Sciences, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen 518060, China
Department of Marine Biology, Shenzhen Key Laboratory of Marine Biotechnology and Ecology, Shenzhen University, Shenzhen 518060, China
Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
University of Chinese Academy of Sciences, Beijing 100049, China
* Author to whom correspondence should be addressed.
Received: 4 September 2013; in revised form: 24 October 2013 / Accepted: 24 October 2013 / Published: 11 November 2013
Abstract: Selenium, an essential trace element for human health, mainly exerts its biological function through selenoproteins. Selenoprotein M (SelM) is one of the highly expressed selenoproteins in the brain, but its biological effect and molecular mechanism remain unclear. Thus, the interactive protein of SelM was investigated in this paper to guide further study. In order to avoid protein translational stop, the selenocysteine-encoding UGA inside the open reading frame of SelM was site-directly changed to the cysteine-encoding UGC to generate the SelM' mutant. Meanwhile, its N terminal transmembrane signal peptide was also cut off. This truncated SelM' was used to screen a human fetal brain cDNA library by the yeast two-hybrid system. A new interactive protein of SelM' was found to be galectin-1 (Gal-1). This protein-protein interaction was further verified by the results of fluorescence resonance energy transfer techniques, glutathione S-transferase pull-down and co-immunoprecipitation assays. As Gal-1 plays important roles in preventing neurodegeneration and promoting neuroprotection in the brain, the interaction between SelM' and Gal-1 displays a new direction for studying the biological function of SelM in the human brain.
Keywords: selenoprotein M (SelM); galectin-1 (Gal-1); protein-protein interaction; yeast two-hybridization; fluorescence resonance energy transfer (FRET); co-immunoprecipitation; Glutathione S-transferase pull-down (GST pull-down)
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MDPI and ACS Style
Qiao, X.; Tian, J.; Chen, P.; Wang, C.; Ni, J.; Liu, Q. Galectin-1 Is an Interactive Protein of Selenoprotein M in the Brain. Int. J. Mol. Sci. 2013, 14, 22233-22245.
Qiao X, Tian J, Chen P, Wang C, Ni J, Liu Q. Galectin-1 Is an Interactive Protein of Selenoprotein M in the Brain. International Journal of Molecular Sciences. 2013; 14(11):22233-22245.
Qiao, Xifeng; Tian, Jing; Chen, Ping; Wang, Chao; Ni, Jiazuan; Liu, Qiong. 2013. "Galectin-1 Is an Interactive Protein of Selenoprotein M in the Brain." Int. J. Mol. Sci. 14, no. 11: 22233-22245.