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Int. J. Mol. Sci. 2013, 14(11), 22246-22257; doi:10.3390/ijms141122246

Enhancing Metagenomics Investigations of Microbial Interactions with Biofilm Technology

Department of Biology, Texas State University, 601 University Drive, San Marcos, TX 78666, USA
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Received: 15 September 2013 / Revised: 25 October 2013 / Accepted: 29 October 2013 / Published: 11 November 2013
(This article belongs to the Special Issue Biofilms: Extracellular Bastions of Bacteria)
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Abstract

Investigations of microbial ecology and diversity have been greatly enhanced by the application of culture-independent techniques. One such approach, metagenomics, involves sample collections from soil, water, and other environments. Extracted nucleic acids from bulk environmental samples are sequenced and analyzed, which allows microbial interactions to be inferred on the basis of bioinformatics calculations. In most environments, microbial interactions occur predominately in surface-adherent, biofilm communities. In this review, we address metagenomics sampling and biofilm biology, and propose an experimental strategy whereby the resolving power of metagenomics can be enhanced by incorporating a biofilm-enrichment step during sample acquisition.
Keywords: biofilm-enrichment; metagenomics; functional screening; sequence-based screening; microbial interactions biofilm-enrichment; metagenomics; functional screening; sequence-based screening; microbial interactions
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

McLean, R.J.C.; Kakirde, K.S. Enhancing Metagenomics Investigations of Microbial Interactions with Biofilm Technology. Int. J. Mol. Sci. 2013, 14, 22246-22257.

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